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Method for examination of feces ocoult blood

An inspection method and technology for fecal occult blood, which are applied in the field of fecal occult blood inspection, can solve the problems of fluctuation of occult blood inspection value, large difference in stool quality, and lack of high-precision inspection value, and achieve the effect of good accuracy

Inactive Publication Date: 2003-03-05
INTERNATL REAGENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now people have adopted various methods to the feces collecting containers sold on the market, trying their best to make them collect a certain amount of feces, but the quality of feces varies greatly between individuals and every day, and its moisture content varies, so it may not be possible to carry out accurate monitoring. take stool
As a result, there is a problem that the test value of fecal occult blood fluctuates, and a highly accurate test value cannot be obtained.

Method used

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  • Method for examination of feces ocoult blood
  • Method for examination of feces ocoult blood
  • Method for examination of feces ocoult blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 40 cases (28 cases of males, 12 cases of females) were fully stirred and mixed with fresh stools, and 1-4 g of them were accurately collected into containers as samples of the experimental examples shown below.

[0047] (Experimental Example 1-1) After freezing 40 samples at -80° C. for 2 hours, they were dried under reduced pressure in a desiccator using a vacuum pump, and the weight after drying was accurately weighed. The moisture content of each feces was obtained by dividing the weight difference before and after drying by the weight before drying. The results are shown in Table 1.

[0048] Moisture content (%)

50-55

56-60

61-65

66-70

71-75

76-80

81-85

86-90

total

ratio

(%)

year

age

layer

0-10

2

2

5

11-20

1

1

2

5

21-30

1

1 ...

Embodiment 2

[0055] Fecal occult blood was positive in 3 cases, and the feces of each case were collected 10 times in a commercially available stool collection container (manufactured by International Pharmaceutical Co., Ltd.). The hemoglobin concentration of each sample was measured by latex turbidimetry using a fecal occult blood assay kit (Shima Research Institute) according to its instruction manual.

[0056] The results were 243-512, 52-154, and 857-1997 ng / ml, respectively. On the other hand, based on the absorbance at a wavelength of 450nm, applying the relational formula obtained in Experimental Example 1-3, and correcting the hemoglobin concentration, the values ​​are 134-155, 59-90, 314-641ng / ml respectively, which reduces the Errors caused by defecation. The result is as figure 2 shown. (Example 3)

Embodiment 3

[0057] (Experimental Example 3-1) The feces of patients with fecal occult blood positive were uniformly mixed, and feces were collected from 10 locations using a commercially available stool collection container designed to dilute 10 mg of stool with 2 mL of buffer solution. The concentration of hemoglobin (Hb) in the collected stool samples was determined by using latex reagents that can absorb two kinds of anti-human hemoglobin antibodies (mAbs). At the same time, CEA was determined using a commercially available CEA determination EIA kit (International Laboratory Co., Ltd.).

[0058] Based on the measured values ​​obtained, the average value, maximum value-minimum value, and coefficient of variation of the Hb concentration were calculated. Calculate the ratio of each measured value of Hb to the CEA value, and calculate the average value, maximum value-minimum value and coefficient of variation respectively. The results are shown in Table 2. In addition, the amount of Hb (n...

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Abstract

There is provided a method where a feces occult blood test with a good precision is possible regardless of the sampling precision of feces. The above-mentioned method is achieved when the feces amount is corrected by an index being able to reduce the amount of the sampled feces to be tested in the measurement of hemoglobin and / or transferrin which are / is marker (s) for feces occult blood. Thus, together with the measurement of the feces occult blood marker, a signal obtained by the optical measurement of a suspension of the feces to be tested and / or the amount of the indigenous substances in the feces to be tested being able to be the above index and then the measured value of the feces occult blood marker is reduced using the measured value of the said index as a control whereby the above object is achieved.

Description

technical field [0001] The invention relates to a method for examining fecal occult blood. More specifically, the method for examining fecal occult blood according to the present invention is characterized in that, when measuring the fecal occult blood markers, changes in the fecal occult blood markers due to individual differences in the amount of feces collected are corrected. In addition, the present invention relates to means and devices used in clinical examination of fecal occult blood. Background technique [0002] The examination methods for fecal occult blood caused by gastrointestinal bleeding include chemical examination and immunological examination. The chemical detection method is a method of judging the peroxidase-like activity of hemoglobin by oxidizing and developing color of chromogen. In this case, the feces are applied to the filter paper permeated with the chromogen, and the color developing solution hydrogen peroxide is added dropwise to the applied f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72
CPCG01N33/721G01N2333/79G01N33/728
Inventor 上村八寻日裹久英永松刚落合浩二
Owner INTERNATL REAGENTS CORP
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