Application of radix arnebiae seu lithospermi extract in preparation of medicine for regulating reproductive tract flora disorder
A comfrey extract, reproductive tract technology, applied in the direction of drug combination, plant/algae/fungus/moss components, pharmaceutical formulations, etc., to achieve a good effect of preventing and/or treating diseases related to reproductive tract flora disorder
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Embodiment 1
[0020] Example 1 Preparation of Comfrey Extract
[0021] After crushing the dried comfrey roots whose quality meets the relevant regulations in the 2020 edition of the Chinese Pharmacopoeia, take 4 g and 400 mL of distilled water and place them in a 500 mL round-bottom flask, reflux and extract 3 times at 100 °C for 3 h each time, filter, and combine 3 The secondary filtrate was freeze-dried to obtain the comfrey extract. Before use, the corresponding concentration of the drug solution was prepared with PBS.
Embodiment 2
[0022] Example 2 Comfrey extract regulates the microflora of the genital tract of mice
[0023] This experiment passed the ethical review of the Laboratory Animal Ethics Committee of the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences. C57BL / 6J mice were purchased from Guangdong Yaokang Biotechnology Co., Ltd.; comycin, imipenem / cilastatin and neomycin were purchased from Shanghai Yuanye Biotechnology Co., Ltd.
[0024] Thirty healthy C57BL / 6J female mice were adaptively fed for 1 week, and the mice were randomly divided into 3 groups with 10 mice in each group. The blank control group was given 100 μL PBS every day; the antibiotic group and the comfrey extract group were given 100 μL antibiotics (500 mg / L vancomycin, 500 mg / L imipenem / cilastatin, 1 g / L neomycin) every day. ); after gavage for three weeks, each mouse in the blank control group and antibiotic group was injected with 100 μL of PBS into the vagina every day; in the comfrey extract grou...
Embodiment 3
[0026] Example 3 The effect of comfrey extract on the aggregation of neutrophils and macrophages to the inflammation of zebrafish caudal fin
[0027] The healthy zebrafish Tg(corola:eGFP) that developed to 3dpf (days post fertilization) were selected and placed in a 6-well cell culture plate, 25 cells / well, the blank control group and the 1 group of comfrey extract were added with PBS, and pretreated with antibiotics Antibiotics (10 μg / mL vancomycin, 10 μg / mL imipenem / cilastatin, 10 μg / mL neomycin) were added to the group, antibiotic group, 2 groups of comfrey extract, and 3 groups of comfrey extract. 3 mL per well, fresh solution was replaced every day, after 3 days of treatment, the caudal fins of zebrafish were cut off with a scalpel under a stereo microscope, and then placed in a 6-well cell culture plate, 20 cells / well, then blank control group and antibiotic pre-treatment The treatment group was added PBS, the antibiotic group was added antibiotics (10 μg / mL vancomycin, ...
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