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Preparation of implanting neurons for treating nerve injury diseases

A neuron and disease technology, applied in nervous system diseases, biochemical equipment and methods, nervous system cells, etc., can solve the problems of decreased immune function, dysfunction, danger, etc., to achieve good growth, easy access, and no immune rejection. Effect

Inactive Publication Date: 2003-06-18
SUN YAT SEN UNIV
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AI Technical Summary

Problems solved by technology

Once the patient's body condition changes in the future (such as infection or immunosuppressive drugs leading to reduced immune function), the implanted cells may differentiate into other tissue cells, causing dysfunction and the risk of disease

Method used

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Examples

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Embodiment Construction

[0010] The invention separates, cultures, expands and optimizes human or rat bone marrow mesenchymal stem cells, and induces bone marrow mesenchymal stem cells to differentiate into neurons with musk polypeptide or Danshen injection respectively.

[0011] 1. Isolation, culture, expansion and identification of biological characteristics of bone marrow mesenchymal stem cells.

[0012] Take 3-5 ml of human or rat bone marrow, and separate them in the separation medium with a specific gravity of 1.078. When cultured in low-glucose medium (L-DMEM) containing 15% fetal calf serum (FCS), the mesenchymal stem cells quickly adhered to the wall, and other cells did not adhere to the wall. After 2-3 passages, the purpose of purification is basically achieved. The expression of CD116, CD45, CD61, CD71, CD80, CD86, and MHC-II on the surface markers of the fifth generation cells were all negative, while CD29 and CD44 were positive, proving that they were mesenchymal stem cells rather than ...

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Abstract

A process for preparing neurocyte implant used to cure the nerve injury diseases includes such steps as separating bone marrow stem cells from human or amimal's bone marrow, culturing, amplifying, inducing the differentiation of bone marrow stem cells by red sage root, injection and musk polypeptide to become neurocytes, and washing. Its advantages are high safety, high curative effect and no immunological rejection.

Description

technical field [0001] The invention relates to a method for inducing mesenchymal stem cells to differentiate into neurons, in particular to a method for preparing implanted neurons for treating nerve injury diseases by using traditional Chinese medicine as an inducer. Background technique [0002] Nerve injury diseases such as paralysis caused by spinal cord injury and Parkinson's disease are common clinical diseases, but there is no effective treatment at present, which has brought huge economic burden and heavy burden to patients, their families and society. Mental burden. Bone marrow mesenchymal stem cells are implanted into the body and differentiated into neurons, which can replace damaged nerve cells and rebuild the function of damaged nerves, so as to achieve the purpose of treatment. However, bone marrow mesenchymal stem cells are cells with multiple differentiation potentials. If they are directly implanted into the body, some quiescent cells still maintain the ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61L27/00A61P25/00A61P25/16C12N5/0793
Inventor 李树浓邓宇斌肖庆忠
Owner SUN YAT SEN UNIV
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