Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting chlorogenic acid substances in plants based on SERS (Surface Enhanced Raman Scattering)

A technology for acids and substances, which is applied in the field of chlorogenic acid substances in plants based on SERS detection, can solve problems such as lack of enhanced Raman spectroscopy, and achieve the effects of simple and fast detection process, small deviation of detection results, and low cost.

Pending Publication Date: 2022-06-21
CHINA AGRI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the detection of CGAs content by enhanced Raman spectroscopy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting chlorogenic acid substances in plants based on SERS (Surface Enhanced Raman Scattering)
  • Method for detecting chlorogenic acid substances in plants based on SERS (Surface Enhanced Raman Scattering)
  • Method for detecting chlorogenic acid substances in plants based on SERS (Surface Enhanced Raman Scattering)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the content detection of chlorogenic acids in stevia

[0032] 1)Cu 2 Preparation of O-Ag SERS Substrates

[0033] The copper sheet (thickness is about 0.3mm) was polished with 7000-mesh polishing paper, cut into 1.5cm×1.5cm blocks, ultrasonically cleaned in acetone, ethanol and water for 10min in turn, taken out and put into a 50mL centrifuge tube, added The etching solution (9mL of water, 4mL of 10mol / L NaOH, 1mL of 1mol / L ammonium persulfate), reacted at room temperature for about 120min to generate copper oxide (CuO), took out and washed 4 times with deionized water and ethanol, and put it into a tube after drying Type Furnace, N 2 Sintering at 550℃ for 5h in the atmosphere produces cuprous oxide (Cu 2 O), take out after cooling to room temperature. the Cu 2 O put it into a 50mL centrifuge tube, add 20mL5×10 -4 mol / L silver nitrate aqueous solution (containing 2.5×10 -4 mol / L nitric acid) for 60 s, during which time it was shaken to generate Cu ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for detecting chlorogenic acid substances in plants based on SERS (Surface Enhanced Raman Scattering). The method comprises the following steps: S1, crushing a to-be-detected sample, extracting to obtain an extracting solution, and taking filtrate passing through a micro-filtration membrane as a to-be-detected solution; s2, dropwise adding the diluted to-be-detected liquid onto a surface-enhanced Raman substrate for Raman spectrum detection to obtain a signal of a Raman characteristic peak of the chlorogenic acid substances in the to-be-detected liquid; s3, preparing a standard solution of the chlorogenic acids, then dropwise adding the standard solution to the surface-enhanced Raman substrate, and carrying out Raman spectrum detection to obtain a signal of a Raman characteristic peak of the chlorogenic acids in the standard solution so as to obtain a standard curve between the concentration of the chlorogenic acids in the standard solution and the signal of the Raman characteristic peak of the chlorogenic acids in the standard solution; and detecting the chlorogenic acids in the sample to be detected according to the standard curve and a signal of the Raman characteristic peak of the chlorogenic acids in the liquid to be detected. The method is suitable for detecting the content of chlorogenic acid substances in plants such as eucommia ulmoides, stevia rebaudiana and honeysuckle.

Description

technical field [0001] The invention relates to a method for detecting chlorogenic acids in plants, in particular to a method for detecting chlorogenic acids in plants based on SERS. Background technique [0002] Chlorogenic acids (CGAs) have a variety of biological properties, including antibacterial and antiviral, anticancer, anti-inflammatory, and antioxidant properties, and also have hypoglycemic and hypolipidemic effects. In recent years, CGAs have been widely used, especially in the fields of pharmacology, chemical industry, food industry, cosmetic industry and so on. The content of CGAs in Eucommia ulmoides, honeysuckle, stevia and other plants is relatively high. At present, the commonly used detection methods include high performance liquid chromatography, reversed-phase chromatography, liquid chromatography-mass spectrometry, electrochemical method, and spectrophotometry. The HPLC method requires a variety of CGA standards for qualitative or quantitative analysis,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/65B82Y40/00B82Y15/00
CPCG01N21/658B82Y15/00B82Y40/00
Inventor 杨增玲刘二伟黄圆萍廖科科
Owner CHINA AGRI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com