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Device for culturing algae at constant temperature and experimental method

A constant temperature culture and algae technology, applied in the direction of microorganism-based methods, single-cell algae, biochemical cleaning equipment, etc., can solve the problems of difficult control of temperature conditions, inapplicable algae growth conditions, and the incubator temperature cannot be directly observed, etc., to achieve Uniform heating, reducing the entry of air pollution particles, and the effect of even heating of algae

Pending Publication Date: 2022-07-05
ANHUI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture method uses pipelines and relies on power to make the culture fluid flow and introduce carbon dioxide-containing air, but the equipment is complicated
Biological contamination, such as bacterial growth, is prone to occur in open culture cultures
[0005] At present, most of the devices for cultivating algae adopt the method of incubator cultivation. The incubator is a closed and opaque structure, which is not suitable for directly observing the growth of algae. It is also necessary to replace the air pump regularly to maintain sufficient carbon sources inside the algae. At the same time, the incubator The medium temperature cannot be directly observed, and the temperature conditions are difficult to control, resulting in the failure of algae cultivation. Therefore, a cultivation device with controllable temperature, simple equipment and low energy consumption is needed

Method used

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  • Device for culturing algae at constant temperature and experimental method
  • Device for culturing algae at constant temperature and experimental method
  • Device for culturing algae at constant temperature and experimental method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A device for culturing algae at a constant temperature, comprising a pool 1 and a transparent culture cylinder 5, a heating rod 2 and a temperature sensor 13 are installed inside the pool 1, a water pump 3 is installed outside the pool 1, and the water pump 3 The output end of the water inlet pipe 10 is connected and fixed, and the water inlet pipe 10 is connected and fixed to the transparent culture cylinder 5;

[0039] The transparent culture cylinder 5 is a double-layered cylindrical structure and is provided with two. The lamp tube 15 and the aeration head 12 are installed inside the transparent culture cylinder 5, and the air inlet pipes 14 and 12 are arranged outside the transparent culture cylinder 5. Drainage pipe 16 and water outlet 6; temperature sensor 13 and heating rod 2 are provided inside the pool 1 and a water pump 3 is provided outside the pool 1, and the water pump 3 inputs water into the transparent culture cylinder 5 interlayer through the water inlet...

Embodiment 2

[0051] On the basis of Example 1, see Figure 4-9 , Embodiment 2 provided by the present invention, the experimental method of the device for constant temperature cultivation of algae, includes the following steps:

[0052] Step 1: Weigh 1.7g of BG-11 medium, dissolve it in 1L of pure water, and sterilize it at 121°C for 25min to obtain an algal culture solution;

[0053] Step 2: Pour tap water into the pool, submerge the temperature sensor, turn on the main control box at the same time, control the water temperature of the pool at 20°C~30°C, open the valve on the pumping pipe, turn on the power of the pumping water pump, and input the pool water into the transparent In the interlayer of the culture tube 5, turn on the lamp switch at the same time, and control the illumination time for 12 hours through the timing device, and the illumination intensity is 5000-10000Lux;

[0054] Step 3: Close the shut-off valve under the acrylic plate column, close the water outlet, pour 5L of...

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Abstract

The invention discloses a device for culturing algae at constant temperature and an experimental method, the device comprises a water tank, a transparent culture cylinder, water inlet and outlet equipment, a heating, lighting and air supply structure and the like, and the experimental method comprises the steps 1-5. The culture device designed by the invention adopts a water bath temperature control design concept, and has the advantages of controllable temperature, stable heating and uniform algae heating; according to the transparent culture cylinder and the mounting structure thereof, entering of air pollution particles can be reduced; water used by the heat preservation layer can be recycled by water in the water pool; the main control box is adopted to control the illumination intensity, and the timing device is adopted to control the lamp tube to control the illumination time to achieve the optimal growth condition; by using the design concept of water bath temperature control, the device has the advantages of controllable temperature, stable heating and uniform heating of algae, and redundant water is discharged by the drainage pipeline above the acrylic plate column and enters the water pool again to realize water recycling.

Description

technical field [0001] The invention relates to the technical field of algae cultivation, in particular to a device and an experimental method for culturing algae at a constant temperature. Background technique [0002] Microalgae is a single-celled organism with a high oil content, especially Chlorella, the oil content can reach 20% of the dry cell weight, a variety of nutrients, can be used to prepare biodiesel, can be taken as a nutritional supplement, and Chlorella has the functions of anti-aging and beauty. Scenedesmus is rich in protein and vitamins, and is a good feed for fish. If you use organic sewage to oxidize fish, you can get high yield. The proliferating algae can also be used as feed for poultry. At the same time, microalgae have broad application prospects in feed, food, medicine and cosmetics. The basis of these applications is the large-scale cultivation of microalgae. [0003] During the cultivation process of algae, the growth and reproduction speed fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00C12M1/34C12M1/24C12M1/08C12M1/36C12N1/12C12R1/89
CPCC12M41/12C12M41/48C12M21/02C12M23/22C12M23/00C12M29/06C12M23/06C12M29/20C12M41/44C12M41/06C12N1/12
Inventor 文豪秦微尹鸿伟章浩文赵逸凡朱敏
Owner ANHUI UNIV OF SCI & TECH
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