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Human blood high density lipoprotein and its preparation method and use

A high-density lipoprotein and manufacturing method technology, applied in the field of biochemistry, can solve problems such as preparing HDL, and achieve the effects of inhibiting the occurrence of AS, promoting the reversal or regression of AS, and reducing the plaque area

Inactive Publication Date: 2007-06-13
江永忠 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is currently no method for directly producing HDL from plasma

Method used

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  • Human blood high density lipoprotein and its preparation method and use
  • Human blood high density lipoprotein and its preparation method and use
  • Human blood high density lipoprotein and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Take 1000L of raw plasma, add dropwise 25L of HAC / NaAC buffer solution with pH=4.0, adjust the pH of the mixture to=5.2, add 324L of 95% ethanol, stir at -5℃ for 2 hours, stand for 6 hours, use 142 type continuous Centrifuge at 14000r / min to get 1340L of supernatant and 50Kg of precipitation;

[0064] Add 630L of water for injection to the supernatant, drop 13.7L of the HAC / NaAC mixed solution with pH 4.0 to make the pH of the mixture 4.5, at this time the protein concentration is 1%, the ionic strength is 0.05, and stir for 2 hours at -3°C , Stand for 2 hours, use 142 continuous centrifuge, centrifuge at 14000r / min, get 9Kg of precipitate, discard the supernatant;

[0065] Add 19.8L of 30mmol / L sodium chloride solution to the precipitate, drop 14L of HAC / NaAC mixed solution with pH 4.0 to pH 5.5, stir at 0℃ for 4 hours, use 142 type continuous centrifuge, at 14000r / Centrifuge at min, discard the supernatant, get 4.65Kg of precipitate;

[0066] Add 160mmol / L sodium chlorid...

Embodiment 2

[0071] Take 1000L of raw plasma, add dropwise 24.5L of HAC / NaAC buffer solution with pH=4.0, adjust the pH of the mixed solution to 5.15, add 330L of 95% ethanol, stir for 2 hours at -4°C, stand for 8 hours, use type 142 Continuous centrifuge, centrifuge at 14000r / min, get 1270L of supernatant and 47Kg of precipitation;

[0072] Add 646L of water for injection to the supernatant, drop 14.3L of the HAC / NaAC mixed solution with pH 4.0 to make the pH of the mixture 5.2, the protein concentration is 1.68%, and the ionic strength is 0.07. Stir at -6°C for 2 hours , Let stand for 2 hours, use 142 continuous centrifuge, centrifuge at 14000r / min, get 9.5Kg of precipitate, discard the supernatant;

[0073] Add 19.0L of 30mmol / L sodium chloride solution to the precipitate, drop 13.7L of the HAC / NaAC mixed solution with pH 4.0 to make the pH 5.7, stir at 1℃ for 4 hours, use 142 type continuous centrifuge at 14000r Centrifuge at / min, discard the supernatant, and get 4.33Kg of precipitate;

...

Embodiment 3

[0079] Take 1000L of raw plasma, add dropwise 24.3L of HAC / NaAC buffer solution with pH=4.0, adjust the pH of the mixture to=5.22, add 304L of 95% ethanol, stir at -5°C for 2 hours, stand for 6 hours, use type 142 Continuous centrifuge, centrifuge at 14000r / min, get 1299L of supernatant and 45Kg of precipitation;

[0080] Supplement the supernatant with 611L of water for injection, dropwise add 14L of the HAC / NaAC mixed solution with pH 4.0 to make the pH of the mixture 5.1, the protein concentration is 1.68%, and the ionic strength is 0.07. Stir at -6°C for 2 hours. Let stand for 2 hours, use 142 continuous centrifuge, centrifuge at 14000r / min, get 8.9Kg of precipitate, discard the supernatant;

[0081] Add 18.9L of 30mmol / L sodium chloride solution to the precipitate, add 13.6L of HAC / NaAC mixed solution with pH 4.0 dropwise to make the pH 4.9, stir at 0℃ for 4 hours, use 142 type continuous centrifuge, at 14000r Centrifuge at / min, discard the supernatant, and get 4.05Kg of pre...

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Abstract

The invention relates to a human high density lipoprotein and product, preparation process and use thereof, wherein the apo AI content in the human high density lipoprotein is 77-89%. The preparation process comprises using blood plasma as raw material, extracting HDL through segregation and purification steps. The prepared human high density lipoprotein and products produced thereby can be applied to the preparation for medicaments for treating multiple organ dysfunction syndrome (MODS), cardiovascular system diseases such as atherosclerosis (AS).

Description

Technical field [0001] The invention relates to a human blood high-density lipoprotein, its manufacturing method and its application in pharmaceutics, and belongs to the field of biochemistry. Background technique [0002] AS is a common multiple disease in which the arterial wall is thickened and hardened due to lipid deposits in the arterial wall, and the hyperplasia of fibers and connective tissues, and the elasticity decreases and the formation of chylo-atheromas. The occlusion of the arterial lumen caused by AS can make the heart, brain, kidney and other important organs insufficient blood supply, thereby seriously threatening human health and life. At present, the commonly used drugs for the treatment of AS in clinical practice generally include vasodilator drugs, hypolipidemic drugs, antiplatelet drugs and thrombolytic drugs. Although these drugs can fight against AS by removing vascular dyskinesia and regulating blood lipids, they cannot prevent and eliminate AS plaques s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K14/47C07K1/34A61P9/10A61P3/06A61P31/04A61K38/17
Inventor 江永忠陈爱民周潮樊绍文
Owner 江永忠
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