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Extraction process and application of lysimachia christinae polysaccharide

A technology for the extraction of polysaccharides and extracts from orchids, which can be used in preparations for toiletry, antibacterial drugs, anti-toxins, etc., and can solve the problems of the lack of orchids, the combination of active ingredients and pharmacological effects that needs to be further deepened, and the specific effects of active ingredients. Mechanism and pharmacological activity studies are not very clear

Inactive Publication Date: 2022-08-02
普洱学院
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few studies on orchid orchid, and the optimization of polysaccharide extraction process, antioxidant and antibacterial properties of orchid orchid has not been reported in the literature, and the specific mechanism of action and pharmacological activity of active ingredients are not very clear. It is the combination of its active ingredients and pharmacological effects that needs to be further deepened

Method used

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  • Extraction process and application of lysimachia christinae polysaccharide
  • Extraction process and application of lysimachia christinae polysaccharide
  • Extraction process and application of lysimachia christinae polysaccharide

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Embodiment 1

[0029] The present embodiment is an extraction process of Orchid vulgaris polysaccharide, which is characterized in that the optimal extraction process parameters are: time 70min, solid-liquid ratio 1:60 (g / mL), ethanol volume fraction 60%, temperature 50°C, Under this condition, the extraction rate of the polysaccharide from Orchid chinensis was 13.88%;

[0030] Materials: Cinnamon orchid was collected in Jingdong County, Yunnan Province, cleaned and sliced, aired to dryness, crushed, passed through a 60-mesh sieve, sealed at a low temperature and protected from light.

[0031] Reagents: absolute ethanol, sodium hydroxide, ascorbic acid, sodium nitrite, ferrous sulfate, salicylic acid, glucose, petroleum ether, dichloromethane, ethyl acetate, n-butanol (analytical grade): Tianjin Fengchuan Chemical Reagent Technology Co., Ltd.; Aluminum Nitrate (analytical pure): Beijing Compu Huiwei Technology Co., Ltd.; Hydrogen peroxide (analytical pure): Chongqing Chuangdao Chemical Co., ...

Embodiment 2

[0069] The present invention also provides the application of the above-mentioned tube petal orchid in antioxidant and antibacterial;

[0070] Preparation of Orchid Candidum Extract: Preparation of Orchid Candidum extract based on the above-mentioned materials, reagents, instruments and equipment; refer to the method of Wen Bingqin. Add Cymbidium vulgaris powder into the conical flask, and pipette 10 times the volume of 90% ethanol solution, extract 550.01 g of Cymbidium vulgaris powder by ultrasonic at 50°C, extract for 70 minutes, collect the extract after suction filtration, and remove excess by rotary evaporation. ethanol to obtain 19.50 mL of C. japonica extract solution, respectively use petroleum ether, dichloromethane, ethyl acetate, n-butanol (V extractant: V extract solution=1:1) to extract to obtain the extraction of different polar parts liquid, set the temperature at 45°C and put it into an oven to remove excess water to obtain 1.52g of petroleum ether phase extra...

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Abstract

The invention discloses a lysimachia christinae polysaccharide extraction process, which is characterized in that the optimal extraction process parameters are as follows: the time is 70 minutes, the material-liquid ratio is 1: 60 (g / mL), the volume fraction of ethanol is 60%, the temperature is 50 DEG C, and the extraction rate of lysimachia christinae polysaccharide under the conditions reaches 13.88%; based on the characteristics of the lysimachia christinae polysaccharide, the lysimachia christinae polysaccharide is extracted by adopting an ultrasonic-assisted method, and the extraction processes such as the extraction time, the material-liquid ratio, the ethanol volume fraction, the temperature and the like are optimized, so that the method capable of efficiently, quickly, simply and conveniently extracting the lysimachia christinae polysaccharide is obtained. Secondly, comparing the in-vitro antioxidant activity of each extraction phase by measuring the clearance rates of different extraction phases of petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol of the ethanol extract of the turtgrass relative to DPPH. And. OH and the total reducing capacity, and measuring the antibacterial effects of different extraction phases of the turtgrass relative to escherichia coli and staphylococcus aureus by adopting a filter paper method; the application of the lysimachia christinae medicine in physical research and medicinal development directions is explored.

Description

technical field [0001] The invention belongs to the technical field of natural plant active ingredient extraction, and in particular relates to a process for extracting orchid polysaccharide and application thereof. Background technique [0002] Cymbidium is a plant of the Orchidaceae, also known as Bletilla striata. It is distributed in Yunnan, Guizhou, Guangxi, Tibet and other places in my country. It is commonly used in the folk to treat chronic cough and bronchitis, and some of it is used to treat heart disease. It is a folk unorthodox. medicinal orchid. It contains amino acids, carbohydrates, alkaloids, flavonoids and other substances, and has various activities such as antioxidant, anti-tumor, and antibacterial. It has certain research value and market prospects in the fields of medicine, food, and cosmetics. [0003] At present, there are few researches on Cymbidium vulgaris. The optimization of polysaccharide extraction process and the research on antioxidant and ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P31/04A61P39/06A61K8/73A61Q17/00A61Q19/08
CPCC08B37/0003A61P39/06A61P31/04A61K8/73A61Q17/005A61Q19/08Y02A50/30
Inventor 李学玲李梅
Owner 普洱学院
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