Transportation method based on micro-nano motor and application thereof
A transportation method and micro-nano technology, applied in the field of transportation based on micro-nano motors, can solve the problems of targeted transportation of unfavorable cell cargoes, insufficient biocompatibility, etc., and achieve the effect of small biological damage and strong biological penetration.
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Embodiment 1
[0028] This embodiment provides a light-driven micro-nano motor whose movement mode is rotation. The fabrication method of the light-driven micro-nano motor includes:
[0029] (1) Weigh 0.003 g of up-conversion material (NaYF4:Yb 3+ / Er 3+ Micro-rod particles) powder, added to a centrifuge tube containing 1 mL of ultra-purified water, and then placed in an ultrasonic shaker for shaking to fully mix to obtain an up-conversion material suspension.
[0030] figure 1 The upconversion material (NaYF4:Yb) in this example is shown 3+ / Er 3+ microrod particles). Among them, Figure a is the optical image of the up-conversion material under the scanning electron microscope; Figure b is the microscope bright-field image of the up-conversion material; Figure c is the microscope dark-field image of the up-conversion material; Figure d is the length distribution of the up-conversion material picture.
[0031] (2) Take 20 μL of the up-conversion material suspension with a pipette and ...
Embodiment 2
[0034] In this embodiment, a single photopotential well and multiple photopotential wells are respectively set to rotate the light-driven micro-nanomotor obtained by the method in Embodiment 1, and the specific method is as follows:
[0035] Single optical potential well: place one optical potential well in the center of the assembled cross-shaped structured light-driven micro-nano motor, set the laser power to 90mW, so that the light-driven micro-nano motor is stably captured, and starts to rotate, gradually adjusting the laser Power, the maximum can be adjusted to 1w. The above results show that the light-driven micro-nano motor can be captured and rotated by a single optical potential well, and the rotational speed of the light-driven micro-nano motor can be adjusted by changing the laser power, and the rotational speed adjustment is relatively flexible.
[0036]Multiple optical potential wells: The light-driven micro-nano motor can also be driven to rotate through multiple...
Embodiment 3
[0039] In this example, the light-driven micro-nano motors prepared by the methods in Example 1 are used to arrange and transport particles, and the flow field generated by the light-driven micro-nano motors during rotation is used to transport the particles to the target position non-contact. The specific method as follows:
[0040] Figure 4 Shown is the experimental diagram of arranging light-driven micro-nanomotors into arrays and transporting particles using an optical tweezers system. Among them, Figure a is an experimental diagram of two light-driven micro-nanomotors transporting polystyrene particles; Figures b1-b2 are experimental diagrams of multiple light-driven micro-nanomotors rotating and transporting and changing the direction of movement of polystyrene particles; Figure c1- c3 is the experimental diagram of two light-driven micro-nanomotors targeting the transport of polystyrene particles to cells.
[0041] like Figure 4 As shown in a, a polystyrene solutio...
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