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Cholesterin test strip of electrochemical sensor and prepn. process of used reagent

A cholesterol and electrochemical technology, applied in the field of biosensors, can solve the problems of uneven electrode strip surface, complex process, time-consuming, etc., achieve high sensitivity, accurate detection results, and reduce production costs

Inactive Publication Date: 2005-01-12
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

[0003] At present, most of the single-parameter disposable sensor test strips and detection instruments in the market rely on the thick film technology of screen printing. The production of sensor test strips using thick film technology is time-consuming and consumable. The electrodes obtained by the screen printing method The surface of the belt is very uneven, the process is more complicated, and it is not as good as the thin film electrode based on the advanced microelectromechanical system (MEMS) manufacturing technology in terms of response time, sensitivity, stability, etc.

Method used

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  • Cholesterin test strip of electrochemical sensor and prepn. process of used reagent
  • Cholesterin test strip of electrochemical sensor and prepn. process of used reagent
  • Cholesterin test strip of electrochemical sensor and prepn. process of used reagent

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Embodiment 1

[0041] figure 1 and figure 2 It is a two-electrode diagram designed by the present invention. First, the thin-film gold electrodes 2 and 3 are prepared on the insulating substrate 1 by micro-electro-mechanical system (MEMS) technology, and the second insulating layer 4 is adhered on the thin-film gold electrodes 2 and 3, and the second insulating layer 4 is provided with Capillary channel 7, which forms a reaction zone. Then, the nano-platinum black layer 8 is assembled in the capillary action channel 7 by electrodeposition. The method refers to the applied patent 03141154.1. After electrodeposition, wash the electrodes 2, 3 and the surface of the nano-platinum black layer 8 with a large amount of deionized water to remove Various ions remaining on the surface of the electrodes 2, 3 and the nano-platinum black layer 8 were dried for 10 minutes in an electric blast drying oven at 37°C, and taken out for storage for later use. Add reagent layer 9: Dissolve 8.2 mg of potassiu...

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Abstract

A test bar is prepared from following steps: preparing gold film electrode on insulating material by using micro electric mechanical system (MEMS); after electrode is treated through surface activation technique, a layer of nano metal grain is assembled on reaction zone on surface of the electrode through method of electrochemical deposition; then, reaction reagent is fixed on the said layer of nano metal grain so as to form reagent layer. When blood cholesterol of object to be analyzed through capillary action adds in drop wise to reaction zone of the test bar, detection system of electrochemical biosensor detects concentration of total cholesterol rapidly.

Description

technical field [0001] The invention relates to the technical field of biosensors, and relates to a current type biosensor rapid detection total cholesterol test strip and a manufacturing method of the reagent used. The test strip can quickly detect the concentration of total cholesterol. technical background [0002] Diabetes is one of the five diseases with the highest morbidity and mortality in the world. Diabetic patients are often accompanied by lipid metabolism disorders to increase blood lipids, and hyperlipidemia (total cholesterol is an important indicator) is an important factor that causes hypertension and atherosclerosis. Cardiovascular disease is the second leading cause of death in my country, and the incidence and mortality of coronary heart disease are rising in some areas. Available evidence suggests that several risk factors play an important role in the pathogenesis of CHD, including hypercholesterolemia, hypertension, smoking, and diabetes. Low high-den...

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Application Information

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IPC IPC(8): A61K38/43G01N27/327
Inventor 刘春秀蔡新霞李华清姜利英罗贤波
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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