Preparation method of wood frog bone and meat polypeptide chelated zinc microcapsule

A technology for chelating zinc and microcapsules is applied in the field of preparation of Rana grove bone and flesh polypeptide chelated zinc microcapsules, which can solve problems such as low utilization rate, and achieve the effects of improving content, protecting biological activity and promoting zinc absorption.

Pending Publication Date: 2022-06-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To provide a solution to the low utilization rate of wood frog

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Pretreatment: Take 30 g of Rana Rana bone and flesh residues, wash them, and then dry them in an oven at 60°C for 1 hour. The dried Rana wood residues were pulverized for 5 minutes and passed through a 50-mesh sieve to obtain 26.3 g of Rana wood flesh powder.

[0029] (2) Degreasing: Put the Rana grove bone and meat powder in a beaker, add 263mL of 0.5mol / L N a HCO 3 The solution was soaked overnight. The pellet is then retained after centrifugation and washing.

[0030] (3) Enzymatic hydrolysis: 263 mL of distilled water was added to the defatted Rana sylvatica bone and meat powder, and 1.315 g of trypsin was used for enzymatic hydrolysis for 2 hours. The enzymatic hydrolysis temperature was 40° C. and pH=8.0. Then, 2.63 g of papain was added for enzymatic hydrolysis again for 90 min, and the enzymatic hydrolysis temperature was 60° C. and pH=8.0. After sufficient enzymatic hydrolysis, the enzymatic hydrolysis solution was heated to 95°C for 15 min to inactivat...

Embodiment 2

[0036] (1) Pretreatment: Take 50 g of Rana sylvatica bone and flesh residue, wash it, and then dry it in an oven at 60°C for 1 hour. The dried Rana wood residues were pulverized for 5 minutes and passed through a 50-mesh sieve to obtain 43.1 g of Rana wood flesh powder.

[0037] (2) Degreasing: Put the Rana grove bone and meat powder in a beaker, add 431mL of 0.5mol / L N a HCO 3 The solution was soaked overnight. The pellet is then retained after centrifugation and washing.

[0038] (3) Enzymatic hydrolysis: 431 mL of distilled water was added to the defatted Rana sylvatica bone and meat powder, and 2.155 g of trypsin was used for enzymatic hydrolysis for 2 hours. The enzymatic hydrolysis temperature was 40° C. and pH=8.0. Then, 4.31 g of papain was added for enzymatic hydrolysis again for 90 min, and the enzymatic hydrolysis temperature was 60° C. and pH=8.0. After sufficient enzymatic hydrolysis, the enzymatic hydrolysis solution was heated to 95°C for 15 min to inactivat...

Embodiment 3

[0044](1) Pretreatment: Take 100 g of Rana sylvatica bone and flesh residues, wash them, and then dry them in an oven at 60°C for 1 hour. The dried Rana wood residues were pulverized for 5 minutes and passed through a 50-mesh sieve to obtain 88.8 g of Rana wood flesh powder.

[0045] (2) Degreasing: Put the Rana grove bone and meat powder in a beaker, add 888mL of 0.5mol / L N a HCO 3 The solution was soaked overnight. The pellet is then retained after centrifugation and washing.

[0046] (3) Enzymatic hydrolysis: 888 mL of distilled water was added to the defatted Rana sylvatica bone and meat powder, and 4.44 g of trypsin was used for enzymatic hydrolysis for 2 hours. The enzymatic hydrolysis temperature was 40° C. and pH=8.0. After that, 8.88 g of papain was added for enzymatic hydrolysis again for 90 min, and the enzymatic hydrolysis temperature was 60° C. and pH=8.0. After sufficient enzymatic hydrolysis, the enzymatic hydrolysis solution was heated to 95°C for 15 min to...

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Abstract

The invention belongs to the field of application of wood frogs to deep processing of wood frog bone and meat, and discloses a preparation method of a wood frog bone and meat polypeptide chelated zinc microcapsule and a microcapsule prepared from the wood frog bone and meat polypeptide chelated zinc microcapsule through an embedding technology. The method comprises the following steps: 1, pretreating wood frog residues; 2, degreasing; 3, performing enzymolysis to obtain wood frog polypeptide liquid; 4, performing ultrafiltration to obtain small molecule polypeptide; 5, preparing polypeptide chelated zinc; 6, precipitation of polypeptide chelated zinc through alcohol precipitation; and 7, preparing the microcapsule. As a novel trace element supplement, the polypeptide chelated zinc not only can avoid zinc precipitation, but also can be absorbed through an intestinal peptide absorption way and interacts with the intestinal peptide absorption way to regulate proliferation and differentiation of intestinal cells, so that the utilization rate of an organism is increased. The wood frog bone and meat polypeptide chelated zinc microcapsule prepared by the invention is expected to provide a feasible zinc supplementing scheme for zinc deficiency patients and improve the comprehensive utilization rate of wood frogs.

Description

technical field [0001] The invention belongs to the field of deep processing of Rana wood products, and studies a preparation method of Rana Rana bone and flesh polypeptide chelated zinc microcapsules. Background technique [0002] The precious frog species of Rana in northeast my country has been recorded in the "Compendium of Materia Medica" for its pharmacological effects, and its Rana Rana oil is known as one of the three treasures of Northeast China. However, the current deep processing of Rana wood products is mainly based on Rana Rana oil, while the flesh and bones of Rana Rana are mostly discarded. This not only pollutes the environment, but also wastes resources. Therefore, how to improve the deep processing and added value of Rana wood products, so as to create greater economic and ecological benefits, has always been one of the important issues in the field of Rana wood products processing. [0003] Zinc is a trace element necessary for the human body, and its c...

Claims

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Application Information

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IPC IPC(8): A23L33/165B01J13/02
CPCA23L33/165B01J13/02A23V2002/00A23V2200/30A23V2250/55A23V2250/1642
Inventor 关爽葛广财卢静
Owner JILIN UNIV
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