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Method for detecting Advantame in food by using deuterium band internal standard substance

An internal standard and food technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unseen detection of advantame

Pending Publication Date: 2022-06-03
大连市检验检测认证技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In domestic and foreign testing standards and literature, there is no method to detect advantame in food by using deuterium band internal standard

Method used

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  • Method for detecting Advantame in food by using deuterium band internal standard substance
  • Method for detecting Advantame in food by using deuterium band internal standard substance
  • Method for detecting Advantame in food by using deuterium band internal standard substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The mensuration of Advanta sweet in embodiment 1 frozen drink - deuterium band internal standard method

[0062] (1) Sample pretreatment

[0063] Accurately weigh 2g of the sample (accurate to 0.01g), put it in a 50mL centrifuge tube with stopper, add 200μL of Advantame-D3 standard intermediate solution with a concentration of 10μg / mL, add 3mL of water, vortex for 30s, add 2g After NaCl, add 20 mL of acetonitrile, centrifuge at 12000 r / min for 3 min, take 2 mL of acetonitrile layer into a centrifuge tube, add 50 mg of C 18 , vortexed for 1min, centrifuged at 12000r / min for 5min, took the supernatant, passed through a 0.22 μm filter membrane, and analyzed by liquid chromatography-tandem mass spectrometer.

[0064] (2) Preparation of standard solution and blank solution

[0065] ① Advansame standard stock solution: Weigh 10 mg of Advansame standard substance (accurate to 0.01 mg), dissolve in a 10 mL volumetric flask, and dilute to the mark with methanol to obtain a sta...

Embodiment 2

[0104] The mensuration of Advanta sweet in embodiment 2 baked goods - deuterium band internal standard method

[0105] (1) Take the bakery food sample to be tested, the pretreatment method, the preparation method of the standard solution and the measurement method are the same as those in Example 1. The obtained deuterium band internal standard method calibration curve is shown in Table 6, Image 6 shown.

[0106] (2) Standard recovery and repeatability

[0107] Add 10 μg / kg, 20 μg / kg, 100 μg / kg three levels of Advansame standard solution to the bakery food without Advansame respectively, and then carry out content determination according to the above steps after standing at room temperature for 30 min. Each level of addition was measured in 6 replicates. The measured results were compared with the theoretical added concentration to obtain the average recovery rate and relative standard deviation. The results are shown in Table 8.

[0108] Table 8 Recovery and precision of...

Embodiment 3

[0113] Determination of Adewan Sweet in embodiment 3 seasonings - deuterium band internal standard method

[0114] (1) Take the seasoning sample to be tested, the pretreatment method, the preparation method of the standard solution and the measurement method are the same as those in Example 1. The obtained deuterium band internal standard method calibration curve is shown in Table 6, Image 6 shown.

[0115] (2) Standard recovery and repeatability

[0116] Add 10 μg / kg, 20 μg / kg, 100 μg / kg three levels of Advansame standard solution to the seasoning without Advansame respectively, and then carry out content determination according to the above steps after standing at room temperature for 30 min. Each level of addition was measured in 6 replicates. The measured results were compared with the theoretical added concentration to obtain the average recovery rate and relative standard deviation. The results are shown in Table 9.

[0117] Table 9 Recovery and precision of Advanta...

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Abstract

The invention relates to a method for detecting Advantame in food by using a deuterium band internal standard substance, belonging to the field of food detection. The method comprises the following steps: firstly, preparing standard solutions including (1) an Advantame standard stock solution, (2) an Advantame standard intermediate solution, (3) an Advantame-D3 standard stock solution, (4) an Advantame-D3 standard intermediate solution and (5) a standard working solution: respectively transferring the Advantame standard intermediate solutions; secondly, pre-treating the sample, and determining reference conditions of liquid chromatography-tandem mass spectrometry analysis; thirdly, sequentially performing qualitative and quantitative determination on the basis of liquid chromatography-tandem mass spectrometry analysis reference conditions and the prepared standard solution. The method has the advantages of accuracy, high efficiency and rapidness.

Description

technical field [0001] The invention belongs to the field of food detection, and relates to a detection method, in particular to a method for detecting Advanta in food by using a deuterium band internal standard substance. Background technique [0002] Advantame (Advantame, N-[N-[3-(3-hydroxy-4-methoxyphenyl)propyl-L-a-aspartyl]-L-phenylalanine-1-methyl ester ]) is an ultra-high intensity sweetener, a derivative of aspartame. Advansame has a sensory taste similar to aspartame, but its sweetness is 100 times that of aspartame and 20,000 times that of sucrose, and its bitterness and sourness are very mild. Therefore, Advansame can replace sugars (sucrose, glucose, fructose, etc.) to replace the existing high-intensity sweeteners used in the food industry, and is a multi-purpose, low-energy new type of sweetener. [0003] At present, the related detection of Advanta at home and abroad mainly include liquid chromatography and liquid chromatography tandem mass spectrometry, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8679G01N2030/045
Inventor 勇艳华边海涛毛希琴李莉
Owner 大连市检验检测认证技术服务中心
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