Domestication method of synechocystis domestication strain capable of tolerating high light intensity

A Synechocystis, tolerance technology, applied in the field of domestication of Synechocystis domesticated strains, can solve problems such as cell damage and reduced photosynthetic efficiency

Pending Publication Date: 2022-08-05
TIANJIN UNIV
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  • Claims
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Problems solved by technology

In the natural environment, the strongest sunlight can reach 2000 μmol photon m -2 the s -1 , causing serious damage to cells and greatly reducing their photosynthetic efficiency

Method used

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  • Domestication method of synechocystis domestication strain capable of tolerating high light intensity
  • Domestication method of synechocystis domestication strain capable of tolerating high light intensity
  • Domestication method of synechocystis domestication strain capable of tolerating high light intensity

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Embodiment 1

[0025] A method for domestication of a Synechocystis domesticated strain capable of tolerant to high light intensity, comprising the following steps:

[0026] (1) Determine the initial sensitive light intensity of the wild-type Synechocystis strain through an in vitro sensitivity experiment; the specific steps are: inoculate the wild-type bacterial liquid of Synechocystis (Synchocystis PCC 6803 bacterial liquid) in 25 mL of liquid BG11 medium to make the enzyme Determination of OD 750nm is 0.04 at 50-2000 μmol photons m -2 s -1 Under light conditions (respectively: 50, 100, and then take 100 as a gradient, to 2000 μmol photons m -2 s -1 ) Cultivate the wild-type strain of Synechocystis, and determine that the initial sensitive light intensity of the wild-type Synechocystis is 100 μmol photons m -2 s -1 ;

[0027] (2) Inoculate Synechocystis wild-type bacterial solution in 25mL liquid BG11 medium to measure OD with a microplate reader 750nm is 0.04 at 100 μmol photons m ...

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Abstract

The invention discloses a directional domestication method for obtaining a high-light-intensity-tolerant synechocystis strain. The method comprises the following steps: determining the initial sensitive illumination intensity of a wild synechocystis strain; inoculating synechocystis wild-type bacterial liquid into the culture medium to enable OD750nm measured by a microplate reader to be 0.04, and culturing to a plateau phase under initial sensitive illumination intensity; transferring the synechocystis into a culture medium, continuously domesticating, and continuously subculturing until the OD750nm measured by a microplate reader at a plateau phase of the synechocystis is greater than or equal to 0.8; the illumination condition is gradually improved, domestication is continued, continuous subculture is carried out until the illumination intensity reaches 2000 [mu] mol photon m <-2 > s <-1 >, and it is determined that OD750nm of synechocystis is larger than or equal to 0.8 through a microplate reader of the synechocystis in the platform phase. The domesticated strain obtained through the method can grow for 7 days under the highlight condition of 2000 [mu] mol photon m <-2 > s <-1 >, and the content of OD750nm, chlorophyll a, carotenoid, dry weight and glycogen is higher than that of a wild strain.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms, and in particular relates to a domestication method of a Synechocystis domesticated strain capable of tolerant to high light intensity. Background technique [0002] Fossil fuels are non-renewable resources, and excessive use will release large amounts of greenhouse gases into the atmosphere, causing global warming. Therefore, exploring renewable and green bioenergy to replace traditional fossil energy and reduce environmental pollution is an urgent problem we need to solve. Through photosynthesis, about 258 billion tons of CO 2 It is fixed as organic matter, of which terrestrial plants contribute about 30%, and the remaining 70% are contributed by marine photosynthetic microorganisms. Among them, cyanobacteria are the only prokaryotes that can perform photosynthesis and release oxygen, which can contribute about 25% of the total global carbon sequestration. In addition to their tradit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12R1/01
CPCC12N1/20C12N1/36C12R2001/01Y02P60/20
Inventor 陈磊解亚茹孙韬张卫文
Owner TIANJIN UNIV
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