Detection method of compound Chinese patent medicine containing red ginseng
A detection method and technology for proprietary Chinese medicines, which are applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of unreliable quality control methods, large interference in content detection, and inaccurate quantification, and achieve stable and reproducible content detection methods. Good, high detection efficiency
Pending Publication Date: 2022-07-29
WUHAN KANGLE PHARMA
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Problems solved by technology
[0005] The technical problem solved by the present invention is to overcome the defects in the prior art that the content detection of active ingredients in Shenmai granules has large interference, poor sensitivity, inaccurate quantification, and unreliable quality control means in the production process, thereby providing a red-containing Detection method of ginseng compound Chinese patent medicine
Method used
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Effect test
Embodiment 1
[0054]Preparation of the test solution: take about 50g of Shenmai granules under the item of difference in the loading volume, accurately weigh its mass, add 50mL of water to dissolve it, and then use 50mL and 25mL of water-saturated n-butanol to shake and extract twice, and water bath. The n-butanol was evaporated to dryness, the residue was dissolved in 50% methanol, transferred to a 5 mL volumetric flask, continued to add 50% methanol to the mark, and shaken well.
[0055] Preparation of reference solution: take ginsenoside Rb 1 Appropriate amount of reference substance, accurately weighed, and then add 50% methanol to make a solution with a concentration of 0.1 mg / mL.
[0056] Experimental conditions: octadecylsilane-bonded silica gel column (1.7 μm, 2.1×50 mm), flow rate 0.5 mL / min, column temperature 30° C., detection wavelength 203 nm. The mobile phase ratios are shown in Table 1 below.
[0057] Table 1 Flow phase ratio of Example 1
[0058] time, min Mob...
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Abstract
The invention discloses a method for detecting a compound Chinese patent medicine containing red ginseng. The method comprises the following steps: preparing a test solution; preparing a reference substance solution; setting ultrahigh pressure liquid chromatography conditions: taking acetonitrile as a mobile phase A and water as a mobile phase B, and carrying out gradient elution; the column temperature of the chromatographic column is 28-35 DEG C; the number of theoretical plates is not less than 40000 according to the peak of ginsenoside Rb1; and sucking the test solution and the reference solution, injecting the test solution and the reference solution into an ultrahigh pressure liquid chromatograph, measuring, and recording a chromatogram. The detection method provided by the invention is simple to operate, can effectively reduce the interference of other components on the to-be-detected component, and has high sensitivity. The content detection is stable and accurate, the reproducibility is good, the detection efficiency is high, and the quality of the medicine in the production process of the red ginseng-containing compound Chinese patent medicine (especially the Shenmai granules) can be effectively detected, analyzed and controlled.
Description
technical field [0001] The invention specifically relates to a method for detecting a compound Chinese patent medicine containing red ginseng. Background technique [0002] Shenmai Granules, the main formulations include red ginseng, Nansha ginseng, Ophiopogon japonicus, Huang Jing, Chinese yam, and wolfberry fruit. The main function is to nourish yin and promote body fluid. It is commonly used in clinical practice for sallow skin, thin skin, less thirst, sore waist and knees, loss of appetite, and dizziness. Symptoms of neurasthenia such as vertigo, palpitations, shortness of breath, etc. In order to produce a safe, effective and stable medicine, it is necessary to control its quality during the whole process of production and preparation to ensure that the content of active ingredients is stable and uniform. The current quality control methods for Shenmai granules are character identification and basic physical and chemical identification, such as simple color reaction. ...
Claims
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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李莎卢子龙戴志军武新红范军刘世勇明孟碟郭斐黎俊杰胡倩倩华桂香林诚诚朱家齐饶德林陈日丹程诗旋王磊
Owner WUHAN KANGLE PHARMA