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Tracing marker based on DNA bar code and preparation method thereof

A barcode and marker technology, applied in the field of DNA barcode-based traceability markers and their preparation, can solve problems such as damage to the brand image of geographical indication products, adverse market order stability, infringement of consumer rights and other issues, and avoid chemical erosion and decomposition. , control application cost, improve the effect of nucleic acid stability

Pending Publication Date: 2022-08-09
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Counterfeit goods counterfeit products by false labeling, etc., which damage the brand image of geographical indication products, infringe on the rights and interests of consumers, and are not conducive to the stability of market order.

Method used

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  • Tracing marker based on DNA bar code and preparation method thereof
  • Tracing marker based on DNA bar code and preparation method thereof
  • Tracing marker based on DNA bar code and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1, traceability method based on DNA barcode (traceable marker preparation)

[0080] 1. Experimental method

[0081] 1. Write DNA barcodes carrying traceability information

[0082] Traceability information is compiled according to the rules for writing DNA barcodes for traceability identification. The traceability DNA barcodes include traceability information areas, characteristic identity information areas and amplification-specific primer fragments; The coding length can be adjusted according to actual needs; the traceability information refers to the index table to convert the digitized information into base sequences. The index table used in the design and compilation of DNA barcodes in this embodiment is shown in Table 1 and / or Table 2. On the premise of ensuring the stability of the traceable identification fragments and the amplification effect, the index table 1 in table 1 is preferred.

[0083] The DNA barcode sequence used in this example is shown...

Embodiment 2

[0122] Embodiment 2. Traceability method based on DNA barcode (sequencing identification method)

[0123] In this embodiment, the sequencing identification method is used to detect the traceability markers. Compared with Embodiment 1, the similarities will not be repeated, and only the differences are listed below.

[0124] 1. Sampling of traceability markers

[0125] Obtain the sample on the surface of the traceable object or do pollution-free sampling on the packaging of the traceable object, and separate the traceable markers from the sampling for detection.

[0126] 2. Sequencing read traceability information

[0127] The DNA barcode sequence used in this example is shown in SEQ ID NO. 4. The DNA barcode preparation scheme is to insert traceability information (underlined) and characteristic identity into the mitochondrial COI gene sequence fragment (sequence ID: LC593268.1) of sea cucumber information.

[0128] The experimental process of traceability marker sequencin...

Embodiment 3

[0145] Example 3. Traceability method based on DNA barcode (rapid detection method)

[0146] In this embodiment, a rapid detection method is used to detect traceability markers. Compared with Embodiment 1, the similarities will not be repeated, and only the differences are listed below.

[0147] 1. Experimental method

[0148] 1. Sampling of traceability markers

[0149] Obtain the sample on the surface of the traceable object or do pollution-free sampling on the packaging of the traceable object, and separate the traceable markers from the sampling for detection.

[0150] 2. SRCA amplification detection

[0151] The traceable marker processed with the DNA purification kit was used as the DNA template for the saltatory rolling circle amplification (SRCA) reaction. The DNA barcode sequence used in this example is shown in SEQ ID NO. 9, and the DNA barcode preparation scheme is to insert traceability information (underlined) and features into the mitochondrial COI gene seque...

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Abstract

The invention discloses a source tracing method based on DNA bar codes, which comprises a rapid detection method or a sequencing identification method and comprises the following steps: designing and synthesizing specific DNA and confidential primers according to a source tracing DNA bar code writing principle, and constructing plasmids carrying the DNA bar codes as source tracing marker particles; adding the traceability marker particles in a traceability sample processing link; establishing a database containing the traceability sample, the DNA bar code, the confidential primer, and a corresponding relationship with the traceability source information and / or batch logistics information of the traceability sample for future reference; and the traceability marker is separated from the traceability object, and the authenticity of the traceability information is identified and labeled. The traceability method is high in identification and accuracy, information bearing capacity and identification specificity of DNA are fully utilized, effective identification and accurate traceability of the target object can be achieved, and technical support is provided for maintaining benefits of enterprises and consumers and normal market order.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a DNA barcode-based traceability marker and a preparation method thereof. Background technique [0002] DNA barcoding (DNA Barcoding) technology uses a standard, shorter DNA sequence as a molecular marker to scan one or more genes in an organism to identify a species. As the carrier of genetic information, deoxyribonucleic acid (DNA) has the advantages of high storage density, high parallelism and low energy consumption. The development of synthesis and sequencing technology makes it a possible digital information storage carrier. The manually edited DNA barcode can store the digital information in the base sequence, and realize the compilation and storage of traceability information. [0003] Existing traceability technologies include traditional analytical methods such as high performance liquid chromatography, gas chromatography, and high performance capillary electrophoresis. Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6844C12Q1/6869C12Q1/6806C12Q1/6888C12N15/11C12N15/63G16B30/10G16B30/20
CPCC12Q1/6844C12Q1/6869C12Q1/6806C12Q1/6888C12N15/63G16B30/10G16B30/20C12Q2531/125C12Q2563/185C12Q2563/107C12Q2531/113C12Q2535/122C12Q2527/125Y02P90/30
Inventor 陈颖邢冉冉刘珂涵于宁张九凯邓婷婷
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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