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Separation analyzing method for lipophilicity extraction in agricultural waste

A technology for separation and analysis of agricultural wastes is applied in the field of separation and analysis of lipophilic extracts in agricultural wastes to achieve the effects of saving usage, short time and few steps.

Inactive Publication Date: 2005-01-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the reports on the chemical composition of wheat straw extract mainly focus on the strong polar components containing phenols, while there are few studies on the composition of lipophilic extracts, only some qualitative research reports on free fatty acids and sterols

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Dried wheat stalks were cut into small pieces, ground, and the part that passed through the 4mm sieve was taken, and placed in an oven at 45°C to keep the air circulation dry for 15 hours. Take 40g and use 1400ml organic solvent in a Soxhlet extractor. Methyl-tert-butyl ether was extracted for 12 h, and the solvent was removed with a rotary evaporator at 30 °C, and the residue was placed in N 2 Dry and weigh in the flow, calculate the yield of extract;

[0021] (2) 2 mg of the dried lipophilic extract was silylated with 80 μl bistrimethylsilyltrifluoroacetamide and 40 μl trimethylchlorosilane, and the reaction was carried out in a closed container, and the reaction was kept at 75°C 20min, cool, add 120μl toluene, shake well, and use for gas chromatography analysis;

[0022] (3) The silanized sample is analyzed by gas chromatography. The gas chromatograph adopts Rtx-1 capillary column, which is filled with cross-linked 100% polydimethylsiloxane. 0.10μm, with a flam...

Embodiment 2

[0024] (1) After drying the barley straw, cut it into small pieces, grind it, take the part that passed through the 4.75mm sieve, put it in an oven at 50°C to keep the air circulation dry for 22 hours, take 60g and use 2200ml organic The solvent methyl-tert-butyl ether was extracted for 15 h, and the solvent was removed with a rotary evaporator at 35 °C, and the residue was placed in N 2 Dry and weigh in the flow, calculate the yield of extract;

[0025] (2) 3 mg of the dried lipophilic extract was silylated with 120 μl of bistrimethylsilyl trifluoroacetamide and 60 μl of trimethylchlorosilane, and the reaction was carried out in a closed container, and the reaction was kept at 75° C. 20min, cool, add 150μl toluene, shake well, and use for gas chromatography analysis;

[0026] (3) The silanized sample is analyzed by gas chromatography. The gas chromatograph adopts Rtx-1 capillary column, which is filled with cross-linked 100% polydimethylsiloxane. 0.10μm, with a flame ioniza...

Embodiment 3

[0028] (1) Dried rye straw is cut into small pieces, ground, and the part that passes through a 5mm sieve is taken, placed in an oven at 50°C to keep the air circulation dry for 20 hours, and 50g is used in a Soxhlet extractor with 1800ml organic The solvent methyl-tert-butyl ether (MTBE) was extracted for 14h, and the solvent was removed with a rotary evaporator at 35°C, and the residue was placed in N 2 Dry and weigh in the flow, calculate the yield of extract;

[0029] (2) 3 mg of the dried lipophilic extract was silylated with 120 μl of bistrimethylsilyl trifluoroacetamide and 60 μl of trimethylchlorosilane, and the reaction was carried out in a closed container, and the reaction was kept at 75° C. 25min, cool, add 200μl toluene, shake well, and use for gas chromatography analysis;

[0030](3) The silanized sample is analyzed by gas chromatography. The gas chromatograph adopts Rtx-1 capillary column, which is filled with cross-linked 100% polydimethylsiloxane. 0.10μm, wi...

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Abstract

The invention discloses a separating and analyzing method for lipophilic extracted substances from the agricultural waste, introduces a method using double-trimethyl silicane group fluoroform acetamide and trimethyl chlorosilane to carry on methyl silicane process after the lipophilic extracted substances is extracted through organic solvent, then using high temperature Rtx-1 capillary to carry on gas phase chromatogram analysis, determining the content and composition of the lipophiltic extracted substances.

Description

technical field [0001] The invention belongs to the technical field of qualitative and quantitative determination of lipids, in particular to a method for separation and analysis of lipophilic extracts from agricultural waste. Background technique [0002] Lipophilic extract refers to substances obtained by extracting wood or pulp with organic solvents such as ethanol, acetone, or methylene chloride. In general, extractives account for 3 to 6% of the weight of the wood. Non-polar extracts, usually called resins, are mainly composed of fatty acids, resin acids, fatty acid esters (such as sterol esters, waxes and triglycerides), and neutral compounds such as fatty alcohols and sterols. In addition to lipophilic extracts, there are also hydrophilic extracts in wood, such as lignans. The chemical composition and content of the extract are affected by many factors, such as tree species, tree age and different parts of the tree. Lipophilic extracts are...

Claims

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Application Information

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IPC IPC(8): G01N30/30
Inventor 孙润仓孙晓锋许凤许传富
Owner SOUTH CHINA UNIV OF TECH
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