Application of C21 steroid glycoside in pharmacy
A technology of glycosides and steroids, applied in the field of C21 steroid glycosides, can solve the problems of application limitation, toxic and side effects, large side effects, etc., and achieve the effects of low price, small toxic and side effects, and good effects
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Embodiment 1
[0036] Take 10kg of the rhizome of Qingyangshen, crush it, extract it three times with 80% ethanol, combine the extracts, concentrate and recover the solvent. The concentrated solution was diluted with water, extracted three times with petroleum ether, the aqueous layer was extracted three times with chloroform, and the chloroform fractions were combined. Chloroform was recovered to obtain 300 g of light yellow powder, with a yield of 3.0%. The obtained light yellow powder is total glucosides of Qingyangshen. Taking Qingyangshen aglycone as a reference substance, the content of total glycosides is 72.5% according to the method of pharmaceutical standard (WS3-B-2343-97). The content of main aglycones in total glycosides was determined by HPLC method, and the results were: Qingyangshen aglycone content was 5.1%, and Kodatin content was 10.5%.
Embodiment 2
[0038] Take 10 kg of rhizomes of Kunming vine crown, crush them, extract three times with 80% ethanol, combine the extracts, concentrate and recover the solvent. The concentrated solution was diluted with water, extracted three times with petroleum ether, the aqueous layer was extracted three times with chloroform, and the chloroform fractions were combined. Chloroform was recovered to obtain 310 g of light yellow powder, with a yield of 3.1%. The obtained light yellow powder is the total glucosides of Kunming cuprosides. Taking qingyangshen aglycone as the reference substance, referring to the method of the pharmaceutical standard (WS3-B-2343-97), the measured total glycoside content is 80.4%. The content of main aglycones in the total glycosides was determined by HPLC, and the results were: the content of Qingyangshen aglycone in the total glycosides was 6.0%, and the content of kudatingin was 15.2%.
Embodiment 3
[0040]Take 50g of total glycosides of Qingyangshen, dissolve it in 200ml of methanol, add 200g of silica gel with a particle size of 100 mesh, stir evenly, and evaporate the methanol to dryness. Take 1000 g of silica gel with a particle size of 300-400 mesh, and perform column chromatography according to a conventional method, and the elution system is chloroform-methanol (20:1-5:1), gradient elution. The eluate was collected to obtain 5 fractions, and the third fraction was subjected to repeated column chromatography on reversed-phase silica gel, eluting with methanol: water (60:40), to obtain 11.2 g of compound, which was qingyang shenside A. White powder, molecular formula: C 56 h 86 o 16 , Molecular weight: 932. Soluble in ethanol, methanol, ethyl acetate, chloroform.
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