On-chip microgroove array digital PCR chip based on electrical impedance detection and manufacturing method thereof

Abstract

The invention discloses an on-chip microgroove array digital PCR chip based on electrical impedance detection. The on-chip microgroove array digital PCR chip comprises a microgroove array, a power supply module, a clock generation module, a digital control logic module, a driving module, an analog-to-digital converter, a backscattering module and a power-on reset module. The wireless power supply module generates standard voltage and current, the clock generation module generates clocks and impedance test excitation required by the digital module and the analog-to-digital converter, the power-on reset module generates reset signals for the digital circuit, and the backscattering module completes wireless transmission of ADC output signals to an upper computer. The digital control logic completes sequential gating and time division measurement of the array units. The system realizes full integration of a detection unit and a chip, does not need an external test board and complex connection, adopts an electrical impedance test principle, and abandons preposed steps and expensive instruments required by fluorescent PCR. The method provided by the invention can realize accurate analysis of PCR, and has the advantages of simplicity, portability and low cost.

Description

technical field [0001] The invention belongs to the field of circuit technology, in particular to an on-chip microgroove array digital PCR chip based on electrical impedance detection and a manufacturing method. Background technique [0002] As the most basic and extensive technology in molecular diagnosis, PCR is of great significance in medical diagnosis, gene replication, criminal evidence collection, archaeology and other application fields. Among them, the third-generation technology digital PCR, by realizing the differentiation and amplification of thousands of microchambers and the detection of a single microchamber, obtains digital 0 / 1 data, and calculates the PCR reaction result based on this, realizing a new, accurate, Efficient technology for absolute nucleic acid quantification. [0003] With the development of integrated circuits and related biosensor technology, digital PCR can be integrated in a small chip and perform specific functions. Gene chips integrate...

AI Technical Summary

Problems solved by technology

Existing traditional fluorescent dPCR instruments often do not have the above advantages, and cannot meet the clinical use or the purpose of diagnosis and treatment anytime and anywhere

However, the existing electrochemical detection of amplicons usually uses voltammetry to measure sample impedance. In order to enhance the signal to be detected, a pre-labeling process is usually used, which leads to additional intercalation agents or additional immobilization pretreatment. to improve the signal-to-noise ratio

Compared with the voltammetry method, the EIS system can effectively solve the above problems and defects by measuring the complex impedance of the sample at different frequencies, but the relevant research and system have not done the sample electrode contact experiment, or only measured the DNA Concentration versus frequency-domain impedance without real-time detection by PCR

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