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An insect repellent isolated from foeniculum vulgare fruit

An insect repellent, fennel technology, applied in the direction of animal repellent, plant growth regulator, biocide, etc., can solve the problem of lack of repellent development and other issues

Inactive Publication Date: 2003-11-05
NATUROBIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite advances in the detection of non-toxic alternative plant materials, the development of repellents to address complex situations is still lacking

Method used

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  • An insect repellent isolated from foeniculum vulgare fruit
  • An insect repellent isolated from foeniculum vulgare fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Sample preparation and analysis of active compounds

[0038] 13 kg of fennel fruit purchased as a commercial product were dried in an oven at 60° C. for 3 days. The samples were crushed using a blender, and then extracted twice with 100 L of methanol at room temperature for two days. The extract was filtered using Toyo filter paper No2 (Japan), and after filtration, it was concentrated in vacuo at 35° C. to obtain a methanol extract of fennel fruit with a yield of 6%. The yield of methanol extract was calculated by the mass ratio of basic plant fennel fruit to methanol extract. The methanol extract of 780 g was subsequently separated using the polarity and density of the organic solvent, and then 20 g of the methanol extract was separated into n-hexane (18.6 g), chloroform (0.8 g), ethyl acetate (0.1 g), and water-soluble portion (0.5g).

[0039] Experiment 1

[0040] Determine the repelling activity of the methanol extract, hexa...

Embodiment 2

[0051] Separation of the active ingredient in the hexane fraction (720 g) with strong repelling activity

[0052] figure 1 A procedure for isolating the active components of hexane is shown.

[0053] first chromatography

[0054] Hexane chromatography was performed on a silica gel column (Berck 70-230 wells, 600 g, 5.5 i.d. x 70 cm) and gradient elution was performed using a hexane-ethyl acetate elution solution. The ratios of hexane and ethyl acetate used in the gradient solution were 90:10, 85:15, 73:30, 50:50 and 0:100, and a portion of each ratio was collected and named respectively: H1, H2, H3, H4, and H5. The patch test of Experiment 1 was used to test the repelling rate of the five substances to mosquitoes, and as a result, two parts H1 (187g) and H4 (57g) with repelling activity were separated.

[0055] second chromatography

[0056] The active ingredient isolated from the first silica gel chromatography was chromatographed.

[0057] First, H1 was eluted with...

Embodiment 3

[0072] Identification of Active Compounds

[0073] The structure of the active ingredient isolated from Example 2 was confirmed by spectroscopic analysis. Recorded by JNM-LA400F7 spectrometer (TMS as an internal standard) 1 H-and 13 C-NMR spectra and chemical conversions in ppm. Electron impact (EI) UV spectra were obtained from a UV spectrometer (KontronInstrument UVIKON 922) and mass spectra were obtained on a JEOLGSX400 spectrometer. The two active ingredients isolated from Example 2 were fennelone and E-9-octadecenoic acid represented by Chemical Formulas 1 and 2 below.

[0074] [chemical formula 1]

[0075] [chemical formula 2]

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Abstract

The present invention relates to an insect repellent isolated from the plant fennel fruit (Foeniculum vulgare fruit), in particular to an insect repellent comprising one or more selected from a group of fennel oil isolated from the plant fennel fruit, (+)-Feminone and E-9-octadecenoic acid compound. The fennel oil, (+)-feminone and E-9-octadecenoic acid of the present invention can be used as insect repellent components because they have no toxicity.

Description

technical field [0001] The present invention relates to an insect repellent isolated from the plant fennel fruit (Foeniculum vulgare fruit), in particular to an insect repellent comprising one or more selected from a group of fennel oil isolated from the plant fennel fruit, A compound composed of (+)-fenchone and E-9-octadecenoic acid. Background technique [0002] There are approximately 3000 different species of mosquitoes in the world, of which about 500 species of Aedes, about 300 species of Culex and about 350 species of Anopheles cause disease (Harwood, R.F. and M.T.James.1979, Entomology in Human and Animal Health. Macmillan). It is reported that there are about 51 species of mosquitoes in Korea, of which 20 are Aedes, 18 are Culex, and 7 are Anopheles (Hyo Suk You, 1994. Chack list of Insecta from Korea. Kon-Kuk University Press). [0003] Mosquitoes not only bite humans and animals, but also transmit mosquito-borne diseases such as malaria, encephalitis, yellow fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/00A01N35/06A01N37/06A01N65/10A01P7/00A61K8/35A61K8/36A61K8/97A61Q17/00A61Q17/02
CPCA01N35/06A01N37/06A61Q17/02Y10S424/10A01N65/10A61K8/9789Y02A50/30A01N65/32
Inventor 安龙峻金度亨金顺日
Owner NATUROBIOTECH
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