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Mineralized composite material of gene recombination collagen and preparation method

A technology of gene recombination and composite materials, applied in the field of bioengineering, can solve the problems of viruses and hidden dangers, and achieve the effect of low immune rejection, good biocompatibility, and no virus hidden dangers.

Inactive Publication Date: 2005-01-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the currently used collagen is mainly derived from animals, there are hidden dangers of viruses, and the recombinant collagen produced by genetic engineering methods can avoid this problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Add 500ml of Ca(OH) with a concentration of 0.25mol / L dropwise in the 500ml recombinant collagen solution with a concentration of 0.9%. 2 After solution, continue to slowly add NaH with a concentration of 0.25mol / L 2 PO 4 Solution 300ml, mix evenly with a magnetic stirrer while adding dropwise.

[0025] 2) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a freeze dryer to freeze dry, and then grind the dried product to make Get dry powder.

Embodiment 2

[0027] 1) Add 25ml of CaCl with a concentration of 0.2mol / L to the 450ml recombinant collagen solution with a concentration of 33%. 2 After the solution, continue to slowly drop Na with a concentration of 0.2mol / L 2 HPO 4 Solution 25ml, mix evenly with a magnetic stirrer while adding dropwise.

[0028] 2) Slowly add NaOH solution dropwise to the solution in the first step above while stirring until the pH value is stable at 7.4.

[0029] 3) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer for freeze drying, and then grind the dried product Get dry powder.

Embodiment 3

[0031] 1) In the adjusted 100ml recombinant collagen solution with a concentration of 10%, add KH with a concentration of 2mol / L 2 PO 4 solution 500ml, continue to slowly add 400ml concentration of 5mol / L Ca(NO 3 ) 2 After the solution was added dropwise, it was mixed evenly with a magnetic stirrer.

[0032] 2) Slowly add KOH solution dropwise to the solution in the first step above while stirring until the pH value is 8.0.

[0033] 3) Stir the above system, let it stand, remove the supernatant, centrifuge to separate the precipitate, wash it repeatedly with deionized water to remove the salt and reach neutrality, put it into a lyophilizer for freeze drying, and then grind the dried product Get dry powder.

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PUM

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Abstract

A mineralized genetically recombinant collagen composition for repairing bone is prepared through preparing the solution of genetically recombinant collagen in acid or water, dropping Ca ions contained solution in it while stirring, adding PO4 ions contained solution, regulating pH value, stirring, laying aside, centrifugal separation and washing several times, and freeze drying. It has high biocompatibility.

Description

technical field [0001] The invention relates to a mineralized gene recombinant collagen composite material and a preparation method thereof, belonging to the technical field of bioengineering. Background technique [0002] Collagen is an extremely important structural protein of connective tissue, which supports organs and protects the body. Collagen is the most abundant protein in the human body, accounting for 25%-30% of the total protein in the body, equivalent to 6% of body weight. Collagen products are widely used in medicine, beauty, cosmetics and other industries, but at present, no matter at home or abroad, their production uses animal skin, Achilles tendon, cartilage and human placenta extracts. The collagen extracted by these methods has hidden dangers of viruses and immune reactions. or ethical issues. With the development of genetic engineering, gene recombinant expression technology has been applied to the production of recombinant collagen. Recombinant colla...

Claims

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Application Information

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IPC IPC(8): A61L27/44
Inventor 崔福斋王玉翟勇
Owner TSINGHUA UNIV
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