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Multi-enzyme product comprising glucoumylolytic, proteolytic and xylanolytic activities and process for preparing the same

A technology of xylanase activity and glucoamylase, applied in the field of multi-enzyme products, can solve the problems of increasing wheat ethanol cost, expensive, complicated methods, etc.

Inactive Publication Date: 2002-02-27
GIE AGRO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] All these enzymes are individually available in pure form on the market, but the disadvantage is that they are relatively expensive, thus increasing the cost of producing ethanol from wheat
In addition, the composition should be formulated from a single enzyme, which complicates the process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The selection of embodiment 1-Aspergillus strain

[0099] The ability of seven commercially available Aspergillus strains to produce glucoamylase by fermentation in a solid medium of wheat bran was studied in a comparative manner.

[0100] Tests were carried out with 50 g of fermentation medium in Erlenmeyer flasks. This medium contains 21.5 grams of wheat bran, 27.5 grams of water and 1 gram of wheat starch. The initial pH of this medium is 6.0-6.5. The medium is sterilized in an autoclave at 120°C for 20 minutes.

[0101] Each medium was inoculated with 2 × 10 per gram of initial dry matter 7 spores of the strains to be tested. The spore age is 3 days. After fermenting for 40-50 hours, put the Erlenmeyer flask into an oven at 35°C. At the end of the fermentation, the fermentation medium was mixed with 150 ml of distilled water to make a solution of the obtained enzyme, and then the enzyme solution was recovered by filtration. The solution was centrifuged to rem...

Embodiment 2

[0105] Example 2 - Preparation of glucoamylase with 50 liters non-sterile pilot tank supplied by INRA: Importance of wheat bran pretreatment

[0106] The test was carried out according to the method described in the article by A. Durand et al. (Fig. 1) using non-sterile 50 liter fermenters and BCE wheat bran (supplied by Brie champagne ethanol factory in Provins, France). Two modes of wheat bran preparation were implemented to obtain 5 kg of culture medium with a moisture content of 55%:

[0107] - dry wheat bran: wheat bran is treated in an autoclave at 105 °C for 1 hour and then mixed with water (test F4C3);

[0108] - Wet bran: The bran was humidified to 45% in a mixing tank and autoclaved at 121° C. for 20 minutes (test F4C4).

[0109] In both cases, use 2×10 7 spores per gram of dry matter and adjust the water content of the medium to about 55%. Fermentation then takes place in ventilated tanks on layers 10 cm high. During this incubation period, the temperature was l...

Embodiment 3

[0116] Example 3: Production of glucoamylase with a pilot fermenter supplied by Fujiwara: Importance of maintaining medium humidity during fermentation

[0117] The experiment was carried out with a pilot fermenter and BCE wheat bran sold by Fujiwara Co., Ltd., Okayama, Japan. The difference from the fermenter used in Example 2 is in particular that this tank has a diameter of 0.66 m whereas the diameter of the INRA tank is 0.35 m. In this fermentor, 20 kg of a medium containing 55% water, prepared according to the wet bran mode described in Example 2, was required for cultivation at a thickness of 12 cm. Agitation is ensured by three vertical worms rotating at constant speed, which will mix the media in the rotating (5-10 rpm) tank. During the fermentation, as in the INRA tank of Example 2, the gas atmosphere was constantly renewed by conditioned air whose temperature, humidity and flow rate are shown in Table 4.

[0118] During this trial, called FII, the timing of adjusti...

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PUM

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Abstract

Enzyme preparation (I) produced by fermenting wheat bran with an Aspergillus niger strain has glucoamylase, protease and xylanase activities of at least 100 U / g (dry basis), provided that the glucoamylase activity is at least 750 U / g and / or the xylanase activity is at least 300 U / g. An Independent claim is also included for the production of (I) by: (1) moistening and pasteurizing or sterilizing wheat bran; (2) inoculating an at least 10 cm thick layer of the bran with Aspergillus niger; and (3) fermenting the bran in an aerated and periodically agitated reactor at 28 - 38 degrees C for 1 - 3 days while maintaining the moisture content of the bran at 50 - 60 wt.%.

Description

technical field [0001] The present invention relates to a multi-enzyme product having glucoamylase, proteolytic ester and xylanase activities and a method for preparing the product by solid-state fermentation of wheat bran with the aid of Aspergillus niger. Background technique [0002] It is known to produce ethanol from cornstarch by means of an enzymatic process comprising the steps of liquefying the starch with alpha-amylase to hydrolyze the starch to dextrins, followed by glucoamylase (also known as amyloglucosidase) Saccharification is performed to hydrolyze dextrins to glucose and finally to ferment glucose to ethanol. [0003] The use of alpha-amylases and glucoamylases is generally satisfactory when starting from relatively pure starch emulsions obtained by wet grinding corn, but is used when it is desired to substitute wheat starch or flour for corn starch. Satisfactory results were not obtained with the two enzymes alone because the presence of hemicellulose incr...

Claims

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Application Information

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IPC IPC(8): A23K1/165C12N1/14C12N9/24C12N9/30C12N9/34C12N9/58C12N9/62C12P7/06C12R1/085
CPCY02E50/17C12N9/2482C12Y302/01003C12N9/62C12N9/24C12N9/2428C12N1/14C12Y302/01008Y02E50/10
Inventor P·J·拉贝勒J-L·A·G·贝尔特F·L·杜奇昂
Owner GIE AGRO IND
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