Immunochromatography reagent strip for porcine acute diarrhea syndrome coronavirus antigen and preparation method of immunochromatography reagent strip

A technology for acute diarrhea and coronavirus, which is applied in the field of colloidal gold immunochromatography reagent strips and its preparation, can solve the problems such as the lack of SADS-CoV antigen detection reagents, and achieve the effects of simple operation, easy control, stable storage and convenient carrying.

Pending Publication Date: 2022-08-09
昝洁
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of porcine SADS-CoV is dominated by nucleic acid method, and there is no SADS-CoV antigen detection reagent

Method used

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  • Immunochromatography reagent strip for porcine acute diarrhea syndrome coronavirus antigen and preparation method of immunochromatography reagent strip
  • Immunochromatography reagent strip for porcine acute diarrhea syndrome coronavirus antigen and preparation method of immunochromatography reagent strip
  • Immunochromatography reagent strip for porcine acute diarrhea syndrome coronavirus antigen and preparation method of immunochromatography reagent strip

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Experimental program
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Embodiment 1

[0028] Example 1 Preparation of immunochromatographic test strips of the present invention

[0029] 1. Preparation of colloidal gold solution

[0030] Weigh 100ml of ultrapure water into the conical flask, add 1.0ml of 1% chloroauric acid solution to the conical flask and mix well, when heated to boiling, quickly add an appropriate amount of 1% trisodium citrate, observe the color change of the solution, wait for After the color is stable, continue heating for 5 minutes, then stop heating, cool the solution to room temperature, and store it at 2-8°C.

[0031] 2. Preparation of colloidal gold bonding pads

[0032] (1) Preparation of SADS-CoV-S1 monoclonal antibody:

[0033] Taking the S1 protein of SADS-CoV GDS04 strain (downloaded from the Genbank database) as a reference, common primers were designed, and BamHI and XhoI restriction sites were introduced at the 5' end of the primers. After primer synthesis, PCR amplification was performed using the cDNA of SADS-CoV GDS04 st...

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Abstract

The invention provides a colloidal gold immunochromatography reagent strip for detecting a porcine acute diarrhea syndrome coronavirus antigen. The colloidal gold immunochromatography reagent strip is provided with a carrier plate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane, a porcine acute diarrhea syndrome coronavirus antigen detection line, a control line and a water absorption pad. The colloidal gold immunochromatography reagent strip for detecting the porcine acute diarrhea syndrome coronavirus antigen has the characteristics of rapidness, simplicity, convenience, accuracy, sensitivity and the like in detection, and has relatively high stability; the influence of external factors is small, and the detection sensitivity and accuracy are high; the preparation can be stably stored, is convenient to carry, does not need instruments and equipment, has lower comprehensive cost, and is very suitable for clinical and grassroots units. The test paper is simple to operate and easy to control, and does not need to be operated by professionals.

Description

technical field [0001] The invention belongs to the technical field of in vitro immunodiagnosis, and relates to a colloidal gold immunochromatography reagent strip for detecting porcine acute diarrhea syndrome coronavirus antigen and a preparation method thereof. Background technique [0002] Coronaviruses (CoVs) belong to the subfamily Coronavirinae of the family Coronaviridae, and are enveloped, single-stranded positive-stranded RNA viruses with the largest genome sequence length among known RNA viruses. [0003] At present, swine acute diarrhea syndrome coronavirus (SADS-CoV) has attracted widespread attention in the society, and the detection of viral antigens has always been one of the main methods for the detection of viral diseases. At present, the detection of pig SADS-CoV is dominated by nucleic acid method, and there is no SADS-CoV antigen detection reagent. Therefore, it has become an urgent technical problem to be solved in the art to obtain a kit or test strip ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N33/531
CPCG01N33/56983G01N33/54387G01N33/531G01N2800/065Y02A50/30
Inventor 昝洁陈翠翠梁焕坤赖宏锐郭桂铃李来庆宁波
Owner 昝洁
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