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Fungus strain for promoting germination of Dendrobium Jinsha River and application thereof

A technology of dendrobium and bacterial strains, applied in the field of plant protection, can solve the problems of low germination rate and hindering the cultivation process of dendrobium seedlings in Jinsha River, etc.

Pending Publication Date: 2022-07-29
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although mycorrhizal fungi of the family Orchidaceae germinate symbiotically with Dendrobium seeds, the germination rate is not high, which greatly hinders the cultivation process of Dendrobium seedlings

Method used

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  • Fungus strain for promoting germination of Dendrobium Jinsha River and application thereof
  • Fungus strain for promoting germination of Dendrobium Jinsha River and application thereof
  • Fungus strain for promoting germination of Dendrobium Jinsha River and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Induction isolation and purification method of Fusarium strain 1-1-2

[0042] 1) Using the ex situ symbiotic germination technology, the bark in the original environment of Dendrobium Jinshajiang was collected back to the laboratory, ground into powder to make a symbiotic germination matrix, and the mature seeds of Dendrobium Jinshajiang were sown. to form seedlings, such as figure 1 shown;

[0043] 2) After picking the protocorm with good growth, cut it in half, put it on the PDA medium, and cultivate it upside down under the constant temperature condition of 28°C in the dark. When the mycelium grows to a certain length, it is purified. The purification process is to continuously pick the mycelium from the edge of the colony to a new PDA medium for serial transfer. After 3 to 5 times of transfer, pure colonies can be obtained. Morphology and molecular identification of the obtained fungi were carried out, and the most suitable germination-promoting strains were scree...

Embodiment 2

[0047] Identification method of Fusarium 1-1-2 strain

[0048] 1) Morphological classification of strains

[0049] Inoculate the strain on PDA medium and observe the morphological characteristics of the colony, such as figure 2 shown: figure 2 A is the characteristic map of the colony morphology of strain 1-1-2 grown on PDA medium for 4 days, the mycelium is milky white, aerial, the colony morphology is regular and round, and the growth rate is 2.000±0.255cm every two days, The culture time was short, covering a 9cm plate in about 7 days. The initial stage was white short-velvet mycelium, and the color of secondary metabolites was pale yellow in the later stage. figure 2 B is a 1-1-2 conidia diagram. There are two types of conidia. The small conidia is oval to cylindrical with 1 to 3 septa; the large conidia is sickle-shaped or long-cylindrical, scattered Born on aerial mycelium, the number of partitions is mostly 3 to 10 partitions, some have more partitions, and the pa...

Embodiment 3

[0055] The Fusarium sp.1-1-2 strain preserved in Example 2 was inoculated on the PDA medium (200g / L potato, 20g / L glucose, 15g / L agar), cultivated for 7d under the constant temperature condition of 28°C, and grew mycelium , to obtain Fusarium sp.1-1-2 strains.

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Abstract

The invention provides a fungus strain for promoting germination of Jinsha River dendrobium and application, and belongs to the technical field of plant protection. The invention relates to a fungus strain for promoting germination of Dendrobium nobile in Jinsha River. The fungus strain is a Fusariumsp. Strain 1-1-2, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M2020553. The strain 1-1-2 is separated from the protocorm of Dendrobium nobile in Jinsha River. After the strain 1-1-2 is co-cultured with the Jinsha River dendrobium nobile, compared with other fungal strains, the strain 1-1-2 can greatly promote the germination of the Jinsha River dendrobium nobile seeds, so that the strain 1-1-2 can be applied to the germination of the Jinsha River dendrobium nobile. Therefore, the method provides favorable conditions for improving the germination rate of the Jinsha River Dendrobium nobile seeds, increasing the population quantity and further developing original habitat protection work.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a fungal strain and application for promoting the germination of Dendrobium officinale in Jinshajiang. Background technique [0002] Dendrobium wangliangii is a typical representative of plants with very small populations in the dry-hot valley of the Jinsha River. It is a window plant for understanding the speciation and evolution of orchids in the dry-hot valley. It has important potential value in both medicinal and ornamental purposes. Due to the combination of narrow distribution area, abnormal climate and human collection, Dendrobium in Jinsha River is currently on the verge of extinction and needs urgent protection. [0003] Increasing the number of individuals by increasing the germination rate of seeds is an important measure to carry out in situ conservation. Mature orchid seeds are extremely tiny and have no endosperm tissue to store nutrients. The...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01C1/00C12R1/77
CPCC12N1/14A01C1/00Y02P60/21
Inventor 赵大克张雪王后平牟宗敏陈穗云
Owner YUNNAN UNIV
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