Compositions to control oral microbial oxidation-reduction (Eh) levels
A composition and compound technology, applied in the direction of drug combination, fluorine compound active ingredient, peroxide active ingredient, etc.
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Embodiment I
[0050] This example demonstrates the ability of saliva supernatant to reduce Eh and glucose as a compound that increases Eh in the saliva precipitation system model developed by Kleinberg [(1967 and 1970) Annals of Oral Biology, 12: 1457-1473; Advances in Oral Biology ( Volume 4), New York, NY, Academy Press, pp. 49-50] in the ability to raise Eh (see figure 2 ). This model uses mixed oral bacteria collected from whole saliva-derived saliva pellets by centrifugation. Extensive studies have shown that this system is metabolically similar to dental plaque (Singer et al. (1983) Annals of Oral Biology, 28:29-35; Wijeyeweera and Kleinberg (1989a and b) Annals of Oral Biology, 34:43-64; Salako and Kleinberg (1992) Annals of Oral Biology, 37:821-829; Ryan and Kleinberg (1995) Annals of Oral Biology, 40:743-752). This model is considered in the art to be a valid paradigm for studying oral microbial metabolism and related biochemical processes. The ability of different amino acids ...
Embodiment II
[0054] A similar type of in vitro experiment as in Example I was performed to analyze the ability of (i) zinc chloride, (ii) hydrogen peroxide and (ii) zinc chloride and hydrogen peroxide to prevent oral Eh decline. Preparations containing 16.7% precipitate, 60 mM phosphate and (i) 6.0 mM (0.08%) zinc chloride, (ii) 0.5% hydrogen peroxide or (iii) 6.0 mM zinc chloride and 0.5% of hydrogen peroxide in the incubation tube. Prepare tubes containing only the saliva pellet and saliva supernatant (water control). figure 2 Indicates the Eh of the mixture during incubation at 37°C for 24 hours.
[0055] like image 3 As shown, zinc chloride and hydrogen peroxide alone or in combination significantly increased the Eh of the incubation mixture compared to the control of saliva supernatant plus saliva precipitate. The control incubation mixture containing only pellet and supernatant showed a rapid and large drop in Eh. The incubation mixtures containing zinc chloride alone and hydro...
Embodiment IV
[0061] This example investigates the ability of a zinc chloride mouthwash to reduce or prevent the formation of volatile sulfur species (VSC) in vivo following a sequential challenge with a cysteine mouthwash. The mouthwash contains 6 mM (0.08%) zinc chloride. The experiments were performed on subjects who had fasted and had not performed oral hygiene for at least 12 hours. Subjects rinsed their mouths with 5 ml of a 6 mM cysteine solution for 30 seconds to stimulate oral bacteria to produce abundant VSCs. This assay is similar to the use of glucose as a challenge substrate to measure plaque bacterial sugar alcoholysis activity (Stephan, R.M., 1994, J. Dental Research, 23, 257) and the intake of a fixed amount of glucose as a challenge substrate to analyze diabetic activity. Before and after mouthwash, the volatile sulfur compounds (VSC) produced were measured with a Halimeter. Simultaneously, Eh was measured by placing a platinum electrode on the dorsal surface of the t...
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