High yield environmental protection type industrial separating method for ginseng saponin Rb1

Abstract

The present invention relates to the extraction and separation of effective components in Chinese medicine. The ginsenoside Rb1 separating process includes dissolving ginsenoside, American ginsenoside or notoginseng general saponin in small amount of eluant, adding to stabilized chromatographic column to elute while detecting until reaching negative, merging the elutriant of ginsenoside Rb1, reducing recovering solvent, vacuum freeze drying to obtain freeze dried ginsenoside Rb1 powder, inspection and packing. The present invention has the advantages of simple technological process, short production period, no pollution, low cost, high product purity over 95 %, and high yield up to 20 %.

Description

technical field [0001] The invention belongs to a method for extracting and separating active ingredients of traditional Chinese medicines. Background technique [0002] The main bioactive components in ginseng, American ginseng and notoginseng are ginsenosides. The study of ginsenosides began in 1854, when the American scholar Garrigues obtained an amorphous substance from American ginseng produced in Canada, and named it Panaqnilon. 1 , -Rb 2 , -Rc, -Rd and -Re. In 1985, domestic scholar Wei Junxian and others isolated ginsenosides -Ro and -Rb from American ginseng 1 , -Rg 1 , -Re and pseudo-ginsenoside-F11 and other five kinds of ginsenosides. Since the 1990s, on the basis of research at home and abroad, Chinese scholars have further isolated and identified the saponins in ginseng roots and their aerial parts. Analytical methods were systematically studied, and 10 new saponins were obtained from ginseng stems and leaves. So far, more than 40 species of ginsenosides...

AI Technical Summary

Problems solved by technology

[0003] Although in the early 1980s, the industrial production of total ginsenosides from stems and leaves of ginseng had been completed, today, only ginsenoside-Re and ginseng prepared by physical and chemical methods are among the more than 40 kinds of monomeric ginsenosides in total ginsenosides. Saponin-Rg 3 , Enzyme conversion method to produce ginsenoside-Rh 2 Three kinds of monomeric ginsenosides, such as ginsenosides, can be industrialized. Other monomeric saponins are restricted by separation technology, especially the separation process, that is, the previous solvent method and chromatography method. The process is cumbersome, inefficient, difficult to operate, and the production cost is too high. , The solvent is volatile, flammable, and toxic, posing a threat to the personal safety of production personnel and easily polluting the environment. It is limited to the preparation of a small amount of pure products in the laboratory, so it has not been able to realize factory production and industrial development.

And the traditional separation method, ginsenoside Rb 1 The yield is about 10%, and the purity can only reach about 90%. If further purification is required, only by high performance liquid chromatography

The eluent of the traditional separation method needs to be mixed with several solvents. After the production is over, the solvent recovery and separation are difficult and cannot be reused. If separation is required, production equipment needs to be added, resulting in increased production costs.