Method for oil desulfuration by using calcium alginate immobilized Diehliumyces pseudomonads R-8

A technology of Pseudomonas and calcium alginate, applied in the petroleum industry, refining hydrocarbon oil, fermentation, etc., can solve the problems of increased process complexity and low desulfurization efficiency, and achieve high activity of immobilized cells, low production cost, The effect of avoiding pollution

Inactive Publication Date: 2003-11-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although Naito et al. (Naito et al. Appl Microbiol. Biotechnol. 2001, 55: 374-378.) also used calcium alginate as an immobilized carrier, because it did not add water phase during the desulfurization reaction of the simulated oil system, and Calcium algi

Method used

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  • Method for oil desulfuration by using calcium alginate immobilized Diehliumyces pseudomonads R-8
  • Method for oil desulfuration by using calcium alginate immobilized Diehliumyces pseudomonads R-8
  • Method for oil desulfuration by using calcium alginate immobilized Diehliumyces pseudomonads R-8

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Cultivate the cells in the logarithmic growth phase of Pseudomonas delbrueckii R-8 whose accession number is CGMCC 0570:

[0035] Pick the Pseudomonas delbrueckii R-8 strain cultured on the vegetative slant, add it to a medium with a volume of 25 ml, and cultivate it for 2 days at 30°C and 170r / min, and then culture the Pseudomonas delbrueckii Inoculate the R-8 strain of Pseudomonas delbrueckii into a 500ml Erlenmeyer flask containing 150ml of the above-mentioned medium, culture it on a shaker until the logarithmic growth phase, and centrifuge at 5000rpm for 6min to obtain Pseudomonas delbrueckii R-8 cells; wash it with physiological saline 2 to 3 times, the obtained Pseudomonas delbrueckii R-8 cells were suspended in normal saline and placed in a refrigerator at 4°C; the composition of the medium was as follows: 1000ml deionized water, KH 2 PO 4 2.44g; Na 2 HPO 4 12H 2 O 12.03g; NH 4 Cl 2.0g; MgCl 2 ·6H 2 O 0.4g; CaCl 2 2H 2 O 0.001g; FeCl 3 ·6H 2 O 0.00...

Embodiment 2

[0045] 1. Cultivate the quiescent cells of Pseudomonas delbrueckii R-8 whose accession number is CGMCC 0570:

[0046] Take 0.5 milliliters of glycerol to preserve Pseudomonas delbrueckii R-8 and add it to a medium with a volume of 50 milliliters, cultivate it for 2 days at 30°C and 170 r / min, and then culture the Pseudomonas delbrueckii R-8 -8 strain was inoculated into a 500ml Erlenmeyer flask containing 150ml of the above-mentioned medium, cultured on a shaking table for 2 days, transferred to a fermenter for cultivation to a stationary phase, and was centrifuged at 5000rpm for 6min to obtain Pseudomonas delbrueckii R-8 thalline; Wash with normal saline for 2 to 3 times, suspend the Pseudomonas delbruecki R-8 cells obtained in normal saline, and place them in a refrigerator at 4°C;

[0047] The composition of the medium is as follows: deionized water 1000ml, KH 2 PO 4 2.44g; Na 2 HPO 4 12H 2 O 12.03g; NH 4 Cl 2.0g; MgCl 2 ·6H 2 O 0.4g; CaCl 2 2H 2 O 0.001g; FeCl ...

Embodiment 3

[0057] Repeatedly using the calcium alginate immobilized cells after activation of the proliferation culture obtained in Example 1 to remove sulfur in dibenzothiophene (DBT) in simulated oil (dodecane):

[0058] Take several grams of the calcium alginate immobilized cells (containing 0.2g stem cells) obtained after the proliferation culture activation prepared in Embodiment 1 in a 100ml Erlenmeyer flask, add 5ml proliferation medium and 5ml dodecane (dissolved 1mmol / LDBT), The volume of the water phase accounts for 50% of the total volume of the oil-water phase. React on a shaking table at 30°C and 170r / min. After 24 hours of reaction, pour off the oil-water phase, wash the immobilized cells with normal saline for 3 times, and then add 5ml of fresh proliferation medium and 5ml of dodecane (dissolved 1mmol / L DBT); at 30°C, 170r / min conditions, carry out shaking table reaction; use HPLC to analyze the content of DBT in the oil phase; after 24 hours, repeat the above process, and ...

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Abstract

The method of utilizing calcium alginate immobilized Diehliumyces pseudomnad R-8 in desulfurizing oil includes the following steps: preparing Diehliumyces pseudomnads R-8 of preservation number CGMCC0570 cell in logarithmic growth phase or quiescent phase; embedding the obtained Diehliumyces pseudomnads R-8 thallus with calcium alginate; amplifying and culturing the obtained immobilized cells, desulfurizing oil repeatedly. The said method has high activity of immobilized cells, high stability, easy recovering of biological catalyst for reuse, no pollution to oil product by the pigment produced in the desulfurization with free cell, and cheap and biocompatible calcium alginate used, simple technological process and low production cost, and may be used in industrial production easily.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for desulfurizing oil products by immobilizing Pseudomonas delbrueckii R-8 with calcium alginate. Background technique [0002] The sulfide contained in petroleum and its products produces sulfur dioxide when burned, which causes environmental pollution and is one of the main reasons for the formation of acid rain, which poses a threat to the sustainable development of human beings. At the same time, the presence of sulfide will also affect the appearance of fuel and its products, and corrode the transportation equipment. To this end, the sulfides in petroleum and its products must be removed. At present, the developed countries in the world have formulated strict regulations to control the sulfur content in petroleum products. my country's SO 2 emissions far exceed the world average. Faced with the increasingly severe environmental...

Claims

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Application Information

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IPC IPC(8): C10G31/00C12P5/00
Inventor 罗明芳刘会洲邢建民缑仲轩李珊陈家镛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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