Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pineapple anhydride compound and its use

A technology of pineapple acid glycosides and compounds, applied in steroids, organic chemistry, drug combination and other directions, can solve the problem of rare pineapple leaves

Inactive Publication Date: 2005-01-26
TSINGHUA UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The records of pineapple leaves as medicine are relatively rare, especially the effects of pineapple leaves or bromelain and its new compound bromelain in treating diseases such as excessive peroxides in the body and cerebral ischemia have not been reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pineapple anhydride compound and its use
  • Pineapple anhydride compound and its use
  • Pineapple anhydride compound and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Isolation and preparation of bromelain A and its physicochemical and spectral data:

[0113] When preparing to have above-mentioned novel compound, at first pineapple leaf is heated and extracted 3 times at 60 ℃ with the alcohol of 50% concentration of 5 times of material weight, each time is 1 hour, obtains extract; Then extract is decompressed (pressure 98Kpa), the temperature was concentrated at 50°C, dissolved in water twice the weight of the material, centrifuged, and the precipitate was discarded. The supernatant was eluted with water through the macroporous adsorption resin, and the eluent was discarded, and then washed with 80% ethanol The eluate was concentrated under reduced pressure at a temperature of 50°C (pressure 98Kpa), and the concentrate was dried under reduced pressure (pressure 98Kpa) to obtain pineapple leaf extract. The pineapple leaf extract was dissolved in 70% ethanol, then mixed with silica gel, separated by silica gel column chromatography, el...

Embodiment 2

[0159] Isolation and preparation of bromelain B and its physicochemical and spectral data:

[0160] When preparing the above-mentioned new compound, the extract is then concentrated under reduced pressure (pressure 98Kpa) at 70°C, dissolved in water twice the weight of the material, centrifuged, and the precipitate is discarded, and the supernatant passes through the macroporous adsorption resin. First eluted with water, the eluent was discarded, and then eluted with 80% ethanol, the eluent was concentrated under reduced pressure at a temperature of 70°C (pressure 98Kpa), and the concentrate was dried under reduced pressure (pressure 98Kpa) to obtain pineapple leaf extract. The pineapple leaf extract was dissolved in 50% ethanol, then mixed with silica gel, separated by silica gel column chromatography, eluted with chloroform: acetone gradient, first separated by reversed-phase silica gel, followed by Sephadex purification, followed by bromelain After A, bromelain B is obtaine...

Embodiment 3

[0207] Example 3: Effects on Mice Meningeal Blood Flow

[0208] Observe the effect of bromelain A and B of the present invention on the meningeal blood flow of mice. Bromelain A, B. When used, it is prepared to the required concentration with physiological saline. The experimental animals are male ICR mice, non-inbred closed group, weighing 18-22 g, provided by Beijing Weitong Lihua Experimental Animal Center. The room temperature in the laboratory is 20-22°C, the relative humidity is 40%-60%, the ventilator is ventilated, the natural light source is 12h / day, and the cages are kept with 5 animals per cage. The cages are cleaned every three days.

[0209] Take mouse urethane (1.0g / kg) after anesthesia, fix the head, incise the skin, and place in the cerebral cortex motor area (AP -1 , L 2 ) placed a laser probe (JI-200 laser microcirculator, produced by Tianjin Nankai University), and after 5-10 minutes of stabilization, the cerebral blood flow was tested, and the changes o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the pineapple anhydride compound and its use, wherein the pineapple anhydride compound is extracted from pineapple leaves, which has proved functions of oxidation resistance and enhancing brain blood flow, thus can be used for treating apoplexy and grease metabolic disorder.

Description

technical field [0001] The present invention relates to a group of novel compound bromelain and its use in medical treatment, specifically the novel compound bromelain isolated and prepared from pineapple leaves, and the compound is used in the preparation and treatment of elevated peroxide, oxidation The application of various diseases caused by elevated low-density and high-density lipoproteins. Background technique [0002] Pineapple is the fruit of Ananas comosus (L.) Merr.[Bromelia comosa L.; A.sativusSchult.Et Schult.f.], also known as pineapple. It is native to the Americas and is now widely planted in tropical regions. It is planted in Fujian, Guangdong, Hainan, Guangxi, Yunnan and other places in my country. This product was first recorded in Qing Wu Qirui's "Plant Names and Facts", named after Ludouzi. The book records: "Pandouzi is produced in Guangdong. It is named Polo. It grows in the mountains and is like a radish. There are clusters of leaves on it, with lo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P25/00A61P43/00C07J53/00
Inventor 王伟杜力军丁怡邢东明王如峰马超王雪莉陈芸芸
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com