Biochemical detection liquid for urine two-gallbladder (bilirubin and urobilinogen)

A technology for biochemical detection and urinary bilirubin, applied in biological testing, analysis through chemical reactions of materials, material inspection products, etc., can solve problems that have not been done before, and achieve the effect of strong versatility and social popularization

Inactive Publication Date: 2005-01-26
赵青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, for the detection of urinary bicholesterol (urinary bilirubin, urobilinogen),

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of biochemical test solution for urine bicholesterol (urine bilirubin, urobilinogen)

[0033] Process requirements:

[0034] Urinary bilirubin biochemical detection solution: add trichloroacetic acid and glacial acetic acid to dissolve a little, add acidic ferric chloride to dissolve completely, add distilled water to the total demand.

[0035] The obtained product is a slightly yellow solution, with a pH of 10.4±0.2 and an absorbance of 0.2-0.6A at 415 nm. Store in a brown bottle sealed away from light at room temperature.

[0036] Urinary bile origin chemical detection solution: mix and dissolve concentrated hydrochloric acid and dimethylaminobenzaldehyde, pour the supernatant into the mixture of glacial acetic acid and distilled water, and mix and dissolve the two solutions.

[0037] The obtained product is a light yellow solution, with a pH of 0.2±0.2 and an absorbance of 0.8-1.5A at 415 nm. Store in a brown bottle sea...

Embodiment 2

[0039] Sensitivity of urine bilirubin biochemical detection solution

[0040] Aspirate 2 ml of bile and dissolve it in 100 ml of distilled water to prepare a 2v / v% bile solution. Prepare 1v / v%, 0.5v / v%, 0.25v / v%, 0.125v / v% mixed simulated bilirubinuria urine according to 2-fold serial dilution, add 0.5ml each into 4 transparent test tubes, and mark concentration.

[0041] registered concentration

[0042] The above experiments show that the sensitivity of the urine bilirubin biochemical detection solution can reach about 0.25-0.125%.

[0043] Sensitivity of urine bile primary chemical detection solution

[0044] Take water-soluble sulfonamides and dilute with distilled water to 8mg%, 4mg%, 2mg%, 1mg%, 0.5mg%, 0.25mg%, 0.12mg%, 0.06mg%, add 0.5ml each to 8 transparent test tubes, and indicate the concentration.

[0045] Detection concentration range

[0046] The above experiments show that the sensitivity of the urin...

Embodiment 3

[0048] urine bilirubin test

[0049] Detection concentration range

[0050]Normal urine should have no bilirubin, which is a negative result; any urine that is tested to be blue-green, light blue-green, light blue-green, or slightly blue-green is a positive result. According to the above results, the biochemical qualitative detection of urinary bilirubin is completely consistent with the classical method.

[0051] Urobilinogen test

[0052] Detection solution concentration range

[0053] 1:20 cherry red is supernormal. It is not normal for urobilinogen to be reduced or absent if it does not show light red and shows the original urine color. According to the above results, the primary chemical detection of urine and bile is completely consistent with the classical method.

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PUM

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Abstract

This is a biochemistry detection liquid of something including the two things (bilirubin and urobilinogen). The bilirubin comprises 0.89-1.2w/v percents acidic iron trichloride, 20-25.3w/v percents trichloroacetic acid, 5 percents waterless glacial acetic acid and distilled water. The urobilinogen comprises 1.8-2.2w/v percents paradimethy-laminobenzaldehyde, 20 percents dense hydrochloric acid 5 percents waterless glacial acetic acid and distilled water. This invention offers biochemistry detection liquid and self-checking method of bilirubin and urobilinogen for the mass scoutting and checking dynamically. Through the recording curve diagram in accordance with series detecting color result one can self-judge something: 1, warning signal of health and pathogenic factor; 2, forepart detectable rate of hepatopathy; 3. the synchronous tracking monitoring of curative effect during the treating course. The above liquid offer the likelihood of carrying out all this function.

Description

technical field [0001] The present invention relates to a urine biochemical detection solution based on urinary bicholesterol (urine bilirubin, urobilinogen) and its application Background technique [0002] Liver disease is a disease with high incidence. The liver has complex physiological functions and is involved in the metabolism of almost all substances in the body. Due to the large volume of the liver (about 3 kg for adults), it has a strong compensatory ability and a very strong ability to regenerate liver cells. If there is no diffuse lesion or only localized damage, the general liver function test will not have obvious changes. [0003] The excretion of "two gallbladders" in urine is closely related to the whole bilirubin metabolism in the body, and it is one of the methods to reflect the liver and gallbladder function. [0004] If the liver parenchyma has lesions, the uptake, combination and excretion of bilirubin can be obstructed. Intra...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/52
Inventor 赵青华
Owner 赵青华
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