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Method for quantitatively analyzing kojic dipalmitate and use thereof

A technology for quantitative analysis of kojic acid dipalmitate, applied in the field of high performance liquid chromatography analysis

Inactive Publication Date: 2004-02-04
SHANGHAI JAHWA UNITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on the quantitative determination method of kojic acid fatty acid derivatives

Method used

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  • Method for quantitatively analyzing kojic dipalmitate and use thereof
  • Method for quantitatively analyzing kojic dipalmitate and use thereof
  • Method for quantitatively analyzing kojic dipalmitate and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The determination of embodiment 1 HPLC analysis condition

[0027] This embodiment adopts Waters high-performance liquid chromatography system: Alliance 2690 pump, YWG C18 250×4.6mm 10 μ m column, 2487 ultraviolet / visible light detector, Millennium 32 software system; reagents: methanol and tetrahydrofuran are dedicated to HPLC, water is distilled— Deionized water.

[0028] (1) Selection of mobile phase

[0029] Using different ratios of tetrahydrofuran / methanol as mobile phase to make kojic acid dipalmitate chromatographic analysis, obtain a group of spectrograms ( figure 1 ). It can be seen that when the ratio of tetrahydrofuran / methanol is from 45 / 55 to 15 / 85, better separation can be achieved, while the retention time of the chromatographic peak gradually moves backward with the increase of the ratio of methanol, and the degree of separation from the impurity peak also gradually increases. The separation effect is best when the ratio of THF / methanol is 25 / 75. If...

Embodiment 2

[0038] Example 2 Quantitative correlation experiment

[0039] A group of kojic acid dipalmitate standard series samples with different contents are used for chromatographic analysis respectively according to the above analysis conditions, and the obtained chromatographic peak area and content are used for linear regression analysis, and the obtained regression curve has a good linear relationship ( Figure 4 ), correlation coefficient r=0.9995.

Embodiment 3

[0040] Embodiment 3 recovery rate and repeatability experiment

[0041] Add kojic acid dipalmitate to two skin creams that do not contain kojic acid dipalmitate, so that the content reference values ​​are 10.0 and 100 μg / g, respectively, and after mixing thoroughly, perform parallel measurements 10 times according to this method. The results are shown in the table 1.

[0042] Table 1. Method recovery rate and repeatability test results

[0043] Determination Standard Average Average Standard Relative

[0044] Sample number Number of times Addition amount Recovery amount Recovery rate Deviation Standard deviation

[0045]Times μg / g μg / g % SD RSD%

[0046] 1 10 10 9.89 98.9 1.8 1.8

[0047] 2 10 100 99.6 99.6 1.31 1.3

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Abstract

The sample can be analysed by the method with utilizing chromatographic principle of reversed phase partition under condition of column temperature 25-45 degree C, mobile phase oxolanel / methanel=45 / 55 to 15 / 85, flow velocity 0.8-1.5 ml / minute and detection wavelength 250 nm.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography (HPLC) analysis method, in particular to a method for quantitatively analyzing kojic acid dipalmitate by HPLC. Background technique [0002] Kojic acid dipalmitate is a derivative of kojic acid fatty acid, which has recently been considered as an effective and relatively stable skin whitening agent. received considerable attention. In particular, the research on the transdermal penetration behavior of kojic acid fatty acid derivatives can provide a basis for the rationality of the formula in the development of whitening products, and accurate determination of the content of kojic acid fatty acid derivatives is one of the necessary means for these research work. In particular, the determination of trace amounts of kojic acid fatty acid derivatives in biological samples such as skin extracts requires a method with high precision and high sensitivity. However, there is no relevant report ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36G01N30/40
Inventor 蔡惠民周莉
Owner SHANGHAI JAHWA UNITED
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