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Lactobacillus rhamnosus strain LRa90, application of lactobacillus rhamnosus strain LRa90 in preparation of products for treating allergy and/or inhibiting pathogenic bacteria and products

A technology of Lactobacillus rhamnosus and pathogenic bacteria, which is applied in the field of microorganisms and can solve the problems of limited available strains

Pending Publication Date: 2022-06-28
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wherein Lactobacillus rhamnosus has fewer reports on direct treatment of allergies, and the existing available bacterial strains are quite limited. This area urgently needs to develop more new bacterial strains of Lactobacillus rhamnosus that can be used to relieve allergies

Method used

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  • Lactobacillus rhamnosus strain LRa90, application of lactobacillus rhamnosus strain LRa90 in preparation of products for treating allergy and/or inhibiting pathogenic bacteria and products
  • Lactobacillus rhamnosus strain LRa90, application of lactobacillus rhamnosus strain LRa90 in preparation of products for treating allergy and/or inhibiting pathogenic bacteria and products
  • Lactobacillus rhamnosus strain LRa90, application of lactobacillus rhamnosus strain LRa90 in preparation of products for treating allergy and/or inhibiting pathogenic bacteria and products

Examples

Experimental program
Comparison scheme
Effect test

experiment example 4

[0046] Escherichia coli, Salmonella, and Staphylococcus aureus used in Experimental Example 4 were purchased from Guangdong Microorganism Culture Collection;

experiment example 5

[0047] The HT-29 cells used in Experimental Example 5 were purchased from the China Type Culture Collection;

[0048] The LGG strains used in experimental examples 2 and 5 were purchased from the China Type Culture Collection;

[0049] The mice used in Experimental Examples 6 and 7 were purchased from Hunan Slike Jingda Laboratory Animal Co., Ltd.

[0050] The first group of examples, the strain LRa90 of the present invention

[0051] The examples in this group provide a strain of Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus) strain LRa90, its deposit number is CCTCC NO: M 2022573.

[0052] Any Lactobacillus rhamnosus (Lactobacillus rhamnosus ( Lacticaseibacillus rhamnosus ) behavior of strain LRa90 all fall into the protection scope of the present invention.

[0053] According to the teaching and inspiration of the present invention, for actual production needs, those skilled in the art select suitable adjuvants to be prepared in combination with the technical m...

experiment example 1

[0078] Experimental example 1. Bacterial isolation and identification

[0079] (1) Separation and screening:

[0080] Fecal samples were collected from people in remote pastoral areas in China, diluted with sterile saline to an appropriate gradient, spread on MRS agar plates, and incubated at 37°C for 48h. Opaque milky white, round and glossy monoclonal colonies with neat edges, raised and moist surfaces were selected, and purified and cultured by repeated streaking on MRS solid medium. Individual morphology was observed by optical microscope to obtain Lactobacillus strains.

[0081] MRS medium ingredients: peptone 10g, beef dipping powder 5g, yeast dipping powder 4g, K 2 HPO 4 2g, Triammonium Citrate 2g, Sodium Acetate 5g, Glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.58g, MnSO4 4H 2 O 0.25g, distilled water 1000mL; pH value 6.2±0.2

[0082] (2) Molecular biological identification:

[0083] The genomic DNA of the Lactobacillus strain was extracted with a genomic DNA ext...

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Abstract

The invention discloses a lactobacillus rhamnosus strain LRa90, application of the lactobacillus rhamnosus strain LRa90 in preparation of products for treating allergy and / or inhibiting pathogenic bacteria and the products, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus strain LRa90 is CCTCC (China Center for Type Culture Collection) NO: M 2022573. The invention further provides an application of the lactobacillus rhamnosus strain LRa90 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2022573 in the aspect of preparing a product for treating allergy and / or inhibiting pathogenic bacteria. Based on the lactobacillus rhamnosus LRa90, the invention also provides a product for treating allergy and a product for inhibiting pathogenic bacteria. The lactobacillus rhamnosus LRa90 is safe and free of pathogenicity, can be widely applied to the field of food, and can achieve the purpose of treating and relieving allergy through daily intake.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Lactobacillus rhamnosus strain LRa90 and its use and products for preparing products for treating allergies and / or inhibiting pathogenic bacteria. Background technique [0002] Allergy, also known as atopic reaction or allergic reaction, refers to the abnormal tendency to produce specificity to harmless and ubiquitous environmental allergens, which may occur in the intestines, skin and respiratory tract, mainly including abdominal pain, diarrhea, atopic dermatitis, changes allergic rhinitis, asthma and other clinical manifestations. Milk and eggs are high-quality protein foods, rich in nutrients, and the most important food and nutrition sources for infants and children, but also the most common protein allergens. β-lactoglobulin in milk and ovalbumin in eggs are the main allergens that cause allergies in infants and children. It can induce type I hypersensit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P37/08A23L33/135A23C9/123C12R1/225
CPCA61K35/747A61P31/04A61P37/08A23L33/135A23C9/1234A23V2002/00A23V2400/175A23V2200/304
Inventor 方曙光刘欢朱明明孔素芬宋沙沙陈明阳
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD