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Construction method and application of lung injury animal model

A lung injury and animal model technology, applied in the fields of pharmaceutical formulation, biological testing, and preparation of test samples, can solve the problems of decreased respiratory compliance of mice, affecting the intensity of lung injury, and not meeting the respiratory rate of mice, etc. , to reduce research costs, avoid stray into the esophagus or nasal cavity, and avoid hidden dangers of infection

Pending Publication Date: 2022-07-29
重庆市急救医疗中心重庆市第四人民医院重庆市急救医学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for the animal model constructed in mice, the invasive method has the following disadvantages: (1) It is invasive, it is difficult to control the risk of infection during the operation, and the mice need to be deeply anesthetized, and the mice die during and after the operation (2) when instilled with granular suspension, it is easy to block the airway and cause the animal to suffocate and die; (3) instilled with solution, it is easy to make the liquid redistribute unevenly in the lungs on both sides
However, this method has the following disadvantages: (1) the device is suitable for rat models, and its volume is too large to be used for mouse modeling; If the administration speed is too fast, the respiratory compliance of the mice is likely to decrease, causing the mice to cough or suffocate, and the mortality rate increases
In addition, some researchers use the nasal drop method to build models (such as Chinese patent CN201910793141.6), but this method is administered through the nasal cavity, and it has been reported in the literature that most of the drugs will stay in the nasal cavity, resulting in a significant reduction in the drug entering the lungs , thus affecting the strength of the drug on lung injury, and the amount of drug entering between samples is also unstable, which will affect the stability of the experiment

Method used

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  • Construction method and application of lung injury animal model
  • Construction method and application of lung injury animal model
  • Construction method and application of lung injury animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Model of acute pulmonary inflammation induced by non-exposed tracheal perfusion CS

[0046] 1. Preparation before the experiment:

[0047] Preparation of CS suspension: Weigh CS granules, add an appropriate amount of normal saline to prepare CS suspension with a final concentration of 60 mg / mL, sterilize by ultrasonic and autoclave before use (need to shake well before administration to make CS in suspension Evenly distributed).

[0048] 2. Tracheal intubation (experimental group)

[0049] 2-1. The mice were weighed before anesthesia, and the mice were anesthetized by intraperitoneal injection of 3% sodium pentobarbital at a dose of 10 mL / kg.

[0050] 2-2. After confirming that the mouse is successfully anesthetized by the tail clamp, use ordinary adhesive tape to fix the mouse limbs on the foam board, and then use rubber bands to fix the mouse upper incisors on the foam board, so that the head and neck of the mouse are in contact with the operator. Keeping...

Embodiment 2

[0070] Example 2 Construction of LPS-induced acute pulmonary inflammation model in mice

[0071] 1. Preparation before the experiment:

[0072] Prepare LPS solution: Add physiological saline to the LPS powder to a final concentration of 0.4 mg / mL. Ready to use after autoclaving.

[0073] 2. The operation methods of each group were referred to in Example 1, except that the administered drug was LPS solution, the mice were anesthetized and sacrificed 24 hours after perfusion, the mortality was recorded, and lung tissue was collected.

[0074] 3. Experimental results

[0075] 3-1. The mortality data of mice are shown in Table 2:

[0076] Table 2

[0077]

[0078] Conclusion: At the end of the experiment, only one mouse died in the present invention, and the mortality rate was only 5%, while the mortality rate of the tracheotomy group was as high as 30%, and the mortality rate of the injection group was also as high as about 20%. are significantly higher than the present i...

Embodiment 3

[0081] Example 3 Construction of CS-induced pulmonary fibrosis model in mice

[0082]1. Preparation before the experiment with reference to Example 1

[0083] 2. The operation method of each group was referred to in Example 1, except that after the mice were returned to the SPF animal breeding room by tracheal perfusion, the mice were anesthetized and sacrificed on the 48th day, the mortality was recorded, and the lung tissue was collected for pathological experiments.

[0084] 3. Experimental results

[0085] 3-1. The comparison data of mouse mortality is shown in Table 3

[0086] table 3

[0087]

[0088] Conclusion: At the end of the experiment, the number of dead mice in the present invention was only 3, and the mortality rate was only 15%, while the mortality rates of the tracheotomy group and the injection group were as high as 45% and 25%, respectively, which was significantly higher than that of the present invention. .

[0089] 3-2. See H&E staining results F...

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Abstract

The invention relates to a construction method and application of a lung injury animal model, and the construction method comprises the following steps: fixing a mouse after anesthesia, exposing the glottis, inserting a trachea cannula suite into the trachea of the mouse, the trachea cannula suite comprises a hollow hose, and a cannula guide wire is arranged in the hollow hose in a penetrating manner; taking out the intubation guide wire in the trachea intubation kit, and placing the hollow hose in the trachea of the mouse; selecting a mouse with uniform breathing, adding the induction liquid into the hollow hose, if the induction liquid in the hollow hose fluctuates up and down along with the breathing of the mouse, taking down the hollow hose after the mouse inhales the required amount of induction liquid, and performing external chest compression to construct the lung injury animal model. The death rate of the obtained animal model in and after the operation is obviously reduced, and the spontaneous respiration is used for replacing the injection and nasal drop mode to ensure that the modeling medicine can better reach the lungs and is uniformly distributed on the lungs on the two sides, so that the adverse effect caused by the difference between groups of the model in the animal experiment is reduced.

Description

technical field [0001] The invention relates to the technical field of animal experimental models, in particular to a method for constructing an animal model of lung injury and its application. Background technique [0002] Lung-injuring diseases include pulmonary injury, chronic obstructive pulmonary disease, pulmonary fibrosis, etc., and are one of the common diseases in clinical practice. Chest pain and other manifestations, and in severe cases, different degrees of respiratory failure and secondary pulmonary infection may occur, endangering the life of patients. [0003] Pathophysiological experiments are mainly carried out on animals, and the type of animal used is an important factor in determining the success of the experiment. Generally for experimental purposes, experimental animal species are selected according to the biological characteristics of experimental animals and the experience of replicating animal disease models. At present, according to the use of exp...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00G01N33/50G01N1/30
CPCA01K67/027A61K49/0008G01N33/5008G01N33/5088G01N1/30A01K2207/30A01K2207/20A01K2227/105A01K2267/03
Inventor 陶莎莎马渝李倩敏杨友静
Owner 重庆市急救医疗中心重庆市第四人民医院重庆市急救医学研究所
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