Efficient preparation method of D-pantoic acid

A pantothenic acid, high-efficiency technology, applied in the field of biosynthesis, can solve the problems of limiting industrial application prospects, complicated reaction steps, long reaction time, etc., to protect the environment and the safety of production workers, avoid cumbersome steps, and simple preparation process Effect

Inactive Publication Date: 2022-06-24
ANHUI HUAHENG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are defects such as cumbersome reaction steps, long reaction time, and high cost caused by the consumption of the coenzyme NADPH in the process of converting ketopantoate into pantoate, which limits its industrial application prospects.

Method used

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  • Efficient preparation method of D-pantoic acid
  • Efficient preparation method of D-pantoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050]1) in the reaction vessel, add valine, methyl alcohol, compound enzyme, add water to total volume and be 5L, stir and dissolve, make in the reaction system: valine 249.5g, methyl alcohol 68.25g, L-amino acid deaminase is 10U / L, methanol dehydrogenase was 10U / L, aldolase was 15U / L, ketopantoate reductase was 13U / L, and formate dehydrogenase was 12U / L. Ammonium formate was added in a stream to maintain the concentration of ammonium formate in the reaction solution at 2 g / L. The temperature of the solution was adjusted to 37°C, the pH of the solution was adjusted to 5-7 with ammonia water and dilute sulfuric acid, and the reaction was continued for 20 hours under stirring conditions to obtain a D-pantoic acid solution. figure 1 .

[0051] 2) Take the D-pantoic acid reaction solution, pass it through a ceramic membrane, collect the clear phase solution, pass through a nanofiltration membrane, and adjust the collected clear solution to pH 2.0 or less with sulfuric acid, and...

Embodiment 2

[0053] 1) in the reaction vessel, add valine, methyl alcohol, compound enzyme, add water to total volume and be 5L, stir and dissolve, make in the reaction system: valine 249.5g, methyl alcohol 68.25g, L-amino acid deaminase is 10U / L, methanol dehydrogenase is 10U / L, aldolase is 18U / L, ketopantoate reductase is 14U / L, and formate dehydrogenase is 12U / L. Ammonium formate was added in a stream to maintain the concentration of ammonium formate in the reaction solution at 2 g / L. The temperature of the solution was adjusted to 37°C, the pH of the solution was adjusted to 5-7 with ammonia water and dilute sulfuric acid, and the reaction was continued for 20 hours under stirring conditions to obtain a D-pantoic acid solution. figure 1 .

[0054] 2) Take the D-pantoic acid reaction solution, pass it through a ceramic membrane, collect the clear phase solution, pass through a nanofiltration membrane, and adjust the collected clear solution to pH 2.0 or less with sulfuric acid, and st...

Embodiment 3

[0056] 1) in the reaction vessel, add valine, methyl alcohol, compound enzyme, add water to total volume and be 5L, stir and dissolve, make in the reaction system: valine 300g, methyl alcohol 85g, L-amino acid deaminase are 10U / L, Methanol dehydrogenase was 10U / L, aldolase was 18U / L, ketopantoate reductase was 14U / L, and formate dehydrogenase was 12U / L. Ammonium formate was added in a stream to maintain the concentration of ammonium formate in the reaction solution at 2 g / L. The temperature of the solution was adjusted to 37°C, the pH of the solution was adjusted to 5-7 with ammonia water and dilute sulfuric acid, and the reaction was continued for 20 hours under stirring conditions to obtain a D-pantoic acid solution. figure 1 .

[0057] 2) Take the D-pantoic acid reaction solution, pass it through a ceramic membrane, collect the clear phase solution, pass through a nanofiltration membrane, and adjust the collected clear solution to pH 2.0 or less with sulfuric acid, and sti...

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Abstract

The invention relates to an efficient preparation method of D-pantoic acid, which specifically comprises the following steps: (1) valine, methanol and compound enzyme are mixed with water, and the compound enzyme comprises L-amino acid deaminase, methanol dehydrogenase, aldolase, formate dehydrogenase and ketopantoic acid reductase; (2) ammonium formate is added, and D-pantoic acid is prepared through an enzyme catalytic reaction under the conditions that the temperature is 25-55 DEG C and the pH value is 4-7; wherein the molar ratio of the valine to the methanol is (0.9 to 1.1): (0.9 to 1.1). According to the method, valine and methanol are used as substrates for fermentation and conversion to generate D-pantoic acid, the raw materials are cheap and easy to obtain, and the reaction cost is low.

Description

technical field [0001] The invention relates to the field of biosynthesis, in particular to an efficient preparation method of D-pantoate. Background technique [0002] Pantothenic acid also known as vitamin B 5 , is an essential nutrient element for mammals including humans and livestock, and is used in the biosynthesis of coenzyme A (CoA) and acyl carrier protein (ACP) in body cells, and then participates in more than one hundred cellular metabolic reactions. [0003] D-pantolactone is an important precursor for the synthesis of D-pantothenic acid. CN110423717A discloses a preparation method of D-pantoic acid lactone, wherein DL-pantoic acid lactone is selectively hydrolyzed and separated by enzymatic method or synthesized by enzymatic method to prepare D-pantoic acid lactone. However, there are the following defects. The synthesis of DL-pantolactone requires the use of a large amount of isobutyraldehyde and formaldehyde. Formaldehyde is irritating to the human body, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C07D307/33
CPCC12P7/42C07D307/33
Inventor 周芳芳刘树蓬刘磊
Owner ANHUI HUAHENG BIOTECH
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