Eucalyptol type sesquiterpenoids in ragweed as well as preparation method and application of euptol type sesquiterpenoids

A eucalyptane-type and sesquiterpenoid technology, applied in the agricultural field, can solve problems such as unclear chemical substance basis, and achieve the effect of reducing input and harm to the environment

Active Publication Date: 2022-06-28
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above further confirms that allelopathy is an important reason for the successful invasion of ragweed, but most of the relevant studies only consider the effects of its extracts on local companion plants, and the chemical basis of its allelopathy is still unclear

Method used

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  • Eucalyptol type sesquiterpenoids in ragweed as well as preparation method and application of euptol type sesquiterpenoids
  • Eucalyptol type sesquiterpenoids in ragweed as well as preparation method and application of euptol type sesquiterpenoids
  • Eucalyptol type sesquiterpenoids in ragweed as well as preparation method and application of euptol type sesquiterpenoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of eucalyptane-type sesquiterpenoids in ragweed

[0048] (1) get 50kg of dried ragweed upper part and extract 3 times with 80% ethanol cold soaking, every 7 days, extract 1.2kg of extract after being concentrated by rotary evaporator;

[0049] (2) extract 3 times with 5L petroleum ether and 5L ethyl acetate successively after the extract is suspended with water, and the ethyl acetate extract is concentrated by rotary evaporator to obtain 230g of ethyl acetate extract;

[0050] (3) The ethyl acetate extract was separated by silica gel column chromatography, eluted with a gradient of dichloromethane / methanol system, and the volume ratios were 99:1, 98:2, 95:5, 90:10, 80:20 , 70:30, each 800mL is a fraction, a total of 42 fractions were collected, and after TLC detection and analysis, five fractions of Fr.A~Fr.E were obtained, of which 18g of Fr.D was obtained;

[0051] (4) The Fr.D part was separated by MCI column chromatography and eluted with a me...

Embodiment 2

[0054] The preparation of eucalyptane-type sesquiterpenoids in ragweed

[0055] (1) get 40kg of dried ragweed upper part and extract 3 times with 80% ethanol cold soaking, every 7 days, extracting solution obtains extract 0.9kg after rotary evaporator concentration;

[0056] (2) extract 3 times with 5L petroleum ether and 5L ethyl acetate successively after the extract is suspended with water, and the ethyl acetate extract is concentrated by rotary evaporator to obtain 210g of ethyl acetate extract;

[0057] (3) The ethyl acetate extract was separated by silica gel column chromatography, eluted with a gradient of dichloromethane / methanol system, and the volume ratios were 99:1, 95:5, 90:10, 80:20, 70:30 , 50:50, each 800mL is a fraction, a total of 38 fractions were collected, and after TLC detection and analysis, five fractions of Fr.A~Fr.E were obtained, of which 14g of Fr.D was obtained;

[0058] (4) The Fr.D part was separated by MCI column chromatography and eluted with ...

Embodiment 3

[0061] Example 3 Preparation of eucalyptane-type sesquiterpenoids in ragweed

[0062] (1) get 60kg of dried ragweed upper part and extract 3 times with 80% ethanol cold soaking, every 7 days, extracting solution obtains extract 1.5kg after rotary evaporator concentrating;

[0063] (2) extract 3 times with 5L petroleum ether and 5L ethyl acetate successively after the extract is suspended with water, and the ethyl acetate extract is concentrated by rotary evaporator to obtain 280g of ethyl acetate extract;

[0064] (3) The ethyl acetate extract was separated by silica gel column chromatography, eluted with a gradient of dichloromethane / methanol system, and the volume ratios were 98:2, 95:5, 90:10, 80:20, 70:30 , 50:50, each 800mL is a fraction, a total of 50 fractions are collected, and after TLC detection and analysis, five fractions of Fr.A~Fr.E are combined to obtain 23g of Fr.D fraction;

[0065] (4) The Fr.D part was separated by MCI column chromatography and eluted with ...

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Abstract

The invention belongs to the technical field of agriculture, and particularly relates to a cineole type sesquiterpenoids compound in ragweed as well as a preparation method and application of the cineole type sesquiterpenoids compound. The preparation method comprises the following steps: drying the aboveground part of ragweed, extracting with ethanol, and concentrating the extracting solution with a rotary evaporator to obtain extract; suspending the extract with water, and sequentially extracting with petroleum ether and ethyl acetate; carrying out silica gel column chromatography separation on the ethyl acetate extract to obtain five parts of Fr.A-Fr.E; the Fr.D part is subjected to MCI column chromatography separation, and five parts Fr.D-1-Fr.D-5 are obtained; carrying out gel column chromatography separation on the Fr.D-2 part to obtain eight parts of Fr.D-2-1 to Fr.D-2-8; and separating the Fr.D-2-3 part by adopting HPLC (High Performance Liquid Chromatography) to obtain a target monomeric The preparation method disclosed by the invention is simple and good in reproducibility, and the prepared compound is high in purity and has different degrees of growth inhibition effects on common weeds.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a eucalyptane-type sesquiterpenoid compound in ragweed and a preparation method and application thereof. Background technique [0002] In recent years, with the acceleration of economic globalization and international trade liberalization, especially the rapid development of tourism, the invasion and spread of alien plants has become faster and faster. constitutes a serious threat. An important reason for the successful invasion of exotic plants is that they release secondary metabolites that are relatively novel compared to native plants in the invasion area, and are released into the environment through suitable pathways such as gas volatilization, rain leaching, root exudation, and stubble degradation. It affects the growth and development of native associated plants, so that alien plants have an advantage in the process of interaction with native plants, and ...

Claims

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Application Information

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IPC IPC(8): C07D307/92A01N43/12A01P13/00
CPCC07D307/92A01N43/12C07B2200/07Y02A50/30Y02A90/40
Inventor刘志翔安桐冯玉龙曲波张彤
OwnerSHENYANG AGRI UNIV