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Preparation method and application of aflatoxin detoxification agent

A technology of aflatoxin and detoxification agent, which is applied in the direction of chemical instruments and methods, oil/fat refining, and other chemical processes. Product safety, good detoxification effect

Pending Publication Date: 2022-08-09
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficiency of removing aflatoxin by biological method is high, but in practical application, the technology needs to be perfected, the conditions are relatively harsh and the cost is relatively high, and large-scale application cannot be realized at present
Most of the modifiers of organically modified montmorillonite are quaternary ammonium salts, which are not food additives. The safety of edible oil adsorption and removal of aflatoxin needs further study

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Weigh 4 g of montmorillonite into a beaker, add 200 mL of ultrapure water, stir for 6 h until fully swollen, and centrifuge at 4500 r / min for 10 min. Collect the precipitate, add it to 200mL of 0.1mol / L hydrochloric acid solution, then slowly add 2CEC histidine modified solution, continue stirring for 24h, centrifuge at 4500r / min for 10min, collect the precipitate, wash and centrifuge repeatedly, and finally wash the washed The precipitate was added with 65 mL of deionized water at a mass-to-volume ratio of 1:15, stirred into a slurry, frozen at -20°C for 12 hours, freeze-dried for 48 hours, and passed through a 200-mesh sieve to obtain aflatoxin remover. A certain 20g peanut oil sample with aflatoxin content of 58.26ppb was placed in a 50mL round-bottomed flask, preheated to 90°C, and aflatoxin detoxifier and stirring bar with a mass ratio of 0.15% relative to peanut oil were added. After vacuuming, stirring for 20min, after adsorption and detoxification, it was transf...

Embodiment 2

[0050] Weigh 4 g of montmorillonite into a beaker, add 200 mL of ultrapure water, stir for 6 h until fully swollen, and centrifuge at 4500 r / min for 10 min. Collect the precipitate, add it to 200mL of 0.01mol / L hydrochloric acid solution, then slowly add 1.5CEC histidine modified solution, continue stirring for 24h, centrifuge at 4500r / min for 10min, collect the precipitate and wash with water and centrifuge repeatedly, and finally wash 65mL of deionized water was added to the precipitate at a mass-to-volume ratio of 1:15, stirred into a slurry, frozen at -20°C for 12h, freeze-dried for 48h and passed through a 200-mesh sieve to obtain aflatoxin remover. A certain 20g peanut oil sample with aflatoxin content of 58.26ppb was placed in a 50mL round-bottomed flask, preheated to 90°C, and aflatoxin detoxifier and stirring bar with a mass ratio of 0.18% relative to peanut oil were added. After vacuuming, stirring for 20min, after adsorption and detoxification, it was transferred in...

Embodiment 3

[0052] Weigh 4 g of montmorillonite into a beaker, add 200 mL of ultrapure water, stir for 6 h until fully swollen, and centrifuge at 4500 r / min for 10 min. Collect the precipitate, add it to 200mL of 0.1mol / L hydrochloric acid solution, then slowly add 2CEC histidine modified solution, continue stirring for 24h, centrifuge at 4500r / min for 10min, collect the precipitate, wash and centrifuge repeatedly, and finally wash the washed The precipitate was added with 65 mL of deionized water at a mass-to-volume ratio of 1:15, stirred into a slurry, frozen at -20°C for 12 hours, freeze-dried for 48 hours, and passed through a 200-mesh sieve to obtain aflatoxin remover. A certain 20g peanut oil sample with aflatoxin content of 200ppb was placed in a 50mL round-bottomed flask, preheated to 90°C, and aflatoxin detoxifier and a stirrer with a mass ratio of 0.5% relative to the peanut oil were added. After vacuum stirring for 20min, after adsorption and detoxification, it was transferred ...

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Abstract

The invention belongs to the field of grain and oil deep processing, and particularly relates to a preparation method and application of an aflatoxin detoxification agent. The preparation method comprises the following steps: obtaining montmorillonite suspension; obtaining an acidic turbid liquid of montmorillonite; adding histidine, mixing, centrifuging, and collecting a second precipitate; washing until chloride ions are not contained, and collecting washing precipitates; and freezing and drying to obtain the aflatoxin detoxification agent. According to the aspergillus flavus detoxification agent, non-toxic and renewable histidine is used as a modifier and a processing aid montmorillonite raw material, montmorillonite is modified in an acid environment, freeze drying treatment is combined to prepare the detoxification agent, the process is simple, the product is safe, and the detoxification effect is good. The aflatoxin removal effect of the detoxification agent is superior to that of the raw material montmorillonite, the aflatoxin removal rate is at least improved by 13% compared with that of the raw material montmorillonite, and the detoxification agent is suitable for removing aflatoxin from most edible oil.

Description

technical field [0001] The invention belongs to the field of fine and deep processing of grain and oil, and more particularly relates to a preparation method and application of an aflatoxin detoxifier. Background technique [0002] Aflatoxins (AFS) are a class of secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus containing a difuran ring and a coumarin (oxaphthalone) structure. Among the chemical carcinogens found so far, aflatoxin is the most toxic and extremely harmful to human health, and has been designated as a first-class carcinogen by the World Health Organization. There are various derivatives of aflatoxin, and aflatoxin B1 is the most toxic and carcinogenic substance, which can cause mutation, teratogenicity and liver damage. The oil crops frequently polluted by Aspergillus flavus include peanuts and corn. Aflatoxin-contaminated peanuts and corn are used as raw materials to prepare edible oil, and aflatoxin is stored in the oi...

Claims

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Application Information

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IPC IPC(8): B01J20/22B01J20/30C11B3/10
CPCB01J20/22B01J20/12C11B3/10Y02W30/74
Inventor 张海龙汪昆立黄俊圻张维农齐玉堂贺军波
Owner WUHAN POLYTECHNIC UNIVERSITY
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