Gene chip colorating detection method

Abstract

In the method, digoxin or biotin mark is added in the PCR augmentatino course of the sample to be tested and then the PCR product is hybridized with the chip, by adding colour developing liquid to obtain the correspounding signal after hybridizing and combining the antibody with digoxin or biotin mark. The correspounding results will be collected and detected with normal optical scanners scanning.

Description

technical field [0001] The invention relates to a gene chip, in particular to a color detection method of the gene chip. Background technique [0002] DNA chip (gene chip) is one of the major scientific and technological developments with the most epochal characteristics in the high-tech field in recent years. It is a high-tech cross-combination of microelectronics, physics, chemistry, material science and life science. DNA chips use the intensification and parallel processing principles of microelectronic chip technology to orderly solidify a large number of biological samples with characteristic sequences of biological significance on silicon substrates or glass, and use DNA chips to quickly acquire or process a large number of Life information (including gene identification, gene mutation detection, gene expression profile detection, etc.), which has been successfully applied in the fields of gene expression, gene mutation, gene polymorphism research and gene diagnosis. ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a color detection technology that does not require expensive detection instruments for the problems existing in the current gene chip detection. On the one hand, this technology can use a cheap ordinary optical scanner to scan, and The obtained map is input into the computer for image analysis and data arrangement. On the other hand, it can also be directly observed with a magnifying glass or the naked eye when there is less information; at the same time, during the sample amplification process, digoxin or biotin is labeled on In terms of primers, the cost of using the chip is greatly reduced, which can completely improve the current situation that the chip is expensive and the chip detection equipment is expensive and difficult to promote.

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