Method for parameter control of the process for culturing serum-suspension free animal cell

A technology of animal cells and suspension culture, applied in the field of bioengineering, can solve the problems of toxic waste accumulation and other problems, and achieve the effects of good stability, strong controllability and small difference in results
CN1557948AInactive Publication Date: 2004-12-29้™ˆๅฟ—ๅ—
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
้™ˆๅฟ—ๅ—
Publication Date
2004-12-29
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The technological process includes feeding concentrated energy source matter fluid and amino acid, and perfusing basic culture medium. The process parameter control based on the growth and metabolism requirement of specific cell includes setting upper and lower limit indexes of glutamine, glucose, ammonia and lactic acid, controlling the feeding rate of glutamine, glucose, amino acid and other nutritive matter, and perfusing serum-free basic culture medium to lower the density of metabolic product. The cell growth state is monitored via detecting oxygen intaking rate, and the feeding rate is controlled precisely with computer program controlled feeding system. The technology of the present invention is especially suitable for large scale culture of hybrid tumor cell for producing treating antibody, and has the features o high stability, high control ability, determined control indexes, etc.
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Description

technical field

[0001] The invention belongs to the cell engineering technology in the technical field of bioengineering, and mainly relates to the large-scale cultivation of animal cells (engineering cells such as hybridoma, CHO, SP2 / 0, NSO, etc.) and the process for producing recombinant protein therapeutic drugs, and specifically relates to animal cell serum-free A method for controlling parameters of a suspension culture process. Background technique

[0002] The large-scale cultivation of animal cells has become one of the most important key technologies in the field of biopharmaceuticals, and the in-depth and progress of its research has promoted the rapid development of the biotechnology industry. At present, the main method of industrial production of animal cells expressing recombinant protein therapeutic drugs is to cultivate hybridoma cells and engineered cells (CHO, CHO, SP2 / 0, NSO), insect cells and microorganisms, etc., and then concentrated and purified by re...

Claims

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