Method for determining red blood cell penetration fragility

Abstract

The invention relates to a determining method for the erythrocyte osmotic brittleness. It adopts turbidimetric analytic method as below: mixing the blood sample with the hypotonic solution with specific osmotic pressure, taking the undissolved erythrocyte as the main particle forming the turbidity of the mixed solution., selecting a undissolved erythrocyte which has higher absorbency to it, taking the specific wave length that the soluble coloured substance such as the free hemoglobin which is dissolved from erythrocyte have a weaker absorbency to it as the determining wave length, making turbidimetric analysis on the spectrophotometer: calculating the value of the haemolytic rate which is in direct ratio with the erythrocyte osmotic brittleness by comparing the different absorbency of erythrocyte in different duration of time and on the same determining condition within the hypotonic solution with the said specific osmotic pressure, or by comparing the different absorbency of erythrocyte between in the isotonic solution and in the above said hypotonic solution with specific osmotic pressure. The invention has the advantages of high accuracy, good repeatability of measurement, simple procedure, high velocity of determination, capability of realizing the dynamic determination and the full automatic analysis easily.

Description

technical field [0001] The invention belongs to a method for clinically measuring osmotic fragility of red blood cells. Background technique [0002] Human red blood cells account for about 40% of the total blood volume, and water and hemoglobin, which contain the most water in red blood cells, account for 65% and 32% of red blood cells respectively. In a hypotonic solution, water penetrates the cell membrane into the red blood cells, causing the red blood cells to gradually swell and rupture. , Intracellular hemoglobin overflow is called hypotonic hemolysis. The resistance of red blood cells to hypotonic fluid hemolysis is called osmotic fragility of red blood cells. In patients with thalassemia and iron deficiency anemia, the production of hemoglobin in red blood cells is reduced, the cell volume is smaller, and the osmotic fragility is significantly smaller than that of normal people. Auxiliary diagnostic indicators. [0003] The existing method for measuring the osmot...

AI Technical Summary

Problems solved by technology

The above method requires manual sample addition, centrifugation, colorimetry, etc., with many determination steps and complicated procedures; the conventional method takes more than two hours, and the improved one-tube method takes more than ten minutes; because the whole process is manual operation, and there are many steps, The reaction conditions are difficult to control, which leads to poor repeatability and accuracy of the results; at the same time, due to the complexity of the reaction system, steps such as centrifugation are required, it is difficult to realize automated analysis

The conventional method needs to judge the results with the naked eye, which is easily affected by human subjective factors, resulting in poor accuracy of the results

In addition, due to the presence of undissolved red blood cells, free hemoglobin and other components in the blood in the reaction solution, the colorimetric analysis method must be centrifuged to accurately measure the results, so dynamic measurement and analysis of the reaction process cannot be realized.