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Preparation device of pathogenic microorganism transfer liquid and preparation method of transfer liquid

A pathogenic microorganism and preparation device technology, which is applied to the field of pathogenic microorganism transport liquid preparation device and transport liquid preparation, can solve the problems that the transport liquid is difficult to preserve pathogens, cannot be used for transport at the same time, and is inconvenient to detect pathogenic microorganisms, etc., and achieves preservation of RNA and DNA. Integrity, convenient nucleic acid detection, and the effect of reducing the risk of infection

Pending Publication Date: 2022-07-15
大能生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the pathogenic microorganism transport liquid in the prior art, which cannot be applied to the transfer of bacterial samples and virus samples at the same time, and the transfer liquid is difficult to preserve the activity of pathogens, which is not convenient for subsequent detection of pathogenic microorganisms. , and a preparation device for transporting liquid of pathogenic microorganisms and a preparation method of transporting liquid are proposed, the purpose of which is to prepare a pathogenic microorganism suitable for sampling bacterial samples and virus samples, which is convenient for subsequent detection of pathogens, as well as the cultivation and isolation of pathogens transport fluid

Method used

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  • Preparation device of pathogenic microorganism transfer liquid and preparation method of transfer liquid
  • Preparation device of pathogenic microorganism transfer liquid and preparation method of transfer liquid
  • Preparation device of pathogenic microorganism transfer liquid and preparation method of transfer liquid

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Embodiment 1

[0038] refer to Figure 1-3 , a pathogenic microorganism transport liquid preparation device, including an equipment compartment 10, a liquid mixing mechanism, a filling mechanism and a control terminal. The lower four corners of the plate 15 are provided with universal wheels, the top of one side of the nozzle 43 is provided with a placement table 11, and the other side is provided with a collection port 14, and the equipment compartment 10 is connected to the first. The two sides corresponding to the storage box 20 and the second storage box 30 are provided with liquid storage ports 12, and the side corresponding to the lower conveyor belt 60 is provided with a conveying port 13, and the lower port of the conveying port 13 is lower than the On the upper surface of the lower conveyor belt 60, its upper end surface is not higher than the lower surface of the upper conveyor belt 70, the upper surface of the upper conveyor belt 70 is located in the collection port 14, and the tw...

Embodiment 2

[0045] In this embodiment, the method for preparing the transport liquid is carried out by using the pathogenic microorganism transport liquid preparation device in the first embodiment, and the preparation method includes the following steps:

[0046] Step 1: First, inject the non-inactivated transport liquid into the first solution tank 25, inject the guanidine salt inactivator into the second solution tank 35, store it, and pass the filling tray 17 through the delivery port 13 on the equipment compartment 10 in turn. It is placed on the lower conveyor belt 60, and the first delivery pump 21 and the second delivery pump 31 are activated through the control terminal to inject the non-inactivated transport fluid in the first solution tank 25 and the guanidine salt inactivator in the second solution tank 35. In the stirring tank 50, the first flow meter 24 and the second flow meter 34 are used to monitor the liquid delivery volume in the first delivery pipe 23 and the second del...

Embodiment 3

[0052] In this embodiment, when using the pathogenic microorganism transport liquid preparation device to prepare the transport liquid, the second transport pump 31 and the second solenoid valve 28 are closed, the first transport pump 21 and the first solenoid valve 27 are opened, and the first solution The non-inactivated transport liquid in the tank 25 is injected into the solution bottle in the filling tray 17 through the nozzle 44, and the non-inactivated transport liquid can be directly filled and used. It can be used for the detection of pathogens, as well as for the cultivation and separation of pathogens. The non-inactivated transporter of pathogenic microorganisms can inhibit the activity of RNase and DNase, preserve the integrity of RNA and DNA, and realize the storage and transportation of samples at room temperature, among which the inactivation transport The solution can rapidly inactivate pathogens at room temperature, reducing the risk of infection for operators ...

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Abstract

The pathogenic microorganism transfer liquid preparation device comprises an equipment compartment, a liquid mixing mechanism, a filling mechanism and a control terminal, the liquid mixing mechanism is located at one end in the equipment compartment, and the liquid mixing mechanism comprises a first storage box, a second storage box, a third conveying pump and a stirring tank; the filling mechanism is located at the other end in the equipment compartment and comprises a lower conveying belt, an upper conveying belt and a lifting mechanism. The preparation method of the transfer liquid comprises the steps of medicament mixing and medicament filling. The pathogenic microorganism transfer liquid preparation device disclosed by the invention realizes automatic filling of the transfer liquid, is light and convenient to move, meets the detection requirements of sudden and large-scale pathogenic microorganisms in local areas, and is suitable for transferring bacterial samples and RNA virus samples or DNA virus samples after sampling; the non-inactivated transfer liquid can maintain the activity of pathogens to the maximum extent, and can be used for pathogen detection and pathogen culture and separation subsequently.

Description

technical field [0001] The invention relates to the technical field of pathogenic microorganism transport fluid, in particular to a preparation device for pathogenic microorganism transport fluid and a preparation method of the transport fluid. Background technique [0002] In order to comprehensively analyze the causes of viral infection, it is often necessary to collect and extract viruses from patients and analyze and detect to determine the type of viral infection. Live virus can maintain the originality of the sample to the greatest extent, and can be used for virus culture and isolation, as well as for various molecular biological detection of virus. At present, the pathogenic microorganism transport fluids on the market cannot be applied to the transport of bacterial samples and virus samples (virus samples including RNA virus samples and DNA virus samples) after sampling at the same time. The poor survivability of pathogens makes it inconvenient for subsequent detec...

Claims

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Application Information

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IPC IPC(8): B67C3/24B67C3/26B67C3/16B67C3/28B01F35/83B01F35/71B01F33/453
CPCB67C3/24B67C3/2634B67C3/16B67C3/28
Inventor 潘子豪俞菊仙王娟芳
Owner 大能生物科技(南京)有限公司
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