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Functional low-lactose prebiotic liquid goat milk and preparation method thereof

A low-lactose, prebiotic technology, applied in dairy products, milk preparations, applications, etc., can solve the problems of low lactose content of transglycosidic active lactase, synthesis of galactose into galactooligosaccharides, high content of galactooligosaccharides, etc. , to increase the content of galacto-oligosaccharides, improve the hydrolysis rate of lactose, and improve the taste

Pending Publication Date: 2022-06-17
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The full name of lactase is β-D-galactoside galactohydrolase, which exists in animals, plants and microorganisms, but the properties of lactases of different microorganisms are quite different, their optimum pH and the hydrolysis efficiency of lactose It is also different. Some lactases only have the ability to hydrolyze lactose, and their transglycoside activity is low, so they cannot synthesize galactose into galactooligosaccharides. Some lactases have strong transglycoside activity, and can synthesize galactose into galactooligosaccharides (Galacto- oligosaccharide, GOS), is a prebiotic that can promote the proliferation of intestinal flora and facilitate mineral absorption
At present, low-lactose dairy products on the market are mainly hydrolyzed by a single enzyme, the purpose of which is to reduce the concentration of lactose, relieve and prevent lactose intolerance, and do not consider the compounding of hydrolytic activity and transglycosidic activity of lactase to prepare low-lactose content and oligomeric half Functional prebiotic dairy products high in lactose

Method used

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  • Functional low-lactose prebiotic liquid goat milk and preparation method thereof
  • Functional low-lactose prebiotic liquid goat milk and preparation method thereof
  • Functional low-lactose prebiotic liquid goat milk and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Add lactase K and lactase P in a volume ratio of 3:1 to the goat milk that is pasteurized at 65°C for 15 minutes and cooled to 50°C, so that the final lactase content is 0.8 g / L;

[0028] Step 2: Add γ-aminobutyric acid and N-acetylneuraminic acid to make the final concentrations 2g / L and 1g / L respectively, stir evenly and then enzymolysis at a constant temperature of 50°C for 3h;

[0029] Step 3. Add Baofeng seed oil and phytosterols to make the concentrations 3g / L and 5g / L respectively, homogenize under high pressure for 10min, keep at 140°C for 2s after sterilization, and pack to obtain functional low-lactose prebiotic liquid goat milk. The prepared functional prebiotic liquid goat milk has a shelf life of 60 days at room temperature.

[0030] The lactose / galactose kit was used to measure the content of lactose and galactose in the goat milk before and after the enzymatic hydrolysis of Example 1, and it was detected that the lactose content in the functional ...

Embodiment 2

[0032] Step 1. Lactase K and lactase P are added to the goat milk that is pasteurized at 65°C for 15 minutes and cooled to 50°C in a volume ratio of 2:1, so that the final lactase content is 0.6 g / L;

[0033] Step 2: Add γ-aminobutyric acid and N-acetylneuraminic acid to make the final concentrations 2g / L and 1g / L respectively, stir evenly and then enzymolysis at 45°C for 2h;

[0034] Step 3, adding Baofeng seed oil and phytosterols to make the concentrations 3g / L and 5g / L respectively, homogenizing under high pressure for 10min, sterilizing at 85°C for 10s, and packaging to obtain functional low-lactose prebiotic liquid goat milk, The prepared functional prebiotic liquid goat milk was refrigerated at 4°C for 7 days.

[0035] The lactose / galactose kit was used to measure the content of lactose and galactose in the goat milk before and after the enzymatic hydrolysis of Example 2. It was detected that the lactose content in the functional prebiotic liquid goat milk prepared in E...

Embodiment 3

[0037] Step 1. Add lactase K and lactase P in a volume ratio of 2:1 to the goat milk that is pasteurized at 65°C for 15 minutes and cooled to 50°C, so that the final lactase content is 0.7 g / L;

[0038] Step 2: Add γ-aminobutyric acid and N-acetylneuraminic acid to make the final concentrations 2g / L and 1g / L respectively, stir evenly, and then enzymolysis at a constant temperature of 50°C for 2.5h;

[0039] Step 3. Add Baofeng seed oil and phytosterol to make the concentrations 3g / L and 5g / L respectively, homogenize under high pressure for 10min, keep at 132°C for 4s after sterilization, and pack to obtain functional low-lactose prebiotic liquid goat milk. The prepared functional prebiotic liquid goat milk has a shelf life of 60 days at room temperature.

[0040] The lactose / galactose kit was used to measure the content of lactose and galactose in the goat milk before and after the enzymatic hydrolysis of Example 3. It was detected that the lactose content in the functional pr...

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Abstract

The preparation method comprises the following steps: adding lactase K and lactase P into pasteurized goat milk cooled to 50 DEG C, then adding gamma-aminobutyric acid and N-acetylneuraminic acid until the final concentrations are 2g / L and 1g / L respectively, uniformly stirring, then carrying out constant-temperature enzymolysis, then adding asarum seed oil and phytosterol, uniformly stirring, and finally, carrying out constant-temperature enzymolysis, so as to obtain the functional low-lactose prebiotic liquid goat milk. The final concentrations are respectively 3g / L and 5g / L, homogenizing at high pressure for 10 minutes, sterilizing and packaging to obtain the functional low-lactose prebiotic liquid goat milk. The goat milk is used as a raw material, lactose in the goat milk is hydrolyzed into galactose and glucose under the combined action of two lactases, and the galactose and the glucose are converted into galactooligosaccharide through the transglycosylation of the lactases, so that the content of the lactose in the liquid milk is reduced, lactose intolerance is relieved, and the milk quality is improved. Meanwhile, the health-care food has the functions of promoting sleep, improving memory, resisting senile dementia and improving heart and blood vessels, and is suitable for middle-aged and elderly consumers.

Description

technical field [0001] The invention belongs to the technical field of dairy products, relates to functional dairy products, and in particular relates to a functional low-lactose prebiotic liquid goat milk and a preparation method thereof. Background technique [0002] Lactose intolerance (LI) refers to the fact that lactose cannot be decomposed in the small intestine due to the lack or insufficiency of lactase in the small intestine mucosa in the human body. After reaching the large intestine, it is decomposed by the intestinal flora to generate short-chain fatty acids and some gas, resulting in abdominal pain. , flatulence, and nausea. Worldwide lactose intolerance affects more than 50% of the population in South America, Africa and Asia, and almost 100% in some Asian countries. At present, there are four main methods for reducing lactose in milk, namely enzymatic hydrolysis, genetic engineering technology, physical removal and chemical acid hydrolysis. The enzymatic hyd...

Claims

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Application Information

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IPC IPC(8): A23C9/152A23C9/12A23C9/156
CPCA23C9/152A23C9/1206A23C9/156A23C9/1528
Inventor 舒国伟许运利代春吉殷春陈立郭梦徐笠豪陈合
Owner SHAANXI UNIV OF SCI & TECH
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