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Preparation method for tumour cell comprising interleukin-23 gene and its use

A technology of interleukin and tumor cells, which is applied in the field of preparation of tumor cells and can solve the problems of not obtaining clinical curative effect and the like

Inactive Publication Date: 2004-03-17
单保恩
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The treatment of tumors with recombinant cytokines such as interleukin-12, interleukin-2, IFN-α, IFN-γ, and TFN-α has shown certain curative effects, but it is still in the stage of clinical trials and has not yet achieved clinical efficacy the result of

Method used

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  • Preparation method for tumour cell comprising interleukin-23 gene and its use
  • Preparation method for tumour cell comprising interleukin-23 gene and its use
  • Preparation method for tumour cell comprising interleukin-23 gene and its use

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Embodiment Construction

[0038] The embodiments of the present invention will be further described below in conjunction with the accompanying drawings:

[0039] The overall idea of ​​this embodiment is as follows:

[0040] The vector is retrovirus.

[0041] Using retrovirus as a vector to introduce interleukin-23 into tumor cells includes the following steps:

[0042] (1) Using guanidine isothionate one-step method to extract total tissue RNA from mouse spleen cells;

[0043] (2) Obtain mouse interleukin-23p19 and p40 cDNA by reverse transcription polymerase chain reaction method, insert the obtained cDNA into the Pcr2.1 vector in the TA Cloning kit, and perform parallel polymerase chain reaction with a DNA sequencer. Sequencing to verify the correctness of the cDNA; insert the verified cDNA into the viral vector with restriction endonuclease and DNA ligase;

[0044] (3) Use a kit (product of Invitrogen, USA) to introduce the viral vector obtained in step (2) into packaging cells, which can package the vi...

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Abstract

The present invention belongs to the preparation process of transgenic tumor cell for treating malignant tumor, and is especially the preparation process of tumor cell containing interleukin-23 transgene. The tumor treating method of the present invention is superior to available tumor treating methods. Interleukin-23 gene is introduced via carrier into tumor cell to produce interleukin-23 withpowerful tumor resisting effect to treat malignant tumor. It functions in the initial immune response stage and activate dendritic cell to induce natural killing of cell. Interleukin-23 can also induce the proliferation and activation of memory T cell, secret IFN-gamma, regulate immunological function, inhibit the vascularization in tumor part and thus reach the effect of curing malignant tumor.

Description

Technical field [0001] The invention belongs to a method for preparing transgenic tumor cells for treating malignant tumors, in particular to a method for preparing tumor cells transfected with interleukin-23 gene. Background technique [0002] At present, the main treatment methods for tumors include surgical resection, radiotherapy and chemotherapy. These treatment methods have certain side effects on the body and do not have long-term prognostic improvement effects. For example, chemotherapy and radiotherapy can often inhibit bone marrow hematopoiesis and the body Immune function, leading to prone to infection and low anti-tumor immune response; surgical trauma can also inhibit various immune functions of the body, and there is a danger of expanding micrometastases. Direct use of cytokines to treat tumors has achieved certain curative effects. However, the half-life of cytokines in the body is short, it is difficult to maintain a certain concentration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00C12N5/00C12N15/86C12N15/88
Inventor 田川雅敏单保恩
Owner 单保恩
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