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Collagen tripeptide product as well as preparation method and application thereof

A collagen tripeptide and product technology, applied in cosmetic preparations, peptides, cosmetics, etc., can solve the problems of poor anti-glycation effect of collagen peptides, and achieve a significant anti-sugar effect.

Active Publication Date: 2022-06-17
安徽盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of collagen tripeptide products rich in basic amino acids in the market, and the anti-glycation effect of collagen peptides is poor

Method used

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  • Collagen tripeptide product as well as preparation method and application thereof
  • Collagen tripeptide product as well as preparation method and application thereof
  • Collagen tripeptide product as well as preparation method and application thereof

Examples

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preparation example Construction

[0018] In order to facilitate obtaining more basic amino acids, preferably, the preparation method of the collagen tripeptide product comprises the following steps:

[0019] 1) Add water to the collagen to adjust the pH of the collagen solution to 5-8, preferably pH 6-7; add 0.5-5% composite protease by weight of collagen, preferably 1-4% composite protease, More preferably, it is 2-4% composite protease; under the condition of temperature of 45-60 ° C, enzymatic hydrolysis is carried out for 2-6 hours, preferably for 3-5 hours, to obtain an enzymatic hydrolysis solution; wherein, the composite protease includes figs protease and ginger protease, and the weight ratio of the ficin protease and the ginger protease is 2-4:1;

[0020] 2) adjusting the pH value of the enzymatic hydrolysis solution to 7-8.5, adding a starter of 1-8% by weight of collagen, preferably a starter of 3-6%; fermenting at 30-42° C. for 2-4 hours to obtain a fermentation broth; The starter comprises Debary...

Embodiment 1

[0049] Embodiment 1: a kind of collagen tripeptide rich in basic amino acids and preparation thereof

[0050] Step S1: Weigh 100 g of tilapia skin and cut it into small pieces, add 2000 mL of 0.8% sodium hydroxide solution to soak for 4 hours, wash 3 times, add demineralized water, and homogenize in a grinder to a paste, Preparation of total protein crude extract;

[0051] Step S2: adjusting the pH value of the crude extract of total protein to 2.6, heating to 90°C, and thermally extracting the gel for 5 hours to obtain crude collagen extract;

[0052] Step S3: Weigh 1 g of egg egg white, 3 g of sodium caseinate, and 4 g of okra polysaccharide, add it to the crude collagen extract, adjust the pH value to 3, stir evenly, and let stand for 4 hours, collect the supernatant, and obtain purified collagen thing;

[0053] Step S4: adjusting the pH value of the refined collagen product to 7.5 and the temperature to 55°C, adding 3 g of ficin protease and 1 g of ginger protease, and t...

Embodiment 2

[0055] Example 2: A collagen tripeptide rich in basic amino acids and its preparation

[0056] Step S1: Weigh 100 g of tilapia skin and cut it into small pieces, add 2000 mL of 0.8% sodium hydroxide solution to soak for 4 hours, wash 3 times, add demineralized water, and homogenize in a grinder to a paste, Preparation of total protein crude extract;

[0057] Step S2: adjusting the pH value of the crude extract of total protein to 2.6, heating to 90°C, and thermally extracting the gel for 5 hours to obtain crude collagen extract;

[0058] Step S3: Weigh 0.5g egg white, 0.5g sodium caseinate, 2g okra polysaccharide, add to the crude collagen extract, adjust the pH value to 3, stir evenly and let stand for 4 hours, collect the supernatant, and obtain collagen refined matter;

[0059] Step S4: adjust the pH value of the refined collagen product to 7.5, the temperature is 55°C, add 3 g of ficin protease and 1 g of ginger protease, and the enzymatic hydrolysis time is 6 h to obtai...

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Abstract

The invention provides a collagen tripeptide product, the collagen tripeptide contains tripeptide of which at least one end is alkaline amino acid, the molecular formula of the tripeptide of the alkaline amino acid comprises Gly-X-Arg, Gly-X-His and Gly-X-Lys, X is amino acid; based on the total weight of the collagen tripeptide, the content of the basic amino acid is not less than 10%. The invention also provides a method for preparing the collagen tripeptide, which comprises the following steps: adding water into the collagen to ensure that the pH value of the solution is 5-8, and adding the compound protease which is 0.5-5% of the weight of the collagen; carrying out enzymolysis for 2-6 hours at 45-60 DEG C; the compound protease comprises ficin protease and ginger protease; adjusting the pH value of the enzymatic hydrolysate to 7-8.5, adding a leavening agent accounting for 1-8% of the weight of the collagen, and fermenting at 30-42 DEG C for 2-4 hours; the leavening agent is prepared from debaryomyces hansenii, pediococcus pentosaceus and micrococcus mutans; and carrying out solid-liquid separation on the fermentation liquid, and collecting filtrate.

Description

technical field [0001] The invention relates to a collagen tripeptide product, a preparation method of the collagen tripeptide product and the application of the collagen tripeptide product in cosmetics. Background technique [0002] Collagen is an important component of extracellular matrix, and its content is about 1 / 3 of human protein, which plays an important role in maintaining the structure and function of the body. Collagen is lost as people age. At the same time, due to the low reversibility of collagen in the extracellular matrix of the dermis of skin tissue, it is more likely to undergo non-enzymatic reactions with metabolic molecules such as glucose to generate advanced glycation end products (AGE), such as glycosylated collagen, etc. A series of adverse effects on the organization. [0003] Collagen peptide is a small molecule peptide product made by enzymatic hydrolysis of macromolecular collagen. According to research reports, collagen peptides can improve s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/083C07K14/78C12P21/06A61K8/65A61Q19/00A23L33/18C12R1/645C12R1/01C12R1/225C12R1/44C12R1/265
CPCC07K5/0806C07K14/78C12P21/06A61K8/65A61Q19/00A23L33/18A61K2800/85A23V2002/00A23V2250/55
Inventor 王海燕李志国许智敏刘爱青刘利格宋萃
Owner 安徽盛美诺生物技术有限公司
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