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Pollentube channel transgene improvement technology for corn self line

A technology of pollen tube channeling and inbred lines, applied in the field of plant genetic engineering, can solve the problems of low induction rate of type II embryogenic callus, difficulty in establishing receptor system, and variation of receptor genotype, so as to facilitate popularization and application , short improvement time and low conversion cost

Inactive Publication Date: 2004-12-15
JILIN AGRICULTURAL UNIV
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

The main shortcomings of these two transfer methods are: 1. The induction rate of type II embryogenic callus has obvious differences among genes, and the induction rate of type II embryogenic callus of some genes is extremely low, so it is difficult to establish a receptor system ; 2. It is easy to cause the receptor genotype to mutate during the tissue culture process; 3. The transfer cost is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0007] Transferring Arrowhead Protease Inhibitor Gene (API) into Maize Inbred Line M 0 17. Breed insect-resistant corn inbred lines.

[0008] During the maize flowering period, from the planted M 0 20 robust and typical plants with normal development were selected from the 17 inbred line population for self-bagging.

[0009] The API gene was introduced 8 hours after maize self-fertilization, the paper bag on the corn ear was removed, and the ear filaments near the top of the ear were all cut off with scissors, leaving a flat cut surface, and then a needle micro-sampler was used to introduce the API gene. Drop 200 microliters of the purified API gene DNA solution from the syringe at the incision, put the paper bag back on immediately, and make a record, indicating the time and amount of introduction.

[0010] When the ears of corn are mature, they are harvested, dried in the sun, and threshed to obtain transformed corn kernels.

[0011] According to the selection marker resi...

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PUM

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Abstract

Exogenous destination gene in the present invention is introduced in 6-8 hr after core selfing through shearing out female ear thrumb, dripping 100-300 microliter of exogenous gene DNA liquid in the nick, jacketing with paper bag and recording. The harvested seed after disinfected is made to germinate and grow, the young seedling is polymerase chain reaction detected and molecular crossing analyzed and the converted descendant is selfing purified and agricultural characteristic identified so as to culture transgene corn self line. The present invention is suitable for various type of gene transformation and has high transformation efficiency and short improvement period.

Description

technical field [0001] The invention belongs to plant genetic engineering. Background technique [0002] Maize is one of the most important cultivated crops in the world. It is of great significance to improve maize varieties by using gene technology to transfer foreign target genes into maize. At present, the widely used methods of maize gene transfer are biolistic transformation and Agrobacterium-mediated method using immature embryo callus as the recipient system. The main shortcomings of these two transfer methods are: 1. The induction rate of type II embryogenic callus has obvious differences among genes, and the induction rate of type II embryogenic callus of some genes is extremely low, so it is difficult to establish a receptor system ; 2. It is easy to cause the receptor genotype to mutate during the tissue culture process; 3. The transfer cost is relatively high. Contents of the invention [0003] The purpose of the present invention is to provide a simple, eco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 王丕武张君张晓玲祁新
Owner JILIN AGRICULTURAL UNIV