Novel breast cancer bone metastasis model making mode

Abstract

The invention discloses a novel breast cancer bone metastasis model making method. The method comprises the following steps: 1, taking body position inclination as an operation basis; 2, exposing iliac arteries and veins based on a stereoscopic dissection microscope; 3, arteriovenous separation based on a stereoscopic dissection microscope; 4, preparation before iliac artery injection; 5, injecting tumor cells by using an insulin needle; 6, suturing the wound; and 7, performing postoperative temperature management. Compared with the efficiency and selective modeling of internal iliac artery injection microscopic bone metastasis disclosed by JoVE, the instant success rate of 60% and the survival rate of 20% in 3 days, the breast cancer bone metastasis model making method disclosed by the invention has the advantages that the time for completing iliac artery tumor cell injection to individual BALB / c-nu nude mice at a single time is 15-20 minutes, the instant success rate of breast cancer bone metastasis model making reaches 100%, and the breast cancer bone metastasis model making method has the advantages that the breast cancer bone metastasis model making efficiency is greatly improved. The survival rate in 3 days reaches 80%, the success rate of injecting tumor cells is improved, bleeding of BALB / c-nu nude mice is reduced, and the survival rate of modeling is increased.

Description

technical field [0001] The invention relates to the technical field of bone tumor pain models, in particular to a new modeling method for a breast cancer bone metastasis model. Background technique [0002] Breast cancer metastasis is one of the leading causes of ultimate death, and it can metastasize to almost any part of the human body. Several common sites of metastasis are lymph nodes, bone, liver, lung and brain. Among them, bone is the most common metastatic site of breast cancer, and bone metastases are associated with poor prognosis. Patients with bone metastases develop cancer due to severe pain, reduced mobility, hypercalcemia, osteolysis and fractures, as well as spinal cord compression and bone marrow dysplasia. Late quality of life is extremely poor. [0003] Establishing a mouse breast cancer model is a common method to study breast cancer, which has far-reaching significance for studying the etiology, pathogenesis, finding drug targets, and guiding clinical t...

AI Technical Summary

Problems solved by technology

However, this method of tail vein injection of tumor cells is more likely to form lung and liver metastases, and the probability of successfully establishing a mouse breast cancer bone metastasis model is not high; left atrium injection causes too much trauma to mice, and the survival rate of the model is low. Therefore, tumor cells are usually injected into the iliac artery to establish a mouse model of bone metastasis of breast cancer.

In this regard, the JoVE website published the efficiency and selectivity modeling of internal iliac artery injection microbone metastasis, revealing the iliac artery as the target of injected cancer cells, requiring the iliac vein and artery to be separated from the surrounding tissue, and by using GFP to label Breast cancer cells were labeled with D-fluorescein at the same time to achieve the observation of tumor cell localization in the posterior internal iliac artery. However, in the JoVE iliac artery reference document, it is necessary to use right-angle forceps to insert under the blood vessel for separation, while veins and arteries are in extensive contact with each other, and veins and arteries are in extensive contact with each other. The wall is thin, and it is easy to break due to improper operation. Therefore, it is necessary to design a new and safer molding method to meet the injection needs of saving time and energy.

Images