Method for parameter control of the process for culturing serum-suspension free animal cell

A technology of animal cells and suspension culture, applied in the field of bioengineering, can solve the problems of toxic waste accumulation and other problems, and achieve the effects of good stability, strong controllability and small difference in results

Inactive Publication Date: 2004-12-29
陈志南
View PDF4 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0035] 2. Accumulation of toxic waste

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for parameter control of the process for culturing serum-suspension free animal cell
  • Method for parameter control of the process for culturing serum-suspension free animal cell
  • Method for parameter control of the process for culturing serum-suspension free animal cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0074] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments given by the inventor.

[0075] According to the technical scheme of the present invention, the applicant uses the hybridoma cell HAb18, which is a BALB / c mouse immunized with fresh human liver cancer tissue cells, and takes its immune spleen cells to fuse with the mouse myeloma cell SP2 / 0 to obtain HAb18 Hybridoma cells, the cell line can stably and efficiently express monoclonal antibodies against human liver cancer-related antigens (preserved by the General Microorganism Collection Center of the China Microorganism Culture Collection Management Committee, preservation number: CGMCC No 0426).

[0076] Use a mechanically stirred bioreactor to culture hybridoma cells in large-scale high-density suspension; use expanded column bed technology to recover and purify monoclonal antibodies in large-scale cell culture medium in one step, and per...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The technological process includes feeding concentrated energy source matter fluid and amino acid, and perfusing basic culture medium. The process parameter control based on the growth and metabolism requirement of specific cell includes setting upper and lower limit indexes of glutamine, glucose, ammonia and lactic acid, controlling the feeding rate of glutamine, glucose, amino acid and other nutritive matter, and perfusing serum-free basic culture medium to lower the density of metabolic product. The cell growth state is monitored via detecting oxygen intaking rate, and the feeding rate is controlled precisely with computer program controlled feeding system. The technology of the present invention is especially suitable for large scale culture of hybrid tumor cell for producing treating antibody, and has the features o high stability, high control ability, determined control indexes, etc.

Description

technical field [0001] The invention belongs to the cell engineering technology in the technical field of bioengineering, and mainly relates to the large-scale cultivation of animal cells (engineering cells such as hybridoma, CHO, SP2 / 0, NSO, etc.) and the process for producing recombinant protein therapeutic drugs, and specifically relates to animal cell serum-free A method for controlling parameters of a suspension culture process. Background technique [0002] The large-scale cultivation of animal cells has become one of the most important key technologies in the field of biopharmaceuticals, and the in-depth and progress of its research has promoted the rapid development of the biotechnology industry. At present, the main method of industrial production of animal cells expressing recombinant protein therapeutic drugs is to cultivate hybridoma cells and engineered cells (CHO, CHO, SP2 / 0, NSO), insect cells and microorganisms, etc., and then concentrated and purified by re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/16C12P21/00
Inventor 米力冯强李玲王贤辉
Owner 陈志南
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap