Application of corn protein peptide embedded fucoxanthin composite nanoparticles in preparation of weight-losing drink
A technology of composite nanoparticles and zein peptides, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and non-active ingredients in polymer compounds, can solve the problems of unseen cases and rare cases, and achieve remarkable results. Improve solubility and good stability
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Embodiment 1p
[0039] Example 1 pH value measurement
[0040] Take a 100ml sample, at room temperature, use a pH meter (brand: Mettler Shanghai), and measure it after calibration.
[0041] The results showed that the pH of the drink was between 5.8 and 8.0.
Embodiment 2
[0042] Example 2 Measurement of particle size, dynamic light scattering (PDI) and zeta potential
[0043] Measured by Malvern ZS90 at 25°C. Particle size and PDI data (Dynamic Light Scattering) were measured using a disposable plastic sample cell that transmits light on all sides. Zeta potential data were obtained with a Malvern Model 1070 Potentiometric Cell.
[0044] from figure 1 It can be seen that the DLS particle size of the zein peptide-embedded fucoxanthin composite nanoparticles is between 68nm and 350nm, the PDI value is between 0.15 and 0.25, and the zeta potential value is between -25 and -40. between. The particles are uniformly distributed and the surface has a negative charge.
Embodiment 3
[0045] Example 3 Detection of active substance parameters
[0046] Sample pretreatment:
[0047] Take 100ml of the drink, freeze and vacuum dry it at -40°C for 24 hours to obtain product powder A (containing FX-ZH) for later use. In order to extract the core substance FX and the wall material plant polypeptide ZH in A, accurately weigh 0.02g-0.04g (accurate to 0.001g) of A to be tested, add 1ml of water, put it in an ultrasonic cleaner for 5 minutes to make it fully dissolved . Add 1 mL of methyl tert-butyl ether / acetone (1:1, v / v) to 1 mL of freshly prepared aqueous solution and centrifuge at 4000 g for 10 minutes. At this time, all FX were dissolved in the organic phase, while the wall material of the plant polypeptide ZH was in the aqueous phase. Collect the organic phase containing FX and repeat the above steps 2-3 times until the organic phase is transparent. After separating the organic phase and the aqueous phase, the organic phase was dried under nitrogen flow to o...
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