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Biologic sensor

A technology of biosensors and substrates, applied in the field of biosensors, can solve the problems such as the difficulty of sample liquid entering the sample liquid supply channel, the large amount of sample liquid, etc.

Inactive Publication Date: 2004-07-14
PANASONIC HEALTHCARE HLDG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Due to the above characteristics, in the above-mentioned biosensor, there is a tendency that the amount of sample solution required for measurement is larger than that of a biosensor that does not require a carrier.
[0016] In addition, since the carrier is arranged in the sample liquid supply channel, when the sample liquid is blood, if the blood sample is pretreated with a porous body such as filter paper to filter out the blood cell components in the biosensor, sometimes blood cells may be lost. There are some difficulties when the filtered sample solution enters the sample solution supply channel through the porous body for filtration.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0076] In this example, the following method is used to make image 3 The biosensor of the shown structure.

[0077] First, in figure 1 On the electrode system on the substrate 1, 0.5% by weight aqueous solution of the sodium salt of hydrophilic polymer carboxymethyl cellulose (hereinafter referred to as "CMC") was added dropwise, and dried in a warm air drier at 50°C for 10 minutes, A CMC layer 7 is formed.

[0078] Next, 2 μL of a mixed solution of 400 units / mL cholesterol oxidase (hereinafter referred to as “ChOx”) and 2000 units of cholesterol esterase (hereinafter referred to as “ChE”) was added dropwise to cover the CMC layer to form an enzyme layer 18 .

[0079] Then, 1 μL of a 5.0% ethanol solution of TritonX-100 was added dropwise to cover the enzyme layer 18 and dried to form a surfactant layer 19 . The surfactant layer 19 is provided to allow the sample solution to smoothly cover the electrode system, but TritonX-100 was used in order to increase the activity of ...

Embodiment 2

[0088] In this example, the following method is used to make Figure 4 The biosensor of the shown structure.

[0089] Same as Example 1, in figure 1 A CMC layer 7 and a lecithin layer 17 are formed on the electrode system on the substrate 1 . The lecithin layer 17 is also formed on the rear surface of the cover constituting the sample solution supply path.

[0090] Then, potassium ferricyanide, ChOx, ChE, and the surfactant TritonX-100 used to improve the activity of ChE were loaded on a felt glass filter with a thickness of 0.2 mm, a width of 2 mm, and a length of 4.5 mm.

[0091] The loading amount of potassium ferricyanide is 0.33μmol / mm 2 glass filter. The loading of ChOx is 0.1 unit / mm 2 glass filter. The loading amounts of ChE and TritonX-100 were 1 unit and 0.15 mg, respectively.

[0092] A woodworking adhesive (SEMEDAINc manufactured by SEMEDAIN Co., Ltd.) was applied as the adhesive 14 inside the cover plate, and the carrier was bonded and fixed so that the fro...

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Abstract

The present invention provides a biosensor which comprises an insulating base plate; an electrode system including at least a measuring electrode and a counter electrode formed on the base plate; a cover member integrated into the base plate to form a sample solution supply pathway for supplying a sample solution to the electrode system between the cover member and the base plate; a reaction reagent system including at least an electron mediator and an oxidoreductase; and a carrier supporting at least the electron mediator or the oxidoreductase of the reaction reagent system, wherein the carrier is partially or wholly disposed outside the sample solution supply pathway.

Description

Technical field: [0001] The present invention relates to a biosensor capable of quickly and accurately quantifying an object to be measured in a sample in a simple manner. Background technique: [0002] So far, various biosensors have been proposed as means for easily quantifying a specific component in a sample without diluting or stirring the sample solution. One example thereof is the following sensor disclosed in Japanese Patent Laid-Open Publication No. 062952 in 1990. [0003] That is, an electrode system consisting of a measurement electrode, a counter electrode, and a reference electrode is formed on an insulating substrate by screen printing, and then the electrode system is next to the electrode system to form a hydrophilic polymer, redox Enzyme reaction layer of enzymes and electron mediators. A buffer may be added to this enzyme reaction layer as needed. [0004] When the sample solution containing the substrate is dropped on the enzyme reaction layer of the t...

Claims

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Application Information

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IPC IPC(8): G01N27/327C12Q1/00
CPCC12Q1/005G01N27/3272
Inventor 山本智浩渡边基一池田信南海史朗
Owner PANASONIC HEALTHCARE HLDG CO LTD
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