Microorganism producing L-lysine and processes for producing L-lysine usi ng the same

A technology of lysine and Corynebacterium glutamicum, which is applied in the field of L-lysine-producing microorganisms and its production of L-lysine, and can solve the problems of no reported resistance to the antibiotic monensin

Inactive Publication Date: 2003-03-26
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no strains resistant to the antibiotic monensin have been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Corynebacterium glutamicum CH77 and CJM107 were inoculated in a 250 ml elbow baffled bottle containing 25 ml of the following seed medium, and cultured at 30° C. for 20 hours with shaking (220 rpm). Inoculate 1 ml of seed medium into a 250 ml elbowed bottle with baffles containing less than 25 ml of production medium, and culture at 32° C. with shaking (220 rpm) for 96 hours. After the cultivation was completed, the amount of L-lysine was measured by HPLC. The L-lysine present as L-lysine hydrochloride in the culture solutions of Corynebacterium glutamicum CH77 and CJM107 were 47 g / l and 51 g / l, respectively. Seed medium (pH7.0)

[0020] Raw sugar 50g, peptone 10g, yeast extract 10g, urea 5g, KH 2 PO 4 4g, K 2 HPO 4 8g, MgSO 4 ·7H 2 O0.5g, biotin 100 μ g and thiamine hydrochloride 1000 μ g (per 1 liter of industrial water) production medium (pH7.0)

[0021] Molasses or pre-treated molasses (with reducing sugar) 50g, raw sugar 50g, yeast extract 4g, (NH 4 ) 2 S...

Embodiment 2

[0023] The CJM107 strain was grown in a medium containing molasses and raw sugar. After culturing, sulfuric acid or hydrochloric acid was added to 1 liter of L-lysine fermentation broth to adjust the pH to 2.0, thereby reducing the Ca 2+ converted to CaSO 4 or CaCl 2 . Next, the broth was applied to allow countercurrent adsorption to cation exchange resins (Dianion SK-1B, SK-1BL), which were regenerated in the form of ammonium ions. Subsequently, it was washed with desalted water to remove cell aggregates remaining in the resin layer, and then a concentrated fraction of L-lysine was recovered therefrom by elution with 2N ammonium hydroxide. After concentrating the recovered fraction, the concentrate was adjusted to pH 5.0 and cooled to 20° C., thereby forming L-lysine crystals.

[0024] The slurry obtained after complete crystallization was centrifuged to obtain a first wet product. The mother liquor was again concentrated and crystallized by batch method to obtain a seco...

Embodiment 3

[0025] The CJM107 strain was grown in a medium containing pretreated molasses and raw sugar. After cultivation, sulfuric acid was added to 1 liter of L-lysine fermentation broth to adjust the pH to 2.0, thereby reducing the Ca 2+ converted to CaSO 4 . Next, the broth was applied to allow countercurrent adsorption to cation exchange resins (Dianion SK-1B, SK-1BL), which were regenerated in the form of ammonium ions. Subsequently, it was washed with desalted water to remove cell aggregates remaining in the resin layer, and then a concentrated fraction of L-lysine was recovered therefrom by elution with 2 N ammonium hydroxide. After concentrating the recovered fraction, the concentrate was adjusted to pH 5.0 and cooled to 20° C., thereby forming L-lysine crystals.

[0026] The slurry obtained after complete crystallization was centrifuged to obtain a first wet product. The mother liquor was again concentrated and crystallized by batch method to obtain a second wet product. T...

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PUM

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Abstract

Disclosed are a Coryneform bacterium having resistance to an antibiotic, monensin, and producing L-lysine, and a process for producing L-lysine by a direct fermentation, which comprises culturing said microorganism in a fermentation medium, accumulating L-lysine in the resulting culture broth, and recovering L-lysine therefrom.

Description

technical field [0001] The present invention relates to an L-lysine-producing microorganism and a method for producing L-lysine using the same. More specifically, the present invention relates to Corynebacterium glutamicum CJM107 (KCCM-10227), which is resistant to the antibiotic monensin and capable of producing L-lysine at a high yield, and relates to A method for producing L-lysine by fermenting the microorganism. Background technique [0002] Lysine, one of the basic amino acids, is widely used as animal feed, medicine, food, etc. The demand for lysine has recently increased due to the tendency to prevent environmental pollution from livestock wastewater by strengthening the regulation of nitrogen- or phosphorus-containing components in livestock excrement. It is estimated that in 1999, the market size of L-lysine as an animal feed additive was about 500,000 tons, and the demand for it is expected to increase continuously at an average annual rate of about 10%. As men...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/21C12P13/08C12R1/15
CPCC12P13/08C12R1/15C12R2001/15C12N1/205C12N1/20
Inventor 金成俊李庚翰成珍锡林相曹张在佑
Owner CJ CHEILJEDANG CORP
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