Monomer emperin B extracted from golden chrysanthemum as well as extraction method and application of monomer emperin B
An extraction method and a technology of goldenrod chrysanthemum, applied in the field of traditional Chinese medicine, can solve the problems such as the antioxidative effect of goldenrod chrysanthemum has not been found, and achieve the effect of good application prospect and good antioxidant capacity.
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[0075] According to a particularly preferred embodiment of the present invention, the extraction method for the extraction of monomeric imperial chrysanthemum B, comprising the following steps:
[0076] (1) After drying and pulverizing the goldenrod chrysanthemum, it is mixed with an ethanol-water solution (the volume ratio of ethanol and water is 19:1) for 2-3 extractions, and in the process of each extraction, the extraction solvent I and the golden thread are extracted. The mass ratio of yellow chrysanthemum is 3-8:1, the time is 4-6 days, the extract is obtained after solid-liquid separation, and the extract is obtained after concentrating the extract;
[0077] (2) after adding the extraction extract obtained in step (1) to an appropriate amount of water to suspend, and then extracting with petroleum ether, ethyl acetate and n-butanol and then concentrating to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract , wherein, the mass ratio of petroleum...
Embodiment 1
[0084] (1) Take 9.9kg of golden silk chrysanthemum, after drying and pulverizing, extract twice with ethanol-water solution (the volume ratio of ethanol and water is 19:1), in the process of each extraction, ethanol-water-soluble and gold The mass ratio of the dry weight of silk yellow chrysanthemum is 5:1, and the extraction time is 5 days. After two extractions, filtration is carried out respectively, the filtrates are combined to obtain an extract, and the extract is concentrated under reduced pressure to obtain an extraction extract;
[0085] (2) adding an appropriate amount of water to the extraction extract obtained in step (1) to suspend, successively extracting with petroleum ether, ethyl acetate and n-butanol to obtain extracts of various parts, and then concentrating respectively to obtain petroleum ether extract, ethyl acetate Extract and n-butanol extract, wherein, the mass ratio of petroleum ether, ethyl acetate and n-butanol to extraction extract is 5:1;
[0086]...
Embodiment 2
[0108] Example 2 ABTS of compound JS-3-1-30 + Free radical scavenging activity screening
[0109] Using ABTS +Free radical scavenging method (ABTS kit) was used to evaluate the antioxidant capacity of compound JS-3-1-30 isolated from Chrysanthemum chinensis.
[0110] Compound JS-3-1-30 was prepared into solutions with concentrations of 1.0, 0.5, 0.2, 0.1, 0.05 mmol / L, respectively, and the positive control drug vitamin C (Vc) was prepared with concentrations of 1.0, 0.8, 0.6, 0.4, 0.2 mmol / L solution, 10 μL of each was added to the 96-well plate independently, and then 190 μL of ABTS was taken separately. + The solution was added to each well of a 96-well plate, and after mixing, let it stand in the dark for 6 minutes at room temperature, and then detect the absorbance (A) of each well of the 96-well plate at 405 nm, and calculate the free radical scavenging rate and IC. 50 value, the results are shown in Table 2,
[0111] Free radical scavenging rate = [A 空白 -(A 测定 -A ...
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