Method for measuring osmotic pressure of culture solution in cell culture and biochemical device

Abstract

The invention provides a method for measuring the osmotic pressure of a culture solution in cell culture and a biochemical device.The method for measuring the osmotic pressure of the culture solution in cell culture comprises the following steps that a culture solution sample is taken, and the concentration of organic matter in the culture solution sample is measured; measuring the concentration of metal ions in the culture solution sample by adopting an ion selective electrode according to a Nernst equation; according to the concentration of the organic matters and the concentration of the metal ions, the osmotic pressure of the culture solution sample is calculated according to the following osmotic pressure formula: O osmotic pressure = C total organic matters + C total metal ions * K + b, O osmotic pressure represents the osmotic pressure of the culture solution sample, and the unit is mOsm / kg; the total organic matter C represents the total concentration of all organic matters in the culture solution sample, and the unit is mmol / L; the total C metal ions represent the total concentration of all metal ions in the culture solution sample, and the unit is mmol / L; k and b are constants. Interference caused by environment change is reduced, and measurement accuracy is improved.

Description

technical field [0001] The present application belongs to the technical field of solution osmotic pressure detection, and more particularly, relates to a method and a biochemical device for measuring the osmotic pressure of culture solution in cell culture. Background technique [0002] With the widespread use of antibodies in the treatment of cancer and autoimmune diseases, large-scale, high-density cell cultures are required to meet the growing market demand. Osmolality is an important process variable during mammalian cell culture. The osmotic pressure of the cell culture fluid is generally designed to be 260~320mOsm / kg. During the cell culture process, it is necessary to frequently measure whether the osmotic pressure of the cell culture fluid is isotonic with the cells. If the osmotic pressure is not isotonic, the osmotic pressure of the cell culture fluid will increase. At higher levels, the cells will rupture; at lower levels, the osmotic pressure of the cell culture...

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Problems solved by technology

[0004] Based on this, an object of this application is to provide a kind of assay method of culture fluid osmotic pressure in cell culture, to solve the method for measuring culture fluid osmotic pressure in the process of animal cell culture by freezing point osmometer existing in the prior art. There are strict requirements on temperature and humidity. Too high temperature and too low humidity may cause serious errors in measurement and affect the accuracy of measurement results Technical problems

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