Method for widening the cell hereditable basic of cultivated soybeans using wild soybeans
A technology for cultivating soybeans and wild soybeans, which is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement to achieve the effect of broadening the genetic basis and stabilizing beneficial traits
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[0052] Example 1
[0053] (1) Obtain F1 true hybrids with wild soybean as the female parent
[0054] In 1984, the high-protein Guxin wild soybeans were planted and the flowering period was observed. The Guxin wild soybeans from southern Hebei showed late maturity in Chengde, northern Hebei, and began to bloom on August 20, and all spring soybeans All the varieties have bloomed, and the pollination and hybridization work has not been completed. It was sown again in 1985, and 20 days of short-day treatment was carried out after the emergence of the seedlings. It was shaded from 5 pm to 8 o’clock the next day, so that it bloomed on June 23, which was 57 days earlier than natural conditions, creating a hybrid condition. In order to reduce the plant height and increase the high yield of the offspring of interspecific hybrids, a limited plant height of 60 cm and good yield of Chengdou No. 1 was selected as the male parent for crosses. One F0 seed was obtained after autumn.
[0055] In t...
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[0059] Example 2
[0060] In order to verify the reproducibility of the successful hybridization of wild soybeans as female parent and cultivated soybean, Kongqiao wild soybeans were used as female parent in 1994. After 18 days of short-day treatment, after flowering, henan soybeans with limited size and large grains were used as male parent. The hybridization was successful again. The combination number 9441, F1 and F2 were all crossed in a broad sense. The F3 substitute Zhongpin 661 was also crossed in a broad sense to obtain 5 F0 seeds, the combination number 9718. In the future, 9718F4 and 9441-7 will be used as female parent, and multiple cultivated soybeans such as Yudou 18, Chengdou 5, Zhongpin 5016, Yudou 12, Wuyi Yan Zhongying soybeans, Jiqing No. 1, 9039-1-8= The 1st class has carried out generalized hybridization work and has obtained a batch (204 in 10 combinations) of interspecific hybrid progeny materials with thick stems, large grains and wild soybean cytoplasm. Thi...
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[0061] Example 3
[0062]In order to broaden the genetic basis of cultivated soybean cytoplasm and suggest the reason and mechanism of the narrow and single cytoplasmic genetic basis of cultivated soybean in my country, in 1995, wild soybean Xinmin 6 was used as the parent, and the four cultivated soybean Hefeng 25 and Tongnong 10 were used as parents. For 8412 and Kai 8157 soybeans, 4 pairs of cross-crossing experiments were carried out. From 1996 to 1998, the separation of main agronomic traits and maternal effects of the F1, F2, and F3 generations of cross-crossing experiments were carefully observed and analyzed.
[0063] The test results show that: plant height has a very significant maternal effect (up to 0.01% significant). The number of main stem nodes and the number of pods per plant also have maternal effects. The number of seeds per plant has a significant maternal effect, reaching 0.05% significant. The last pair of 100-seed weights shows maternal effect. The first 3 p...
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