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Micro-fluidic chip and application thereof

A microfluidic chip, chip technology, applied in laboratory containers, enzymology/microbiology devices, methods of supporting/immobilizing microorganisms, etc., can solve the problem of inability to remove cryoprotectants, weak removal ability, and cell recovery rate lower problem

Pending Publication Date: 2022-07-08
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing cryoprotectant removal methods (such as one-step or multi-step centrifugation, dialysis, diffusion, etc.) have weak removal ability, low cell recovery rate, and cannot remove cryoprotectant in trace cells.

Method used

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  • Micro-fluidic chip and application thereof
  • Micro-fluidic chip and application thereof
  • Micro-fluidic chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] has as figure 1 A microfluidic chip of the shown structure includes:

[0052] The microfluidic chip has a rectangular first chip 1 and a second chip 4 with a length, width and height of 4 cm, 2 cm and 0.5 cm respectively; the material of the first chip 1 and the second chip 4 is polydimethylsiloxane. The first lower surface of the first chip 1 includes a first channel 2, the depth of which is 40 μm, the width of the main part (ie the part of the shrink-like structure without the inlet and outlet) is 1 mm, and the length is 1.5 cm. An array of inclined trenches 13 is constructed in the first channel 2 on the first lower surface of the first chip 1. The width of the trenches 13 is 25 μm and the depth is 25 μm. The angle between the axial direction of the trench 13 and the long sidewall of the first channel 2 is 60°, the spacing between the grooves 13 is 25 μm, and the array of grooves 13 is filled with straight channel parts.

[0053] like figure 2 As shown, the first...

Embodiment 2

[0059] In the embodiment of the present invention, the first channel 2 is a flow cavity located on the first lower surface of the first chip 1 , and the second channel 5 is a flow cavity located on the second upper surface of the second chip 4 . The first channel 2 and the second channel 5 are similar in shape, and the flow chamber can be divided into inlet and outlet portions at both ends and a main body chamber portion in the middle. A layer of porous membrane 3 is sandwiched between the first chip 1 and the second chip 4 . The surfaces of the first chip 1 and the second chip 4 with channels are attached to each other. The first chip 1 and the second chip 4 are placed in parallel, and after the main body cavity parts are completely aligned and overlapped, they are staggered by a distance in the lateral and longitudinal directions respectively, so as to achieve the effect of the main body cavity parts being overlapped. The overlapping part of the main body cavity of the seco...

Embodiment 3

[0061] In the embodiment of the present invention, the depth of the first channel 2 is smaller than the thickness of the first chip 1 , and the depth of the second channel 5 is smaller than the thickness of the second chip 4 . The overlapping part of the first channel 2 and the second channel 5 is the dialysis zone 10, and its position and shape are as follows: figure 2 shown in.

[0062] figure 2 Among them, the first inlet 8 is the cleaning liquid inlet, and the first outlet 7 is the cleaning liquid outlet. In the embodiment of the present invention, the first channel 2 is a cleaning solution channel, and the isotonic cleaning solution without cryoprotectant enters the chip at a fixed flow rate from the first inlet 8 , and the second channel between the dialysis zone 10 and the second chip 4 The fluid in channel 5 is dialyzed. In the first channel 2 of the first chip 1, parallel, elongated (rectangular cross-section) protrusions are constructed, grooves 13 are formed be...

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Abstract

The invention relates to the field of micro-fluidic chips, in particular to a micro-fluidic chip and application thereof. The invention provides a micro-fluidic chip. The micro-fluidic chip comprises a first chip and a second chip, the first chip comprises a first upper surface and a first lower surface; the second chip comprises a second upper surface and a second lower surface; the first chip is provided with a first inlet and a first outlet; the second chip is provided with a second inlet and a second outlet; the first lower surface is provided with a first channel; the second upper surface is provided with a second channel; the first channel and the second channel are respectively provided with a groove array; the first channel and the second channel are oppositely attached; a porous membrane is arranged between the first channel and the second channel; and the first chip, the porous membrane and the second chip are sequentially arranged from top to bottom. The micro-fluidic chip provided by the invention is realized based on a flow focusing-membrane separation technology, can continuously, efficiently and safely remove a cell low-temperature protective agent, and can be applied to removal of magnetic nanoparticles in cytotoxin or extracellular fluid.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to microfluidic chips and applications thereof. Background technique [0002] Cryopreservation technology refers to the technology of cryopreservation of organisms, which can significantly prolong the preservation time of biological samples, and has been widely used in the long-term preservation of various cell products (such as blood cells, stem cells, strains, etc.). During cryopreservation of cells, the precipitated ice crystals can cause damage to cells. In order to reduce cell damage during cryopreservation, a certain amount of cryoprotectant (such as dimethyl sulfoxide (DMSO), glycerol, polyethylene glycol, etc.) needs to be added to the cell suspension before the cells are cryopreserved. Cryoprotectants can reduce cell damage during cryopreservation, however, cryoprotectants are usually cytotoxic and can also cause cell penetrating damage. Therefore, cryoprotectants need t...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/12C12M1/00
CPCB01L3/5027C12M47/04Y02A50/30
Inventor 丁卫平王港国李士博李成盼
Owner UNIV OF SCI & TECH OF CHINA
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