Process of preparing polymer porous leg by separation and combination of paraffin microballons porous making agent and thermotropic phase

A thermally induced phase separation method and thermally induced phase separation technology, which are applied in medical science, prosthesis, surgery, etc., can solve the problems of difficult to obtain large-sized pores, difficult to precisely control the pore size, lengthy preparation process, etc., and achieve porosity High, easy to operate, good hole connectivity effect

Inactive Publication Date: 2003-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the porogen method alone, in order to obtain scaffolds with good pore connectivity, there are disadvantages of lengthy preparation process and low porosity.
For the thermally induced phase separation method, the advantage is that the obtained porous scaffold has good pore connectivity, but it has the disadvantage that the pore size is not easy to control precisely, especially it is difficult to obtain large pore size

Method used

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  • Process of preparing polymer porous leg by separation and combination of paraffin microballons porous making agent and thermotropic phase
  • Process of preparing polymer porous leg by separation and combination of paraffin microballons porous making agent and thermotropic phase
  • Process of preparing polymer porous leg by separation and combination of paraffin microballons porous making agent and thermotropic phase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation of poly-L-lactic acid (PLLA) porous scaffold

[0029] Wax ball particles were prepared by suspension method. In an Erlenmeyer flask, add 40g of solid paraffin and 200mL of 20mg / mL aqueous gelatin solution, heat to 80°C, and the paraffin becomes liquid. Stir with an electromagnetic stirrer at a stirring speed of 200 rpm to disperse the liquid paraffin into small droplets. After the state of the paraffin suspension is stable, it is quickly poured into ice water at 0° C. for quenching to obtain solid paraffin microspheres. The paraffin microspheres were classified by domestic standard sieves, and the paraffin microspheres with a particle size of 150-450 μm were obtained.

[0030] In a stainless steel mold, the paraffin microspheres were uniformly mixed with the PLLA / dioxane solution with a concentration of 0.06 g / mL, and the amount of the paraffin microspheres was 1.3 g per milliliter of the PLLA solution. The above mixture was frozen at -2...

Embodiment 2

[0031] Embodiment 2: the control of poly-L-lactic acid (PLLA) porous scaffold aperture

[0032] Prepare paraffin microspheres according to the method in Application Example 1, and use domestic standard sieves to divide paraffin microspheres into different fractions below 150 μm, 150-280 μm and 280-450 μm according to the particle size, such as figure 2 a. figure 2 b and figure 2 as shown in c.

[0033] The above-mentioned paraffin microspheres with different particle sizes were used to prepare PLLA porous scaffolds according to the method in Example 1, wherein the concentration of PLLA solution was 0.05 g / mL, and the amount of paraffin microspheres was 1.6 g per milliliter of PLLA solution. The laser confocal micrographs of the obtained PLLA porous scaffold are as follows: image 3 a. image 3 b and image 3 as shown in c. Depend on image 3 It can be seen that the pore size of the porous scaffold can be conveniently controlled by controlling the particle size of the p...

Embodiment 3

[0034] Embodiment 3: the control of the porosity and apparent density of poly-L-lactic acid (PLLA) porous scaffold

[0035] Paraffin microspheres with different particle sizes, different concentrations of PLLA solutions, and different dosages of paraffin microspheres were used, and the PLLA porous scaffold was prepared according to the method in Example 1. Table 1 summarizes the porosity and apparent density of the PLLA porous scaffolds prepared under various conditions. It can be seen from Table 1 that the porosity and apparent density of porous scaffolds have nothing to do with the particle size of paraffin microspheres, but are greatly affected by the concentration of PLLA solution and the amount of wax balls. At a higher concentration of PLLA solution, the obtained porous scaffold has higher apparent density and lower porosity; the greater the amount of paraffin microspheres, the lower apparent density and higher porosity of the obtained scaffold. Figure 4 a. Figure 4 ...

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Abstract

The production process of porous polymer rack includes suspending dispersion to prepare paraffin microballoon, mixing paraffin microballoon and polymer solution, lowering temperature to make polymer solution produce phase separation, and freeze drying to eliminate solvent, boiled hexane washing or extracting to eliminate paraffin microballoon. The said process concentrates the advantages of bothpore creating process and thermal phase separation process and the produced porous polymer rack has macro pores with size determined by the paraffin microballoon as pore creating agent and micro pores produced in thermal phase separation and distributed on wall of the macro pores. The porous polymer rack has controllable pore size, good communication and high porosity.

Description

technical field [0001] The invention relates to a preparation method of a porous polymer scaffold for tissue engineering, in particular to a method for preparing a porous polymer scaffold for tissue engineering by combining wax ball porogen and thermal phase separation. Background technique [0002] The defect or failure of human tissue is a common disease, which seriously affects the patient's health and quality of life. One of the modern treatments for these diseases is tissue engineering technology. The main idea of ​​tissue engineering is to inoculate living cells with certain related functions on the extracellular matrix substitute, which can provide a spatial structure for cells to grow on. After a certain period of in vitro culture, a complex of cells and substitutes is formed, and then the resulting complex is transplanted to the damaged tissue in the body to repair the damaged tissue. The substitute materials used in tissue engineering are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/56A61L31/00C08J9/32C08L33/02
Inventor 高长有马祖伟龚逸鸿沈家骢
Owner ZHEJIANG UNIV
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